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Title: Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs

Abstract

Abstract Background Glaesserella parasuis , the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity. Subunit vaccines are of interest to provide protective immunity to multiple strains of G. parasuis . Selected proteins for subunit vaccination should be widespread, highly conserved, and surface exposed. Results Two candidate proteins for subunit vaccination (RlpB and VacJ) against G. parasuis were identified using random mutagenesis and an in vitro organ culture system. Pigs were vaccinated with recombinant RlpB and VacJ, outer membrane proteins with important contributions to cellular function and viability. Though high antibody titers to the recombinant proteins and increased interferon-γ producing cells were found in subunit vaccinated animals, the pigs were not protected from developing systemic disease. Conclusions It appears there may be insufficient RlpB and VacJ exposed on the bacterial surface for antibody to bind, preventing high RlpB and VacJ specific antibody titers from protecting animals from G. parasuis . Additionally, this work confirms the importance of utilizing the natural host speciesmore » when assessing the efficacy of vaccine candidates.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1630982
Resource Type:
Published Article
Journal Name:
BMC Veterinary Research
Additional Journal Information:
Journal Name: BMC Veterinary Research Journal Volume: 16 Journal Issue: 1; Journal ID: ISSN 1746-6148
Publisher:
Springer Science + Business Media
Country of Publication:
United Kingdom
Language:
English

Citation Formats

on behalf of the BRaDP1T Consortium, Hau, Samantha J., Luan, Shi-Lu, Loving, Crystal L., Nicholson, Tracy L., Wang, Jinhong, Peters, Sarah E., Seilly, David, Weinert, Lucy A., Langford, Paul R., Rycroft, Andrew N., Wren, Brendan W., Maskell, Duncan J., Tucker, Alexander W., and Brockmeier, Susan L. Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs. United Kingdom: N. p., 2020. Web. doi:10.1186/s12917-020-02377-5.
on behalf of the BRaDP1T Consortium, Hau, Samantha J., Luan, Shi-Lu, Loving, Crystal L., Nicholson, Tracy L., Wang, Jinhong, Peters, Sarah E., Seilly, David, Weinert, Lucy A., Langford, Paul R., Rycroft, Andrew N., Wren, Brendan W., Maskell, Duncan J., Tucker, Alexander W., & Brockmeier, Susan L. Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs. United Kingdom. https://doi.org/10.1186/s12917-020-02377-5
on behalf of the BRaDP1T Consortium, Hau, Samantha J., Luan, Shi-Lu, Loving, Crystal L., Nicholson, Tracy L., Wang, Jinhong, Peters, Sarah E., Seilly, David, Weinert, Lucy A., Langford, Paul R., Rycroft, Andrew N., Wren, Brendan W., Maskell, Duncan J., Tucker, Alexander W., and Brockmeier, Susan L. Wed . "Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs". United Kingdom. https://doi.org/10.1186/s12917-020-02377-5.
@article{osti_1630982,
title = {Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs},
author = {on behalf of the BRaDP1T Consortium and Hau, Samantha J. and Luan, Shi-Lu and Loving, Crystal L. and Nicholson, Tracy L. and Wang, Jinhong and Peters, Sarah E. and Seilly, David and Weinert, Lucy A. and Langford, Paul R. and Rycroft, Andrew N. and Wren, Brendan W. and Maskell, Duncan J. and Tucker, Alexander W. and Brockmeier, Susan L.},
abstractNote = {Abstract Background Glaesserella parasuis , the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity. Subunit vaccines are of interest to provide protective immunity to multiple strains of G. parasuis . Selected proteins for subunit vaccination should be widespread, highly conserved, and surface exposed. Results Two candidate proteins for subunit vaccination (RlpB and VacJ) against G. parasuis were identified using random mutagenesis and an in vitro organ culture system. Pigs were vaccinated with recombinant RlpB and VacJ, outer membrane proteins with important contributions to cellular function and viability. Though high antibody titers to the recombinant proteins and increased interferon-γ producing cells were found in subunit vaccinated animals, the pigs were not protected from developing systemic disease. Conclusions It appears there may be insufficient RlpB and VacJ exposed on the bacterial surface for antibody to bind, preventing high RlpB and VacJ specific antibody titers from protecting animals from G. parasuis . Additionally, this work confirms the importance of utilizing the natural host species when assessing the efficacy of vaccine candidates.},
doi = {10.1186/s12917-020-02377-5},
journal = {BMC Veterinary Research},
number = 1,
volume = 16,
place = {United Kingdom},
year = {2020},
month = {5}
}

