Association between algal productivity and phycosphere composition in an outdoor Chlorella sorokiniana reactor based on multiple longitudinal analyses

Steichen, Seth A.; Gao, Song; Waller, Peter; Brown, Judith K.

doi:10.1111/1751-7915.13591

Title: Association between algal productivity and phycosphere composition in an outdoor Chlorella sorokiniana reactor based on multiple longitudinal analyses

Journal Article · 25 May 2020 · Microbial Biotechnology (Online)

DOI: https://doi.org/10.1111/1751-7915.13591 · OSTI ID:1630683

^[1]; Gao, Song ^[2]; Waller, Peter ^[3]; Brown, Judith K. ^[1]

School of Plant Sciences The University of Arizona 1140 E South Campus Dr Tucson AZ 85721 USA
Pacific Northwest National Laboratory 1529 West Sequim Bay Road Sequim WA 98382 USA
Biosystems Engineering The University of Arizona 1177 E 4th St Tucson AZ 85721 USA

Summary Microalgae as a biofuel source are of great interest. Bacterial phycosphere inhabitants of algal cultures are hypothesized to contribute to productivity. In this study, the bacterial composition of the Chlorella sorokiniana phycosphere was determined over several production cycles in different growing seasons by 16S rRNA gene sequencing and identification. The diversity of the phycosphere increased with time during each individual reactor run, based on Faith’s phylogenetic diversity metric versus days post‐inoculation ( R = 0.66, P < 0.001). During summer months, Vampirovibrio chlorellavorus , an obligate predatory bacterium, was prevalent. Bacterial sequences assigned to the Rhizobiales, Betaproteobacteriales and Chitinophagales were positively associated with algal biomass productivity. Applications of the general biocide, benzalkonium chloride, to a subset of experiments intended to abate V. chlorellavorus appeared to temporarily suppress phycosphere bacterial growth, however, there was no relationship between those bacterial taxa suppressed by benzalkonium chloride and their association with algal productivity, based on multinomial model correlations. Algal health was approximated using a model‐based metric, or the ‘Health Index’ that indicated a robust, positive relationship between C. sorokiniana fitness and presence of members belonging to the Burholderiaceae and Allorhizobium–Neorhizobium–Pararhizobium–Rhizobium clade. Bacterial community composition was linked to the efficiency of microalgal biomass production and algal health.

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Research Organization:: Battelle Memorial Inst., Columbus, OH (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC05-76RL01830; EE0006269

OSTI ID:: 1630683

Journal Information:: Microbial Biotechnology (Online), Journal Name: Microbial Biotechnology (Online) Journal Issue: 5 Vol. 13; ISSN 1751-7915

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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