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Title: In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging

Abstract

Imaging biological systems with simultaneous intrinsic chemical specificity and nanometer spatial resolution in their typical native liquid environment has remained a long-standing challenge. In this paper we demonstrate a general approach of chemical nanoimaging in liquid based on infrared scattering scanning near-field optical microscopy (IR $s$-SNOM). It is enabled by combining AFM operation in a fluid cell with evanescent IR illumination via total internal reflection, which provides spatially confined excitation for minimized IR water absorption, reduced far-field background, and enhanced directional signal emission and sensitivity. We demonstrate in-liquid IR $s$-SNOM vibrational nanoimaging and conformational identification of catalase nano-crystals and spatio-spectral analysis of biomimetic peptoid sheets with monolayer sensitivity and chemical specificity at the few zeptomole level. This work establishes the principles of in-liquid and in-situ IR $s$-SNOM spectroscopic chemical nano-imaging and its general applicability to biomolecular, cellular, catalytic, electrochemical, or other interfaces and nano-systems in liquids or solutions.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [1]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [3]; ORCiD logo [4]; ORCiD logo [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
  2. Ulsan National Inst. of Science and Technology (UNIST) (Republic of Korea)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Univ. of Colorado, Boulder, CO (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES); National Research Foundation of Korea (NRF)
OSTI Identifier:
1631996
Report Number(s):
PNNL-SA-152805
Journal ID: ISSN 1530-6984
Grant/Contract Number:  
AC05-76RL01830; DMR 1548924; NRF-2019R1F1A1059892
Resource Type:
Accepted Manuscript
Journal Name:
Nano Letters
Additional Journal Information:
Journal Volume: 20; Journal Issue: 6; Journal ID: ISSN 1530-6984
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
English
Subject:
nano-spectroscopy; bio-nanoimaging; in-liquid; in-situ

Citation Formats

O’Callahan, Brian T., Park, Kyoung-Duck, Novikova, Irina V., Jian, Tengyue, Chen, Chun-Long, Muller, Eric A., El-Khoury, Patrick Z., Raschke, Markus B., and Lea, A. Scott. In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging. United States: N. p., 2020. Web. doi:10.1021/acs.nanolett.0c01291.
O’Callahan, Brian T., Park, Kyoung-Duck, Novikova, Irina V., Jian, Tengyue, Chen, Chun-Long, Muller, Eric A., El-Khoury, Patrick Z., Raschke, Markus B., & Lea, A. Scott. In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging. United States. doi:10.1021/acs.nanolett.0c01291.
O’Callahan, Brian T., Park, Kyoung-Duck, Novikova, Irina V., Jian, Tengyue, Chen, Chun-Long, Muller, Eric A., El-Khoury, Patrick Z., Raschke, Markus B., and Lea, A. Scott. Fri . "In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging". United States. doi:10.1021/acs.nanolett.0c01291.
@article{osti_1631996,
title = {In Liquid Infrared Scattering Scanning Near-Field Optical Microscopy for Chemical and Biological Nanoimaging},
author = {O’Callahan, Brian T. and Park, Kyoung-Duck and Novikova, Irina V. and Jian, Tengyue and Chen, Chun-Long and Muller, Eric A. and El-Khoury, Patrick Z. and Raschke, Markus B. and Lea, A. Scott},
abstractNote = {Imaging biological systems with simultaneous intrinsic chemical specificity and nanometer spatial resolution in their typical native liquid environment has remained a long-standing challenge. In this paper we demonstrate a general approach of chemical nanoimaging in liquid based on infrared scattering scanning near-field optical microscopy (IR $s$-SNOM). It is enabled by combining AFM operation in a fluid cell with evanescent IR illumination via total internal reflection, which provides spatially confined excitation for minimized IR water absorption, reduced far-field background, and enhanced directional signal emission and sensitivity. We demonstrate in-liquid IR $s$-SNOM vibrational nanoimaging and conformational identification of catalase nano-crystals and spatio-spectral analysis of biomimetic peptoid sheets with monolayer sensitivity and chemical specificity at the few zeptomole level. This work establishes the principles of in-liquid and in-situ IR $s$-SNOM spectroscopic chemical nano-imaging and its general applicability to biomolecular, cellular, catalytic, electrochemical, or other interfaces and nano-systems in liquids or solutions.},
doi = {10.1021/acs.nanolett.0c01291},
journal = {Nano Letters},
number = 6,
volume = 20,
place = {United States},
year = {2020},
month = {5}
}

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