Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period

Abstract

A 4-year-old child born to an HIV-1 seronegative mother was diagnosed with HIV-1, the main risk factor being transmission from the child's father who was seroconverting at the time of the child's birth. In the context of a forensic investigation, here we aimed to identify the source of infection of the child and date of the transmission event. Samples were collected from the father and child at two time points about 4 years after the child's birth. Partial segments of three HIV-1 genes (gag, pol, and env) were sequenced and maximum likelihood (ML) and Bayesian methods were used to determine direction and estimate date of transmission. Neutralizing antibodies were determined using a single cycle assay. Bayesian trees displayed a paraphyletic–monophyletic topology in all three genomic regions, with the father's host label at the root, which is consistent with father-to-son transmission. ML trees found similar topologies in gag and pol and a monophyletic–monophyletic topology in env. Analysis of the time of the most recent common ancestor of each HIV-1 gene population indicated that the child was infected shortly after the father. Consistent with the infection history, both father and son developed broad and potent HIV-specific neutralizing antibody responses. In conclusion, themore » direction of transmission implicated the father as the source of transmission. Transmission occurred during the seroconversion period when the father was unaware of the infection and was likely accidental. This case shows how genetic, phylogenetic, and serological data can contribute for the forensic investigation of HIV transmission.« less

Authors:
 [1];  [2];  [2];  [3];  [4]; ORCiD logo [5]; ORCiD logo [5];  [1]
+ Show Author Affiliations
  1. Univ. of Lisbon (Portugal); Inst. Univ. Egas Moniz (IUEM), Monte de Caparica (Portugal)
  2. Univ. of Lisbon (Portugal)
  3. NOVA Univ., Lisbon (Portugal)
  4. Hospital Fernando Fonseca, Amadors (Portugal)
  5. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Laboratory Directed Research and Development (LDRD) Program; National Institutes of Health (NIH); European Funds; Fundacao para a Ciencia e Tecnologia (FCT), Portugal
OSTI Identifier:
1634956
Report Number(s):
LA-UR-19-25850
Journal ID: ISSN 0889-2229
Grant/Contract Number:  
89233218CNA000001; R01AI087520; 249697; PTDC/SAU-EPI/122400/2010; VIH/SAU/0029/2011; PTDC/DTP-EPI/7066/2014; UID/Multi/04413/2013; SFRH/BPD/76225/2011
Resource Type:
Accepted Manuscript
Journal Name:
AIDS Research and Human Retroviruses
Additional Journal Information:
Journal Volume: 34; Journal Issue: 10; Journal ID: ISSN 0889-2229
Publisher:
Mary Ann Liebert, Inc.
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; biological science; HIV father-to-son transmission; forensic investigation of HIV transmission; phylogenetic analysis; neutralizing antibodies

Citation Formats

Ezeonwumelu, Ifeanyi, Bártolo, Inês, Martin, Francisco, Abecasis, Ana, Campos, Teresa, Romero-Severson, Ethan O., Leitner, Thomas, and Taveira, Nuno. Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period. United States: N. p., 2018. Web. doi:10.1089/aid.2018.0060.
Copy to clipboard
Ezeonwumelu, Ifeanyi, Bártolo, Inês, Martin, Francisco, Abecasis, Ana, Campos, Teresa, Romero-Severson, Ethan O., Leitner, Thomas, & Taveira, Nuno. Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period. United States. https://doi.org/10.1089/aid.2018.0060
Copy to clipboard
Ezeonwumelu, Ifeanyi, Bártolo, Inês, Martin, Francisco, Abecasis, Ana, Campos, Teresa, Romero-Severson, Ethan O., Leitner, Thomas, and Taveira, Nuno. Thu . "Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period". United States. https://doi.org/10.1089/aid.2018.0060. https://www.osti.gov/servlets/purl/1634956.
Copy to clipboard
@article{osti_1634956,
title = {Accidental Father-to-Son HIV-1 Transmission During the Seroconversion Period},
author = {Ezeonwumelu, Ifeanyi and Bártolo, Inês and Martin, Francisco and Abecasis, Ana and Campos, Teresa and Romero-Severson, Ethan O. and Leitner, Thomas and Taveira, Nuno},
abstractNote = {A 4-year-old child born to an HIV-1 seronegative mother was diagnosed with HIV-1, the main risk factor being transmission from the child's father who was seroconverting at the time of the child's birth. In the context of a forensic investigation, here we aimed to identify the source of infection of the child and date of the transmission event. Samples were collected from the father and child at two time points about 4 years after the child's birth. Partial segments of three HIV-1 genes (gag, pol, and env) were sequenced and maximum likelihood (ML) and Bayesian methods were used to determine direction and estimate date of transmission. Neutralizing antibodies were determined using a single cycle assay. Bayesian trees displayed a paraphyletic–monophyletic topology in all three genomic regions, with the father's host label at the root, which is consistent with father-to-son transmission. ML trees found similar topologies in gag and pol and a monophyletic–monophyletic topology in env. Analysis of the time of the most recent common ancestor of each HIV-1 gene population indicated that the child was infected shortly after the father. Consistent with the infection history, both father and son developed broad and potent HIV-specific neutralizing antibody responses. In conclusion, the direction of transmission implicated the father as the source of transmission. Transmission occurred during the seroconversion period when the father was unaware of the infection and was likely accidental. This case shows how genetic, phylogenetic, and serological data can contribute for the forensic investigation of HIV transmission.},
doi = {10.1089/aid.2018.0060},
journal = {AIDS Research and Human Retroviruses},
number = 10,
volume = 34,
place = {United States},
year = {2018},
month = {9}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1089/aid.2018.0060
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Citation Metrics:
Cited by: 1 work
Citation information provided by
Web of Science
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

