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Title: Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine

Journal Article · · Scientific Reports
 [1];  [2];  [2];  [3];  [3];  [4];  [5];  [6];  [6];  [7];  [7]; ORCiD logo [2]
  1. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); DOE/OSTI
  2. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS)
  3. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Univ. of Connecticut, Storrs, CT (United States). Dept. of Pathobiology and Veterinary Science
  4. Mississippi State Univ., Mississippi State, MS (United States). Dept. of Pathology and Population Medicine
  5. Kansas State Univ., Manhattan, KS (United States). Dept. of Anatomy and Physiology
  6. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
  7. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Kansas State Univ., Manhattan, KS (United States). Dept. of Anatomy and Physiology
+ Show Author Affiliations

The CD2-like African swine fever virus (ASFV) gene 8DR, (also known as EP402R) encodes for a structural transmembrane glycoprotein that has been shown to mediate hemadsorption and be involved in host immunomodulation as well as the induction of protective immune response. In addition, several natural ASFV isolates showing decreased virulence in swine has been shown to be non-hemadsorbing suggesting an association between altered or deleted forms of 8DR and virus attenuation. Here we demonstrate that deletion of 8DR gene from the genome of ASFV Georgia2010 isolate (ASFV-G-Δ8DR) does not significantly alter the virulence of the virus. ASFV-G-Δ8DR inoculated intramuscularly or intranasally (in a range of 102 to 104TCID50) produced a clinical disease in domestic pigs indistinguishable from that induced by the same doses of the virulent parental ASFV Georgia2010 isolate. In addition, viremia values in ASFV-G-Δ8DR do not differ from those detected in animals infected with parental virus. Therefore, deletion of 8DR gene is not associated with a noticeable decrease in virulence of the ASFV Georgia isolate.

Research Organization:
Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
SC0014664
OSTI ID:
1629662
Journal Information:
Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge journal August 2016
The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β journal April 2018
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African swine fever: how can global spread be prevented?
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African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever journal July 2016
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Attenuated and non‐haemadsorbing (non‐ HAD ) genotype II African swine fever virus ( ASFV ) isolated in Europe, Latvia 2017 journal February 2019
African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus journal March 2015
The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome journal December 2014
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Genomic Analysis of Highly Virulent Georgia 2007/1 Isolate of African Swine Fever Virus journal April 2011
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The CD2v protein enhances African swine fever virus replication in the tick vector, Ornithodoros erraticus journal October 2009
African swine fever virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes is highly attenuated in swine but does not confer protection against parental virus challenge journal August 2016
The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β journal April 2018
CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses journal February 2018
Development of a fluorescent ASFV strain that retains the ability to cause disease in swine journal April 2017
African swine fever: how can global spread be prevented?
  • Costard, Solenne; Wieland, Barbara; de Glanville, William
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 364, Issue 1530 https://doi.org/10.1098/rstb.2009.0098
journal September 2009
African swine fever virus NL gene is not required for virus virulence. journal October 1998
The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response journal March 2001
African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever journal July 2016
Related strains of African swine fever virus with different virulence: genome comparison and analysis journal February 2015
Characterization of pathogenic and non-pathogenic African swine fever virus isolates from Ornithodoros erraticus inhabiting pig premises in Portugal journal August 2004
Comparison of the genome sequences of non-pathogenic and pathogenic African swine fever virus isolates journal February 2008
Attenuated and non‐haemadsorbing (non‐ HAD ) genotype II African swine fever virus ( ASFV ) isolated in Europe, Latvia 2017 journal February 2019
African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus journal March 2015
African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge journal June 2015
BA71ΔCD2: a New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities journal August 2017
The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome journal December 2014
African swine fever virus encodes a CD2 homolog responsible for the adhesion of erythrocytes to infected cells. journal January 1993
An African swine fever virus virulence-associated gene NL-S with similarity to the herpes simplex virus ICP34.5 gene. journal January 1996
A Nonessential African Swine Fever Virus Gene UK Is a Significant Virulence Determinant in Domestic Swine journal February 1998
Deletion of a CD2-Like Gene, 8-DR, from African Swine Fever Virus Affects Viral Infection in Domestic Swine journal April 1998
An African Swine Fever Virus ERV1-ALRHomologue, 9GL, Affects Virion Maturation and Viral Growth in Macrophages and Viral Virulence in Swine journal February 2000
Genomic Analysis of Highly Virulent Georgia 2007/1 Isolate of African Swine Fever Virus journal April 2011
Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain journal July 2019

Cited By (2)

Additional file 1 of The CD2v protein of African swine fever virus inhibits macrophage migration and inflammatory cytokines expression by downregulating EGR1 expression through dampening ERK1/2 activity image January 2023
A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs journal March 2020

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