Works referenced in this record:

Virulence, Transmission, and Heterologous Protection of Four Isolates of Haemophilus parasuis
journal, July 2013

  • Brockmeier, Susan L.; Loving, Crystal L.; Mullins, Michael A.
  • Clinical and Vaccine Immunology, Vol. 20, Issue 9
  • DOI: 10.1128/CVI.00168-13

Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules
journal, July 2014

  • Cress, Brady F.; Englaender, Jacob A.; He, Wenqin
  • FEMS Microbiology Reviews, Vol. 38, Issue 4
  • DOI: 10.1111/1574-6976.12056

Molecular Basis of Increased Serum Resistance among Pulmonary Isolates of Non-typeable Haemophilus influenzae
journal, January 2011


The use of genome wide association methods to investigate pathogenicity, population structure and serovar in Haemophilus parasuis
journal, January 2014


Protection of vaccinated pigs against experimental infections with homologous and heterologous Hoemophilus parasuis
journal, October 2002


Designation of 15 serovars of Haemophilus parasuis on the basis of immunodiffusion using heat-stable antigen extracts.
journal, January 1992


Characterization of the diversity of Haemophilus parasuis field isolates by use of serotyping and genotyping
journal, April 2003

  • Oliveira, Simone; Blackall, P. J.; Pijoan, Carlos
  • American Journal of Veterinary Research, Vol. 64, Issue 4
  • DOI: 10.2460/ajvr.2003.64.435

Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis
journal, August 2014


Genome sequence of Haemophilus parasuis strain 29755
journal, September 2011

  • Mullins, Michael A.; Register, Karen B.; Bayles, Darrell O.
  • Standards in Genomic Sciences, Vol. 5, Issue 1
  • DOI: 10.4056/sigs.2245029

Haemophilus parasuis vaccines
journal, November 2016


A Cross-Protection Experiment in Pigs Vaccinated with Haemophilus parasuis Serovars 2 and 5 Bacterins, and Evaluation of a Bivalent Vaccine under Laboratory and Field Conditions.
journal, January 2001

  • Takahashi, Kinya; Nagai, Shinya; Yagihashi, Takeshi
  • Journal of Veterinary Medical Science, Vol. 63, Issue 5
  • DOI: 10.1292/jvms.63.487

Functional Analysis of the Protein Machinery Required for Transport of Lipopolysaccharide to the Outer Membrane of Escherichia coli
journal, April 2008

  • Sperandeo, P.; Lau, F. K.; Carpentieri, A.
  • Journal of Bacteriology, Vol. 190, Issue 13
  • DOI: 10.1128/JB.00270-08

The amino acid selected for generating mutant TbpB antigens defective in binding transferrin can compromise the in vivo protective capacity
journal, May 2018

  • Guizzo, João Antônio; Chaudhuri, Somshukla; Prigol, Simone Ramos
  • Scientific Reports, Vol. 8, Issue 1
  • DOI: 10.1038/s41598-018-25685-1

Identification of secreted proteins as novel antigenic vaccine candidates of Haemophilus parasuis serovar 5
journal, March 2015


Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis
journal, March 2016


Serovar profiling of Haemophilus parasuis on Australian farms by sampling live pigs: PRODUCTION ANIMALS
journal, June 2010


Deletion of the vacJ gene affects the biology and virulence in Haemophilus parasuis serovar 5
journal, March 2017


Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli
journal, July 2006

  • Wu, T.; McCandlish, A. C.; Gronenberg, L. S.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 31
  • DOI: 10.1073/pnas.0604744103

Identification and characterization of novel immunogenic outer membrane proteins of Haemophilus parasuis serovar 5
journal, August 2009


Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome.
journal, January 1987