Genetic relatedness of human immunodeficiency virus-1 (HIV-1) strains in a 12-year-old daughter and her father in a household setting
journal, January 2014

  • Shao, Jiasheng; Wang, Jiangrong; Abubakar, Yassir F.
  • Archives of Virology, Vol. 159, Issue 6
  • DOI: 10.1007/s00705-013-1963-5

Father to child transmission of human immunodeficiency virus disease while sero-discordant status of the mother is maintained
journal, January 2013

  • Murugan, Sankaranantham; Anburajan, Rajanayagam
  • Indian Journal of Sexually Transmitted Diseases and AIDS, Vol. 34, Issue 1
  • DOI: 10.4103/0253-7184.112945

HIV-1 and HCV sequences from Libyan outbreak
journal, December 2006

  • de Oliveira, Tulio; Pybus, Oliver G.; Rambaut, Andrew
  • Nature, Vol. 444, Issue 7121
  • DOI: 10.1038/444836a

The tainted milk of human kindness
journal, August 2012

Premastication as a Route of Pediatric HIV Transmission: Case–Control and Cross-Sectional Investigations
journal, January 2012

  • Ivy, Wade; Dominguez, Kenneth L.; Rakhmanina, Natella Y.
  • JAIDS Journal of Acquired Immune Deficiency Syndromes, Vol. 59, Issue 2
  • DOI: 10.1097/QAI.0b013e31823b4554

Human Immunodeficiency Virus Type 1 Infection after Sexual Abuse: Value of Nucleic Acid Sequence Analysis in Identifying the Offender
journal, October 2000

  • Banaschak, Sibylle; Werwein, Marianne; Brinkmann, Bernd
  • Clinical Infectious Diseases, Vol. 31, Issue 4
  • DOI: 10.1086/318152

Donor-Recipient Identification in Para- and Poly-phyletic Trees Under Alternative HIV-1 Transmission Hypotheses Using Approximate Bayesian Computation
journal, September 2017

MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability
journal, January 2013

  • Katoh, K.; Standley, D. M.
  • Molecular Biology and Evolution, Vol. 30, Issue 4
  • DOI: 10.1093/molbev/mst010

MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets
journal, March 2016

  • Kumar, Sudhir; Stecher, Glen; Tamura, Koichiro
  • Molecular Biology and Evolution, Vol. 33, Issue 7
  • DOI: 10.1093/molbev/msw054

Automated subtyping of HIV-1 genetic sequences for clinical and surveillance purposes: Performance evaluation of the new REGA version 3 and seven other tools
journal, October 2013

  • Pineda-Peña, Andrea-Clemencia; Faria, Nuno Rodrigues; Imbrechts, Stijn
  • Infection, Genetics and Evolution, Vol. 19
  • DOI: 10.1016/j.meegid.2013.04.032

A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood
journal, October 2003

SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building
journal, October 2009

  • Gouy, M.; Guindon, S.; Gascuel, O.
  • Molecular Biology and Evolution, Vol. 27, Issue 2
  • DOI: 10.1093/molbev/msp259

Approximate Likelihood-Ratio Test for Branches: A Fast, Accurate, and Powerful Alternative
journal, August 2006

MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space
journal, February 2012

  • Ronquist, Fredrik; Teslenko, Maxim; van der Mark, Paul
  • Systematic Biology, Vol. 61, Issue 3
  • DOI: 10.1093/sysbio/sys029

jModelTest 2: more models, new heuristics and parallel computing
journal, July 2012

  • Darriba, Diego; Taboada, Guillermo L.; Doallo, Ramón
  • Nature Methods, Vol. 9, Issue 8
  • DOI: 10.1038/nmeth.2109

Bayesian Phylogenetics with BEAUti and the BEAST 1.7
journal, February 2012

  • Drummond, Alexei J.; Suchard, Marc A.; Xie, Dong
  • Molecular Biology and Evolution, Vol. 29, Issue 8
  • DOI: 10.1093/molbev/mss075

Improving Marginal Likelihood Estimation for Bayesian Phylogenetic Model Selection
journal, December 2010

Phylogenetically resolving epidemiologic linkage
journal, February 2016

  • Romero-Severson, Ethan O.; Bulla, Ingo; Leitner, Thomas
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 10
  • DOI: 10.1073/pnas.1522930113

Datamonkey 2010: a suite of phylogenetic analysis tools for evolutionary biology
journal, July 2010

Global Panel of HIV-1 Env Reference Strains for Standardized Assessments of Vaccine-Elicited Neutralizing Antibodies
journal, December 2013

  • deCamp, A.; Hraber, P.; Bailer, R. T.
  • Journal of Virology, Vol. 88, Issue 5
  • DOI: 10.1128/JVI.02853-13

HIV-1 in blister fluid of a patient with toxic epidermal necrolysis and AIDS
journal, November 1994

Horizontal Transmission of Human Immunodeficiency Virus Type 1 from Father to Child
journal, December 1998

  • Salvatori, Francesca; De Martino, Maurizio; Galli, Luisa
  • AIDS Research and Human Retroviruses, Vol. 14, Issue 18
  • DOI: 10.1089/aid.1998.14.1679

Review: influence of ART on HIV genetics
journal, January 2015

Immunologic characteristics of HIV-infected individuals who make broadly neutralizing antibodies
journal, January 2017

  • Borrow, Persephone; Moody, M. Anthony
  • Immunological Reviews, Vol. 275, Issue 1
  • DOI: 10.1111/imr.12504
    Sort by:
    [ × clear filter / sort ]

    Similar Records in DOE PAGES and OSTI.GOV collections: