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Title: Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine

Abstract

The CD2-like African swine fever virus (ASFV) gene 8DR, (also known as EP402R) encodes for a structural transmembrane glycoprotein that has been shown to mediate hemadsorption and be involved in host immunomodulation as well as the induction of protective immune response. In addition, several natural ASFV isolates showing decreased virulence in swine has been shown to be non-hemadsorbing suggesting an association between altered or deleted forms of 8DR and virus attenuation. Here we demonstrate that deletion of 8DR gene from the genome of ASFV Georgia2010 isolate (ASFV-G-Δ8DR) does not significantly alter the virulence of the virus. ASFV-G-Δ8DR inoculated intramuscularly or intranasally (in a range of 102 to 104TCID50) produced a clinical disease in domestic pigs indistinguishable from that induced by the same doses of the virulent parental ASFV Georgia2010 isolate. In addition, viremia values in ASFV-G-Δ8DR do not differ from those detected in animals infected with parental virus. Therefore, deletion of 8DR gene is not associated with a noticeable decrease in virulence of the ASFV Georgia isolate.

Authors:
 [1];  [1];  [1];  [2];  [2];  [3];  [4];  [5];  [5];  [6];  [7]; ORCiD logo [1]
+ Show Author Affiliations
  1. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS)
  2. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Univ. of Connecticut, Storrs, CT (United States). Dept. of Pathobiology and Veterinary Science
  3. Mississippi State Univ., Mississippi State, MS (United States). Dept. of Pathology and Population Medicine
  4. Kansas State Univ., Manhattan, KS (United States). Dept. of Anatomy and Physiology
  5. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
  6. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Kansas State Univ., Manhattan, KS (United States). Dept. of Anatomy and Physiology
  7. US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center. Agricultural Research Service (ARS); Kansas State Univ., Manhattan, KS (United States). Dept. of Anatomy and Physiology
Publication Date:
Research Org.:
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
OSTI Identifier:
1629662
Grant/Contract Number:  
SC0014664
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Borca, Manuel V., O’Donnell, Vivian, Holinka, Lauren G., Risatti, Guillermo R., Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Shi, Jishu, Pruitt, Sarah, Rai, Ayushi, Silva, Ediane, Velazquez-Salinas, Lauro, and Gladue, Douglas P. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. United States: N. p., 2020. Web. doi:10.1038/s41598-020-57455-3.
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Borca, Manuel V., O’Donnell, Vivian, Holinka, Lauren G., Risatti, Guillermo R., Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Shi, Jishu, Pruitt, Sarah, Rai, Ayushi, Silva, Ediane, Velazquez-Salinas, Lauro, & Gladue, Douglas P. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. United States. https://doi.org/10.1038/s41598-020-57455-3
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Borca, Manuel V., O’Donnell, Vivian, Holinka, Lauren G., Risatti, Guillermo R., Ramirez-Medina, Elizabeth, Vuono, Elizabeth A., Shi, Jishu, Pruitt, Sarah, Rai, Ayushi, Silva, Ediane, Velazquez-Salinas, Lauro, and Gladue, Douglas P. Thu . "Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine". United States. https://doi.org/10.1038/s41598-020-57455-3. https://www.osti.gov/servlets/purl/1629662.
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@article{osti_1629662,
title = {Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine},
author = {Borca, Manuel V. and O’Donnell, Vivian and Holinka, Lauren G. and Risatti, Guillermo R. and Ramirez-Medina, Elizabeth and Vuono, Elizabeth A. and Shi, Jishu and Pruitt, Sarah and Rai, Ayushi and Silva, Ediane and Velazquez-Salinas, Lauro and Gladue, Douglas P.},
abstractNote = {The CD2-like African swine fever virus (ASFV) gene 8DR, (also known as EP402R) encodes for a structural transmembrane glycoprotein that has been shown to mediate hemadsorption and be involved in host immunomodulation as well as the induction of protective immune response. In addition, several natural ASFV isolates showing decreased virulence in swine has been shown to be non-hemadsorbing suggesting an association between altered or deleted forms of 8DR and virus attenuation. Here we demonstrate that deletion of 8DR gene from the genome of ASFV Georgia2010 isolate (ASFV-G-Δ8DR) does not significantly alter the virulence of the virus. ASFV-G-Δ8DR inoculated intramuscularly or intranasally (in a range of 102 to 104TCID50) produced a clinical disease in domestic pigs indistinguishable from that induced by the same doses of the virulent parental ASFV Georgia2010 isolate. In addition, viremia values in ASFV-G-Δ8DR do not differ from those detected in animals infected with parental virus. Therefore, deletion of 8DR gene is not associated with a noticeable decrease in virulence of the ASFV Georgia isolate.},
doi = {10.1038/s41598-020-57455-3},
journal = {Scientific Reports},
number = 1,
volume = 10,
place = {United States},
year = {Thu Jan 16 00:00:00 EST 2020},
month = {Thu Jan 16 00:00:00 EST 2020}
}
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https://doi.org/10.1038/s41598-020-57455-3
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Works referenced in this record:

