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Title: Acclimatory responses of the Daphnia pulex proteome to environmental changes. I. Chronic exposure to hypoxia affects the oxygen transport system and carbohydrate metabolism

Journal Article · · BMC Physiology
 [1];  [2];  [3];  [3];  [3];  [3];  [2];  [2];  [2];  [3]
  1. Univ. of Munster (Germany). Inst. of Zoophysiology; DOE/OSTI
  2. Univ. of Tubingen (Germany). Interfaculty Inst. for Cell Biology. Proteom Centrum Tubingen
  3. Univ. of Munster (Germany). Inst. of Zoophysiology
+ Show Author Affiliations

Background: Freshwater planktonic crustaceans of the genus Daphnia show a remarkable plasticity to cope with environmental changes in oxygen concentration and temperature. One of the key proteins of adaptive gene control in Daphnia pulex under hypoxia is hemoglobin (Hb), which increases in hemolymph concentration by an order of magnitude and shows an enhanced oxygen affinity due to changes in subunit composition. To explore the full spectrum of adaptive protein expression in response to low-oxygen conditions, two-dimensional gel electrophoresis and mass spectrometry were used to analyze the proteome composition of animals acclimated to normoxia (oxygen partial pressure [Po2]: 20 kPa) and hypoxia (Po2: 3 kPa), respectively. Results: The comparative proteome analysis showed an up-regulation of more than 50 protein spots under hypoxia. Identification of a major share of these spots revealed acclimatory changes for Hb, glycolytic enzymes (enolase), and enzymes involved in the degradation of storage and structural carbohydrates (e.g. cellubiohydrolase). Proteolytic enzymes remained constitutively expressed on a high level. Conclusion: Acclimatory adjustments of the D. pulex proteome to hypoxia included a strong induction of Hb and carbohydrate-degrading enzymes. The scenario of adaptive protein expression under environmental hypoxia can be interpreted as a process to improve oxygen transport and carbohydrate provision for the maintenance of ATP production, even during short episodes of tissue hypoxia requiring support from anaerobic metabolism.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231; W-7405-ENG-36
OSTI ID:
1629743
Journal Information:
BMC Physiology, Journal Name: BMC Physiology Journal Issue: 1 Vol. 9; ISSN 1472-6793
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Low Genotypic Diversity in a Daphnia Pulex Population in a Biomanipulated Lake: The Lack of Vertical and Seasonal Variability journal September 2004
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  • Proceedings of the Royal Society of London. Series B - Biological Sciences, Vol. 136, Issue 884, p. 388-399 https://doi.org/10.1098/rspb.1949.0032
journal October 1949
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HIF-1 and human disease: one highly involved factor journal August 2000
Control of oxygen transport in the microcrustacean Daphnia: regulation of haemoglobin expression as central mechanism of adaptation to different oxygen and temperature conditions journal November 2004
A comparative study of cellulase and xylanase activity in freshwater crayfish and marine prawns journal April 2005
The digestion of cellulose and other dietary components, and pH of the gut in the amphipod Gammarus pulex (L.) journal October 1977
Implications of the three-dimensional structure of astacin for the structure and function of the astacin family of zinc-endopeptidases journal May 1993
Molecular mass of macromolecules and subunits and the quaternary structure of hemoglobin from the microcrustacean Daphnia magna journal July 2006
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Temperature acclimation influences temperature-related behaviour as well as oxygen-transport physiology and biochemistry in the water flea Daphnia magna journal February 2003
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Antioxidant capacity, lipid peroxidation, and lipid composition changes during long-term and short-term thermal acclimation in Daphnia journal April 2017
Linking genes to communities and ecosystems: Daphnia as an ecogenomic model journal February 2012
Adaptive phenotypic plasticity and local adaptation for temperature tolerance in freshwater zooplankton journal February 2014
Haemoglobin-mediated response to hyper-thermal stress in the keystone species Daphnia magna journal November 2017
The combined effects of hypoxia and fish kairomones on several physiological and life history traits of Daphnia journal September 2019
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