DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production

; ; ; ; ;
Publication Date:
Sponsoring Org.:
OSTI Identifier:
Resource Type:
Published Article
Journal Name:
Frontiers in Energy Research
Additional Journal Information:
Journal Name: Frontiers in Energy Research Journal Volume: 8; Journal ID: ISSN 2296-598X
Frontiers Media SA
Country of Publication:

Citation Formats

Cui, Jingxuan, Stevenson, David, Korosh, Travis, Amador-Noguez, Daniel, Olson, Daniel G., and Lynd, Lee R. Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production. Switzerland: N. p., 2020. Web. doi:10.3389/fenrg.2020.00072.
Cui, Jingxuan, Stevenson, David, Korosh, Travis, Amador-Noguez, Daniel, Olson, Daniel G., & Lynd, Lee R. Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production. Switzerland.
Cui, Jingxuan, Stevenson, David, Korosh, Travis, Amador-Noguez, Daniel, Olson, Daniel G., and Lynd, Lee R. Thu . "Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production". Switzerland.
title = {Developing a Cell-Free Extract Reaction (CFER) System in Clostridium thermocellum to Identify Metabolic Limitations to Ethanol Production},
author = {Cui, Jingxuan and Stevenson, David and Korosh, Travis and Amador-Noguez, Daniel and Olson, Daniel G. and Lynd, Lee R.},
abstractNote = {},
doi = {10.3389/fenrg.2020.00072},
journal = {Frontiers in Energy Research},
number = ,
volume = 8,
place = {Switzerland},
year = {2020},
month = {6}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Biosynthesis of ε-poly-l-lysine in a cell-free system of Streptomyces albulus
journal, November 2003

  • Kawai, Takahiro; Kubota, Takaaki; Hiraki, Jun
  • Biochemical and Biophysical Research Communications, Vol. 311, Issue 3
  • DOI: 10.1016/j.bbrc.2003.10.033

Glycolysis without pyruvate kinase in Clostridium thermocellum
journal, January 2017

Optimization of a blueprint for in vitro glycolysis by metabolic real-time analysis
journal, March 2011

  • Bujara, Matthias; Schümperli, Michael; Pellaux, René
  • Nature Chemical Biology, Vol. 7, Issue 5
  • DOI: 10.1038/nchembio.541

Metabolite Measurement: Pitfalls to Avoid and Practices to Follow
journal, June 2017

Analysis of composition and structure of Clostridium thermocellum membranes from wild-type and ethanol-adapted strains
journal, February 2009

  • Timmons, Michael D.; Knutson, Barbara L.; Nokes, Sue E.
  • Applied Microbiology and Biotechnology, Vol. 82, Issue 5
  • DOI: 10.1007/s00253-009-1891-1

Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum.
journal, January 1982

Fermentation capabilities ofZymomonas mobilis glycolytic enzymes
journal, September 1986

  • Scopes, R. K.; Griffiths-Smith, K.
  • Biotechnology Letters, Vol. 8, Issue 9
  • DOI: 10.1007/BF01025976

Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway
journal, January 2012

High-throughput preparation methods of crude extract for robust cell-free protein synthesis
journal, March 2015

  • Kwon, Yong-Chan; Jewett, Michael C.
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep08663

Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways
journal, May 2013

  • van der Veen, Douwe; Lo, Jonathan; Brown, Steven D.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 7
  • DOI: 10.1007/s10295-013-1275-5

In vitro production of n-butanol from glucose
journal, November 2013

Preparative, in Vitro Biocatalysis of Triketide Lactone Chiral Building Blocks
journal, September 2012

  • Harper, Andrew D.; Bailey, Constance B.; Edwards, Angela D.
  • ChemBioChem, Vol. 13, Issue 15
  • DOI: 10.1002/cbic.201200378

Cell-free production of isobutanol: A completely immobilized system
journal, December 2019

Proteomic profile changes in membranes of ethanol-tolerant Clostridium thermocellum
journal, November 2006

  • Williams, Taufika Islam; Combs, Jennifer C.; Lynn, Bert C.
  • Applied Microbiology and Biotechnology, Vol. 74, Issue 2
  • DOI: 10.1007/s00253-006-0689-7

Functional Genomics: Expression Analysis ofEscherichia coli Growing on Minimal and Rich Media
journal, October 1999

Studies on cell-free metabolism: Ethanol production by extracts of Zymomonas mobilis
journal, July 1985

Cell-Free Mixing of Escherichia coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis
journal, July 2016

  • Dudley, Quentin M.; Anderson, Kim C.; Jewett, Michael C.
  • ACS Synthetic Biology, Vol. 5, Issue 12
  • DOI: 10.1021/acssynbio.6b00154

Recent progress in consolidated bioprocessing
journal, June 2012

  • Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
  • Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
  • DOI: 10.1016/j.copbio.2011.11.026

Lysate of engineered Escherichia coli supports high-level conversion of glucose to 2,3-butanediol
journal, November 2015

Development of a core Clostridium thermocellum kinetic metabolic model consistent with multiple genetic perturbations
journal, May 2017

Bacteria engineered for fuel ethanol production: current status
journal, December 2003

  • Dien, B. S.; Cotta, M. A.; Jeffries, T. W.
  • Applied Microbiology and Biotechnology, Vol. 63, Issue 3, p. 258-266
  • DOI: 10.1007/s00253-003-1444-y

