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Title: Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock

Abstract

Current sources of fermentation feedstocks, i.e., corn, sugar cane, or plant biomass, fall short of demand for liquid transportation fuels and commodity chemicals in the United States. Aquatic phototrophs including cyanobacteria have the potential to supplement the supply of current fermentable feedstocks. In our strategy, cells are engineered to accumulate storage molecules including glycogen, cellulose, and/or lipid oils that can be extracted from harvested biomass and fed to heterotrophic organisms engineered to produce desired chemical products. Here in this manuscript, we examine the production of glycogen in the model cyanobacteria, Synechococcus sp. strain PCC 7002, and subsequent conversion of cyanobacterial biomass by an engineered Escherichia coli to octanoic acid as a model product. In effort to maximize glycogen production, we explored the deletion of catabolic enzymes and overexpression of GlgC, an enzyme that catalyzes the first committed step toward glycogen synthesis. We found that deletion of glgP increased final glycogen titers when cells were grown in diurnal light. Overexpression of GlgC led to a temporal increase in glycogen content but not in an overall increase in final titer or content. The best strains were grown, harvested, and used to formulate media for growth of E. coli. The cyanobacterial media wasmore » able to support the growth of an engineered E. coli and produce octanoic acid at the same titer as common laboratory media.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Vanderbilt Univ., Nashville, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Science Foundation (NSF)
OSTI Identifier:
1631188
Alternate Identifier(s):
OSTI ID: 1633984
Grant/Contract Number:  
SC0019404; EFRI-1240268; GEO-1215871
Resource Type:
Published Article
Journal Name:
Frontiers in Energy Research
Additional Journal Information:
Journal Name: Frontiers in Energy Research Journal Volume: 8; Journal ID: ISSN 2296-598X
Publisher:
Frontiers Research Foundation
Country of Publication:
Switzerland
Language:
English
Subject:
09 BIOMASS FUELS; cyanobacteria; glycogen; metabolic engineering; biofuels; feedstock

Citation Formats

Comer, Austin D., Abraham, Joshua P., Steiner, Alexander J., Korosh, Travis C., Markley, Andrew L., and Pfleger, Brian F. Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock. Switzerland: N. p., 2020. Web. doi:10.3389/fenrg.2020.00093.
Comer, Austin D., Abraham, Joshua P., Steiner, Alexander J., Korosh, Travis C., Markley, Andrew L., & Pfleger, Brian F. Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock. Switzerland. https://doi.org/10.3389/fenrg.2020.00093
Comer, Austin D., Abraham, Joshua P., Steiner, Alexander J., Korosh, Travis C., Markley, Andrew L., and Pfleger, Brian F. Fri . "Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock". Switzerland. https://doi.org/10.3389/fenrg.2020.00093.
@article{osti_1631188,
title = {Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock},
author = {Comer, Austin D. and Abraham, Joshua P. and Steiner, Alexander J. and Korosh, Travis C. and Markley, Andrew L. and Pfleger, Brian F.},
abstractNote = {Current sources of fermentation feedstocks, i.e., corn, sugar cane, or plant biomass, fall short of demand for liquid transportation fuels and commodity chemicals in the United States. Aquatic phototrophs including cyanobacteria have the potential to supplement the supply of current fermentable feedstocks. In our strategy, cells are engineered to accumulate storage molecules including glycogen, cellulose, and/or lipid oils that can be extracted from harvested biomass and fed to heterotrophic organisms engineered to produce desired chemical products. Here in this manuscript, we examine the production of glycogen in the model cyanobacteria, Synechococcus sp. strain PCC 7002, and subsequent conversion of cyanobacterial biomass by an engineered Escherichia coli to octanoic acid as a model product. In effort to maximize glycogen production, we explored the deletion of catabolic enzymes and overexpression of GlgC, an enzyme that catalyzes the first committed step toward glycogen synthesis. We found that deletion of glgP increased final glycogen titers when cells were grown in diurnal light. Overexpression of GlgC led to a temporal increase in glycogen content but not in an overall increase in final titer or content. The best strains were grown, harvested, and used to formulate media for growth of E. coli. The cyanobacterial media was able to support the growth of an engineered E. coli and produce octanoic acid at the same titer as common laboratory media.},
doi = {10.3389/fenrg.2020.00093},
journal = {Frontiers in Energy Research},
number = ,
volume = 8,
place = {Switzerland},
year = {2020},
month = {5}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.3389/fenrg.2020.00093

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