Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla
Abstract
Plant-associated microbial communities have diverse phenotypic effects on their hosts that are only beginning to be revealed. We hypothesized that morpho-physiological variations in the tropical tree Tabebuia heterophylla, observed on different geological substrates, arise in part due to microbial processes in the rhizosphere. We characterized the microbiota of the rhizosphere and soil communities associated with T. heterophylla trees in high and low altitude sites (with varying temperature and precipitation) of volcanic, karst and serpentine geologies across Puerto Rico. We sampled 6 areas across the island in three geological materials including volcanic, serpentine and karst soils. Collection was done in 2 elevations (>450m and 0-300m high), that included 3 trees for each site and 4 replicate soil samples per tree of both bulk and rhizosphere. Genomic DNA was extracted from 144 samples, and 16S rRNA V4 sequencing was performed on the Illumina MiSeq platform. Proteobacteria, Actinobacteria, and Verrucomicrobia were the most dominant phyla, and microbiomes clustered by geological substrate and elevation. Volcanic samples were enriched in Verrucomicrobia; karst was dominated by nitrogen-fixing Proteobacteria, and serpentine sites harbored the most diverse communities, with dominant Cyanobacteria. Sites with similar climates but differing geologies showed significant differences on rhizobiota diversity and composition demonstrating themore »
- Authors:
-
[1];
[1]
- Univ. of Puerto Rico, San Juan, PR (United States)
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Science Foundation (NSF); National Institutes of Health (NIH)
- OSTI Identifier:
- 1633254
- Grant/Contract Number:
- AC02-05CH11231; NSF-HRD 0734826; U54 MD007600
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 4; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Ortiz, Yakshi, Restrepo, Carla, Vilanova-Cuevas, Brayan, Santiago-Valentin, Eugenio, Tringe, Susannah G., and Godoy-Vitorino, Filipa. Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla. United States: N. p., 2020.
Web. doi:10.1371/journal.pone.0231083.
Ortiz, Yakshi, Restrepo, Carla, Vilanova-Cuevas, Brayan, Santiago-Valentin, Eugenio, Tringe, Susannah G., & Godoy-Vitorino, Filipa. Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla. United States. https://doi.org/10.1371/journal.pone.0231083
Ortiz, Yakshi, Restrepo, Carla, Vilanova-Cuevas, Brayan, Santiago-Valentin, Eugenio, Tringe, Susannah G., and Godoy-Vitorino, Filipa. Tue .
"Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla". United States. https://doi.org/10.1371/journal.pone.0231083. https://www.osti.gov/servlets/purl/1633254.
@article{osti_1633254,
title = {Geology and climate influence rhizobiome composition of the phenotypically diverse tropical tree Tabebuia heterophylla},
author = {Ortiz, Yakshi and Restrepo, Carla and Vilanova-Cuevas, Brayan and Santiago-Valentin, Eugenio and Tringe, Susannah G. and Godoy-Vitorino, Filipa},
abstractNote = {Plant-associated microbial communities have diverse phenotypic effects on their hosts that are only beginning to be revealed. We hypothesized that morpho-physiological variations in the tropical tree Tabebuia heterophylla, observed on different geological substrates, arise in part due to microbial processes in the rhizosphere. We characterized the microbiota of the rhizosphere and soil communities associated with T. heterophylla trees in high and low altitude sites (with varying temperature and precipitation) of volcanic, karst and serpentine geologies across Puerto Rico. We sampled 6 areas across the island in three geological materials including volcanic, serpentine and karst soils. Collection was done in 2 elevations (>450m and 0-300m high), that included 3 trees for each site and 4 replicate soil samples per tree of both bulk and rhizosphere. Genomic DNA was extracted from 144 samples, and 16S rRNA V4 sequencing was performed on the Illumina MiSeq platform. Proteobacteria, Actinobacteria, and Verrucomicrobia were the most dominant phyla, and microbiomes clustered by geological substrate and elevation. Volcanic samples were enriched in Verrucomicrobia; karst was dominated by nitrogen-fixing Proteobacteria, and serpentine sites harbored the most diverse communities, with dominant Cyanobacteria. Sites with similar climates but differing geologies showed significant differences on rhizobiota diversity and composition demonstrating the importance of geology in shaping the rhizosphere microbiota, with implications for the plant's phenotype. Our study sheds light on the combined role of geology and climate in the rhizosphere microbial consortia, likely contributing to the phenotypic plasticity of the trees.},
doi = {10.1371/journal.pone.0231083},
journal = {PLoS ONE},
number = 4,
volume = 15,
place = {United States},
year = {Tue Apr 07 00:00:00 EDT 2020},
month = {Tue Apr 07 00:00:00 EDT 2020}
}
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