Title: Total internal reflection enabled wide-field coherent anti-Stokes Raman scattering microscopy

Abstract

Wide-field coherent anti-Stokes Raman scattering (CARS) microscopy offers an attractive means for the rapid and simultaneous acquisition of vibrationally resolved images across a large field of view. A major challenge in the implementation lies in how to achieve sufficiently strong excitation fields necessary to drive the third-order optical responses over the large focal region. Here, we report a new wide-field CARS microscope enabled by a total internal reflection excitation scheme using a femtosecond Ti:Sapphire oscillator to generate pump and broadband near-infrared Stokes pulses. The spectrally broad Stokes pulse, in combination with its inherent chirp, offers not only access to a wide range of Raman modes spanning $\sim \; <\#comment/>1000$ to $\sim \; <\#comment/>3500{\mathrm{c}\mathrm{m}}^{-\; <\#comment/>1}$ but also a straightforward means to select vibrational transitions within this range by simply varying the time delay between the pulses. The unique capabilities of this wide-field CARS microscope were validated by acquiring high-quality CARS images from the model and complex biological samples on conventional microscope coverslips.

Authors:

Doughty, Benjamin ;

• Search DOE PAGES for author "Doughty, Benjamin"
• Search DOE PAGES for ORCID "0000-0001-6429-9329"

Premadasa, Uvinduni I.; Cahill, John F.; Webb, Amber B.; Morrell-Falvey, Jennifer L.; Khalid, Muneeba; Retterer, Scott T.; Ma, Ying-Zhong

• Search DOE PAGES for author "Ma, Ying-Zhong"
• Search DOE PAGES for ORCID "0000-0002-8154-1006"

Publication Date:

Thu May 28 00:00:00 EDT 2020

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:

1631503

Alternate Identifier(s):

OSTI ID: 1631504

Resource Type:

Publisher's Accepted Manuscript

Journal Name:

Optics Letters

Additional Journal Information:

Journal Name: Optics Letters Journal Volume: 45 Journal Issue: 11; Journal ID: ISSN 0146-9592

Publisher:

Optical Society of America

Country of Publication:

United States

Language:

English