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Title: Regulation and Maturation of the Shewanella oneidensis Sulfite Reductase SirA

Journal Article · · Scientific Reports
ORCiD logo [1];  [2];  [2];  [2];  [2]
  1. Univ. of Wisconsin, Milwaukee, WI (United States). Dept. of Biological Sciences; DOE/OSTI
  2. Univ. of Wisconsin, Milwaukee, WI (United States). Dept. of Biological Sciences
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Shewanella oneidensis, a metal reducer and facultative anaerobe, expresses a large number of c-type cytochromes, many of which function as anaerobic reductases. All of these proteins contain the typical heme-binding motif CXXCH and require the Ccm proteins for maturation. Two c-type cytochrome reductases also possess atypical heme-binding sites, the NrfA nitrite reductase (CXXCK) and the SirA sulfte reductase (CX12NKGCH). S. oneidensis MR-1 encodes two cytochrome c synthetases (CcmF and SirE) and two apocytochrome c chaperones (CcmI and SirG). SirE located in the sir gene cluster is required for the maturation of SirA, but not NrfA. Here we show that maturation of SirA requires the combined function of the two apocytochrome c chaperones CcmI and SirG. Loss of either protein resulted in decreased sulfte reductase. Furthermore, SirA was not detected in a mutant that lacked both chaperones, perhaps due to misfolding or instability. These results suggest that CcmI interacts with SirEFG during SirA maturation, and with CcmF during maturation of NrfA. Additionally, we show that CRP regulates expression of sirA via the newly identifed transcriptional regulatory protein, SirR.

Research Organization:
Univ. of Wisconsin, Milwaukee, WI (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
FG02-07ER64382
OSTI ID:
1629707
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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The nrfI gene is essential for the attachment of the active site haem group of Wolinella succinogenes cytochrome c nitrite reductase: Maturation of W. succinogenes nitrite reductase journal February 2002
Extracellular respiration of dimethyl sulfoxide by Shewanella oneidensis strain MR-1 journal March 2006
From one gene to two proteins: the biogenesis of cytochromes b and c1 in Bradyrhizobium japonicum. journal June 1991
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CcmI Subunit of CcmFHI Heme Ligation Complex Functions as an Apocytochrome c Chaperone during c -Type Cytochrome Maturation journal September 2011
Structural Basis of Redox-coupled Protein Substrate Selection by the Cytochrome c Biosynthesis Protein ResA journal May 2004
The Cytochrome c Maturation Components CcmF, CcmH, and CcmI Form a Membrane-integral Multisubunit Heme Ligation Complex journal August 2008
Identification of 42 Possible Cytochrome C Genes in the Shewanella oneidensis Genome and Characterization of Six Soluble Cytochromes journal January 2004
Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp. nov. journal April 1999
Dissimilatory sulphite reductase from Archaeoglobus fulgidus: physico-chemical properties of the enzyme and cloning, sequencing and analysis of the reductase genes journal August 1993
A seven-gene operon essential for formate-dependent nitrite reduction to ammonia by enteric bacteria journal April 1994
Substrate specificity of three cytochrome c haem lyase isoenzymes from Wolinella succinogenes : unconventional haem c binding motifs are not sufficient for haem c attachment by NrfI and CcsA1 journal January 2010
The Wolinella succinogenes mcc gene cluster encodes an unconventional respiratory sulphite reduction system: The respiratory Mcc sulphite reduction system journal November 2011
Hydrogenase- and outer membrane c-type cytochrome-facilitated reduction of technetium(VII) by Shewanella oneidensis MR-1 journal September 2007
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Identification of Genes Involved in Cytochrome c Biogenesis in Shewanella oneidensis, Using a Modified mariner Transposon journal August 2005
Respiratory Nitrate Ammonification by Shewanella oneidensis MR-1 journal November 2006
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Involvement of a Membrane-Bound Class III Adenylate Cyclase in Regulation of Anaerobic Respiration in Shewanella oneidensis MR-1 journal April 2009
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c-Type Cytochrome-Dependent Formation of U(IV) Nanoparticles by Shewanella oneidensis journal August 2006
Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis journal September 2013
Spectrophotometric Determination Of Hydrogen Sulfide In Natural Waters journal May 1969

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