Nitrogen Source Governs Community Carbon Metabolism in a Model Hypersaline Benthic Phototrophic Biofilm
Abstract
Increasing anthropogenic inputs of fixed nitrogen are leading to greater eutrophication of aquatic environments, but it is unclear how this impacts the flux and fate of carbon in lacustrine and riverine systems. Here, we present evidence that the form of nitrogen governs the partitioning of carbon among members in a genome-sequenced, model phototrophic biofilm of 20 members. Consumption of NO3– as the sole nitrogen source unexpectedly resulted in more rapid transfer of carbon to heterotrophs than when NH4+ was also provided, suggesting alterations in the form of carbon exchanged. The form of nitrogen dramatically impacted net community nitrogen, but not carbon, uptake rates. Furthermore, this alteration in nitrogen form caused very large but focused alterations to community structure, strongly impacting the abundance of only two species within the biofilm and modestly impacting a third member species. Our data suggest that nitrogen metabolism may coordinate coupled carbon-nitrogen biogeochemical cycling in benthic biofilms and, potentially, in phototroph-heterotroph consortia more broadly. It further indicates that the form of nitrogen inputs may significantly impact the contribution of these communities to carbon partitioning across the terrestrial-aquatic interface.
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1632235
- Alternate Identifier(s):
- OSTI ID: 1647036
- Report Number(s):
- PNNL-SA-145330
Journal ID: ISSN 2379-5077; e00260-20
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- mSystems
- Additional Journal Information:
- Journal Name: mSystems Journal Volume: 5 Journal Issue: 3; Journal ID: ISSN 2379-5077
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- carbon cycling; cyanobacteria; mass spectrometry; nitrogen cycling; stable isotopes
Citation Formats
Anderton, Christopher R., Mobberley, Jennifer M., Cole, Jessica K., Nunez, Jamie R., Starke, Robert, Boaro, Amy A., Yesiltepe, Yasemin, Morton, Beau R., Cory, Alexandra B., Cardamone, Hayley C., Hofmockel, Kirsten S., Lipton, Mary S., Moran, James J., Renslow, Ryan S., Fredrickson, James K., Lindemann, Stephen R., and Hallam, ed., Steven J. Nitrogen Source Governs Community Carbon Metabolism in a Model Hypersaline Benthic Phototrophic Biofilm. United States: N. p., 2020.
Web. doi:10.1128/mSystems.00260-20.
Anderton, Christopher R., Mobberley, Jennifer M., Cole, Jessica K., Nunez, Jamie R., Starke, Robert, Boaro, Amy A., Yesiltepe, Yasemin, Morton, Beau R., Cory, Alexandra B., Cardamone, Hayley C., Hofmockel, Kirsten S., Lipton, Mary S., Moran, James J., Renslow, Ryan S., Fredrickson, James K., Lindemann, Stephen R., & Hallam, ed., Steven J. Nitrogen Source Governs Community Carbon Metabolism in a Model Hypersaline Benthic Phototrophic Biofilm. United States. https://doi.org/10.1128/mSystems.00260-20
Anderton, Christopher R., Mobberley, Jennifer M., Cole, Jessica K., Nunez, Jamie R., Starke, Robert, Boaro, Amy A., Yesiltepe, Yasemin, Morton, Beau R., Cory, Alexandra B., Cardamone, Hayley C., Hofmockel, Kirsten S., Lipton, Mary S., Moran, James J., Renslow, Ryan S., Fredrickson, James K., Lindemann, Stephen R., and Hallam, ed., Steven J. Tue .
"Nitrogen Source Governs Community Carbon Metabolism in a Model Hypersaline Benthic Phototrophic Biofilm". United States. https://doi.org/10.1128/mSystems.00260-20.
@article{osti_1632235,
title = {Nitrogen Source Governs Community Carbon Metabolism in a Model Hypersaline Benthic Phototrophic Biofilm},
author = {Anderton, Christopher R. and Mobberley, Jennifer M. and Cole, Jessica K. and Nunez, Jamie R. and Starke, Robert and Boaro, Amy A. and Yesiltepe, Yasemin and Morton, Beau R. and Cory, Alexandra B. and Cardamone, Hayley C. and Hofmockel, Kirsten S. and Lipton, Mary S. and Moran, James J. and Renslow, Ryan S. and Fredrickson, James K. and Lindemann, Stephen R. and Hallam, ed., Steven J.},
abstractNote = {Increasing anthropogenic inputs of fixed nitrogen are leading to greater eutrophication of aquatic environments, but it is unclear how this impacts the flux and fate of carbon in lacustrine and riverine systems. Here, we present evidence that the form of nitrogen governs the partitioning of carbon among members in a genome-sequenced, model phototrophic biofilm of 20 members. Consumption of NO3– as the sole nitrogen source unexpectedly resulted in more rapid transfer of carbon to heterotrophs than when NH4+ was also provided, suggesting alterations in the form of carbon exchanged. The form of nitrogen dramatically impacted net community nitrogen, but not carbon, uptake rates. Furthermore, this alteration in nitrogen form caused very large but focused alterations to community structure, strongly impacting the abundance of only two species within the biofilm and modestly impacting a third member species. Our data suggest that nitrogen metabolism may coordinate coupled carbon-nitrogen biogeochemical cycling in benthic biofilms and, potentially, in phototroph-heterotroph consortia more broadly. It further indicates that the form of nitrogen inputs may significantly impact the contribution of these communities to carbon partitioning across the terrestrial-aquatic interface.},
doi = {10.1128/mSystems.00260-20},
journal = {mSystems},
number = 3,
volume = 5,
place = {United States},
year = {2020},
month = {6}
}
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