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Title: Effects of Arsenic on Trichloroethene–Dechlorination Activities of Dehalococcoides mccartyi 195

Abstract

Arsenic and trichloroethene (TCE) are among the most prevalent groundwater contaminants in the United States. Co-contamination of these two compounds has been detected at 63% of current TCE-contaminated National Priorities List sites. When in situ TCE reductive dechlorination is stimulated by the addition of fermentable substrates to generate a reducing environment, the presence of arsenic can be problematic because of the potential for increased mobilization and toxicity caused by the reduction of arsenate [As(V)] to arsenite [As(III)]. This study assesses the effects of arsenic exposure on the TCE-dechlorinating activities of Dehalococcoides mccartyi strain 195. Here, our results indicate that 9.1 μM As(III) caused a 50% decrease in D. mccartyi cell growth. While As(V) concentrations up to 200 μM did not initially impact TCE dechlorination, inhibition was observed in cultures amended with 200 μM As(V) and 100 μM As(V) in 12 and 17 days, respectively, corresponding with the accumulation of As(III). Transcriptomic and metabolomic analyses were performed to evaluate cellular responses to both As(V) and As(III) stress. Amendment of amino acids enhanced arsenic tolerance of D. mccartyi. Results from this study improve our understanding of potential inhibitions of D. mccartyi metabolism caused by arsenic and can inform the design of bioremediationmore » strategies at co-contaminated sites.« less

Authors:
ORCiD logo [1];  [2]
  1. Univ. of California, Berkeley, CA (United States)
  2. Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
National Institute of Environmental Health Sciences (NIEHS)
OSTI Identifier:
1633258
Grant/Contract Number:  
AC02-05CH11231; P42ES004705
Resource Type:
Accepted Manuscript
Journal Name:
Environmental Science and Technology
Additional Journal Information:
Journal Volume: 54; Journal Issue: 2; Journal ID: ISSN 0013-936X
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Gushgari-Doyle, Sara, and Alvarez-Cohen, Lisa. Effects of Arsenic on Trichloroethene–Dechlorination Activities of Dehalococcoides mccartyi 195. United States: N. p., 2020. Web. https://doi.org/10.1021/acs.est.9b06527.
Gushgari-Doyle, Sara, & Alvarez-Cohen, Lisa. Effects of Arsenic on Trichloroethene–Dechlorination Activities of Dehalococcoides mccartyi 195. United States. https://doi.org/10.1021/acs.est.9b06527
Gushgari-Doyle, Sara, and Alvarez-Cohen, Lisa. Wed . "Effects of Arsenic on Trichloroethene–Dechlorination Activities of Dehalococcoides mccartyi 195". United States. https://doi.org/10.1021/acs.est.9b06527. https://www.osti.gov/servlets/purl/1633258.
@article{osti_1633258,
title = {Effects of Arsenic on Trichloroethene–Dechlorination Activities of Dehalococcoides mccartyi 195},
author = {Gushgari-Doyle, Sara and Alvarez-Cohen, Lisa},
abstractNote = {Arsenic and trichloroethene (TCE) are among the most prevalent groundwater contaminants in the United States. Co-contamination of these two compounds has been detected at 63% of current TCE-contaminated National Priorities List sites. When in situ TCE reductive dechlorination is stimulated by the addition of fermentable substrates to generate a reducing environment, the presence of arsenic can be problematic because of the potential for increased mobilization and toxicity caused by the reduction of arsenate [As(V)] to arsenite [As(III)]. This study assesses the effects of arsenic exposure on the TCE-dechlorinating activities of Dehalococcoides mccartyi strain 195. Here, our results indicate that 9.1 μM As(III) caused a 50% decrease in D. mccartyi cell growth. While As(V) concentrations up to 200 μM did not initially impact TCE dechlorination, inhibition was observed in cultures amended with 200 μM As(V) and 100 μM As(V) in 12 and 17 days, respectively, corresponding with the accumulation of As(III). Transcriptomic and metabolomic analyses were performed to evaluate cellular responses to both As(V) and As(III) stress. Amendment of amino acids enhanced arsenic tolerance of D. mccartyi. Results from this study improve our understanding of potential inhibitions of D. mccartyi metabolism caused by arsenic and can inform the design of bioremediation strategies at co-contaminated sites.},
doi = {10.1021/acs.est.9b06527},
journal = {Environmental Science and Technology},
number = 2,
volume = 54,
place = {United States},
year = {2020},
month = {1}
}

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