Atypical Glycolysis in Clostridium thermocellum
journal, February 2013

  • Zhou, Jilai; Olson, Daniel G.; Argyros, D. Aaron
  • Applied and Environmental Microbiology, Vol. 79, Issue 9, p. 3000-3008
  • DOI: 10.1128/AEM.04037-12

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli
journal, June 2009

  • Bennett, Bryson D.; Kimball, Elizabeth H.; Gao, Melissa
  • Nature Chemical Biology, Vol. 5, Issue 8
  • DOI: 10.1038/nchembio.186

Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
journal, June 2016

Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol
journal, November 2017

  • Tian, Liang; Perot, Skyler J.; Stevenson, David
  • Biotechnology for Biofuels, Vol. 10, Issue 1
  • DOI: 10.1186/s13068-017-0961-3

Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production
journal, September 2018

  • Hon, Shuen; Holwerda, Evert K.; Worthen, Robert S.
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1245-2

Global Metabolic Profiling of Escherichia coli Cultures:  an Evaluation of Methods for Quenching and Extraction of Intracellular Metabolites
journal, April 2008

  • Winder, Catherine L.; Dunn, Warwick B.; Schuler, Stephanie
  • Analytical Chemistry, Vol. 80, Issue 8
  • DOI: 10.1021/ac7023409

Synthetic non-oxidative glycolysis enables complete carbon conservation
journal, September 2013

  • Bogorad, Igor W.; Lin, Tzu-Shyang; Liao, James C.
  • Nature, Vol. 502, Issue 7473
  • DOI: 10.1038/nature12575

Studies on cell-free metabolism: Ethanol production by a yeast glycolytic system reconstituted from purified enzymes
journal, July 1985

Thermodynamic analysis of the pathway for ethanol production from cellobiose in Clostridium thermocellum
journal, September 2019

Reassessment of the Transhydrogenase/Malate Shunt Pathway in Clostridium thermocellum ATCC 27405 through Kinetic Characterization of Malic Enzyme and Malate Dehydrogenase
journal, January 2015

  • Taillefer, M.; Rydzak, T.; Levin, D. B.
  • Applied and Environmental Microbiology, Vol. 81, Issue 7
  • DOI: 10.1128/AEM.03360-14

A synthetic biochemistry platform for cell free production of monoterpenes from glucose
journal, May 2017

  • Korman, Tyler P.; Opgenorth, Paul H.; Bowie, James U.
  • Nature Communications, Vol. 8, Issue 1
  • DOI: 10.1038/ncomms15526

In Vitro Biosynthesis of the Antitumor Agent Azinomycin B
journal, March 2006

  • Liu, Chaomin; Kelly, Gilbert T.; Watanabe, Coran M. H.
  • Organic Letters, Vol. 8, Issue 6
  • DOI: 10.1021/ol052987l

Yeast cell-free enzyme system for bio-ethanol production at elevated temperatures
journal, March 2014

Synthesis of non-natural carbohydrates from glycerol and aldehydes in a one-pot four-enzyme cascade reaction
journal, January 2011

  • Babich, Lara; van Hemert, Lieke J. C.; Bury, Aleksandra
  • Green Chemistry, Vol. 13, Issue 10
  • DOI: 10.1039/c1gc15429j

2H and 13C metabolic flux analysis elucidates in vivo thermodynamics of the ED pathway in Zymomonas mobilis
journal, July 2019

A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
journal, July 2016

Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
journal, October 2012

Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide
journal, March 2013

  • Keller, M. W.; Schut, G. J.; Lipscomb, G. L.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 15, p. 5840-5845
  • DOI: 10.1073/pnas.1222607110

Cell-free synthetic biology: Thinking outside the cell
journal, May 2012

A cell-free platform for the prenylation of natural products and application to cannabinoid production
journal, February 2019

  • Valliere, Meaghan A.; Korman, Tyler P.; Woodall, Nicholas B.
  • Nature Communications, Vol. 10, Issue 1
  • DOI: 10.1038/s41467-019-08448-y

Cloning and expression of the Clostridium thermocellum L-lactate dehydrogenase gene in Escherichia coli and enzyme characterization
journal, October 2004

  • Özkan, Melek; Yllmaz, Ebru I.; Lynd, Lee R.
  • Canadian Journal of Microbiology, Vol. 50, Issue 10
  • DOI: 10.1139/w04-071

The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
journal, October 2014

  • Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G.
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/s13068-014-0155-1

Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum
journal, August 2011

  • Shao, Xiongjun; Raman, Babu; Zhu, Mingjun
  • Applied Microbiology and Biotechnology, Vol. 92, Issue 3
  • DOI: 10.1007/s00253-011-3492-z

In vitro reconstitution of mevalonate pathway and targeted engineering of farnesene overproduction in Escherichia coli : In Vitro Reconstitution of Mevalonate Pathway
journal, February 2014

  • Zhu, Fayin; Zhong, Xiaofang; Hu, Mengzhu
  • Biotechnology and Bioengineering, Vol. 111, Issue 7
  • DOI: 10.1002/bit.25198

Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum
journal, August 2011

  • Brown, S. D.; Guss, A. M.; Karpinets, T. V.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 33
  • DOI: 10.1073/pnas.1102444108

Fermentative glycolysis with purified Escherichia coli enzymes for in vitro ATP production and evaluating an engineered enzyme
journal, January 2012