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Title: Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis hypocotyls

Abstract

Auxin-induced cell elongation relies in part on the acidification of the cell wall, a process known as acid growth that presumably triggers expansin-mediated wall loosening via altered interactions between cellulose microfibrils. Cellulose microfibrils are a major determinant for anisotropic growth and they provide the scaffold for cell wall assembly. Little is known about how acid growth depends on cell wall architecture. To explore the relationship between acid growth-mediated cell elongation and plant cell wall architecture, two mutants (jia1-1 and csi1-3) that are defective in cellulose biosynthesis and cellulose microfibril organization were analyzed. The study revealed that cell elongation is dependent on CSI1-mediated cell wall architecture but not on the overall crystalline cellulose content. We observed a correlation between loss of crossed-polylamellate walls and loss of auxin- and fusicoccin-induced cell growth in csi1-3. Furthermore, induced loss of crossed-polylamellate walls via disruption of cortical microtubules mimics the effect of csi1 in acid growth. We hypothesize that CSI1- and microtubule-dependent crossed-polylamellate walls are required for acid growth in Arabidopsis hypocotyls.

Authors:
 [1];  [1];  [2];  [2];  [3];  [3];  [2];  [1]; ORCiD logo [1];  [4]
  1. Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, USA
  2. Department of Biology, Pennsylvania State University, University Park, PA, USA
  3. Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
  4. INRAE-University Clermont Auvergne, France
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1631346
Alternate Identifier(s):
OSTI ID: 1767735
Grant/Contract Number:  
SC0001090
Resource Type:
Published Article
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Name: Journal of Experimental Botany Journal Volume: 71 Journal Issue: 10; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biofuels (including algae and biomass); bio-inspired; membrane; carbon sequestration; materials and chemistry by design; synthesis (self-assembly); acid growth; atomic force microscopy; axial elongation; cell wall; crossed-polylamellate walls; electron microscopy; microtubules

Citation Formats

Xin, Xiaoran, Lei, Lei, Zheng, Yunzhen, Zhang, Tian, Pingali, Sai Venkatesh, O’Neill, Hugh, Cosgrove, Daniel J., Li, Shundai, Gu, Ying, and Moulia, ed., Bruno. Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis hypocotyls. United Kingdom: N. p., 2020. Web. doi:10.1093/jxb/eraa063.
Xin, Xiaoran, Lei, Lei, Zheng, Yunzhen, Zhang, Tian, Pingali, Sai Venkatesh, O’Neill, Hugh, Cosgrove, Daniel J., Li, Shundai, Gu, Ying, & Moulia, ed., Bruno. Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis hypocotyls. United Kingdom. doi:https://doi.org/10.1093/jxb/eraa063
Xin, Xiaoran, Lei, Lei, Zheng, Yunzhen, Zhang, Tian, Pingali, Sai Venkatesh, O’Neill, Hugh, Cosgrove, Daniel J., Li, Shundai, Gu, Ying, and Moulia, ed., Bruno. Tue . "Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis hypocotyls". United Kingdom. doi:https://doi.org/10.1093/jxb/eraa063.
@article{osti_1631346,
title = {Cellulose synthase interactive1- and microtubule-dependent cell wall architecture is required for acid growth in Arabidopsis hypocotyls},
author = {Xin, Xiaoran and Lei, Lei and Zheng, Yunzhen and Zhang, Tian and Pingali, Sai Venkatesh and O’Neill, Hugh and Cosgrove, Daniel J. and Li, Shundai and Gu, Ying and Moulia, ed., Bruno},
abstractNote = {Auxin-induced cell elongation relies in part on the acidification of the cell wall, a process known as acid growth that presumably triggers expansin-mediated wall loosening via altered interactions between cellulose microfibrils. Cellulose microfibrils are a major determinant for anisotropic growth and they provide the scaffold for cell wall assembly. Little is known about how acid growth depends on cell wall architecture. To explore the relationship between acid growth-mediated cell elongation and plant cell wall architecture, two mutants (jia1-1 and csi1-3) that are defective in cellulose biosynthesis and cellulose microfibril organization were analyzed. The study revealed that cell elongation is dependent on CSI1-mediated cell wall architecture but not on the overall crystalline cellulose content. We observed a correlation between loss of crossed-polylamellate walls and loss of auxin- and fusicoccin-induced cell growth in csi1-3. Furthermore, induced loss of crossed-polylamellate walls via disruption of cortical microtubules mimics the effect of csi1 in acid growth. We hypothesize that CSI1- and microtubule-dependent crossed-polylamellate walls are required for acid growth in Arabidopsis hypocotyls.},
doi = {10.1093/jxb/eraa063},
journal = {Journal of Experimental Botany},
number = 10,
volume = 71,
place = {United Kingdom},
year = {2020},
month = {2}
}

Journal Article:
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DOI: https://doi.org/10.1093/jxb/eraa063

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