Genomic Analysis of Highly Virulent Georgia 2007/1 Isolate of African Swine Fever Virus
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African swine fever virus NL gene is not required for virus virulence.
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Differential Effect of the Deletion of African Swine Fever Virus Virulence-Associated Genes in the Induction of Attenuation of the Highly Virulent Georgia Strain
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A Nonessential African Swine Fever Virus Gene UK Is a Significant Virulence Determinant in Domestic Swine
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BA71ΔCD2: a New Recombinant Live Attenuated African Swine Fever Virus with Cross-Protective Capabilities
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CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses
journal, February 2018

The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β
journal, April 2018

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A Conserved African Swine Fever Virus IκB Homolog, 5EL, Is Nonessential for Growthin Vitroand Virulence in Domestic Swine
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Functional and Immunological Properties of the Baculovirus-Expressed Hemagglutinin of African Swine Fever Virus
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A Conserved African Swine Fever Virus IκB Homolog, 5EL, Is Nonessential for Growthin Vitroand Virulence in Domestic Swine
journal, September 1997

The CD2v protein enhances African swine fever virus replication in the tick vector, Ornithodoros erraticus
journal, October 2009

The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β
journal, April 2018

CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses
journal, February 2018

African swine fever: how can global spread be prevented?
journal, September 2009

  • Costard, Solenne; Wieland, Barbara; de Glanville, William
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 364, Issue 1530
  • DOI: 10.1098/rstb.2009.0098

African swine fever virus NL gene is not required for virus virulence.
journal, October 1998

African swine fever virus serotype-specific proteins are significant protective antigens for African swine fever
journal, July 2016

  • Burmakina, G.; Malogolovkin, A.; Tulman, E. R.
  • Journal of General Virology, Vol. 97, Issue 7
  • DOI: 10.1099/jgv.0.000490

Attenuated and non‐haemadsorbing (non‐ HAD ) genotype II African swine fever virus ( ASFV ) isolated in Europe, Latvia 2017
journal, February 2019

  • Gallardo, Carmina; Soler, Alejandro; Rodze, Ieva
  • Transboundary and Emerging Diseases, Vol. 66, Issue 3
  • DOI: 10.1111/tbed.13132

African swine fever virus encodes a CD2 homolog responsible for the adhesion of erythrocytes to infected cells.
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journal, January 1996

Deletion of a CD2-Like Gene, 8-DR, from African Swine Fever Virus Affects Viral Infection in Domestic Swine
journal, April 1998

Genomic Analysis of Highly Virulent Georgia 2007/1 Isolate of African Swine Fever Virus
journal, April 2011

  • Chapman, David A. G.; Darby, Alistair C.; Da Silva, Melissa
  • Emerging Infectious Diseases, Vol. 17, Issue 4
  • DOI: 10.3201/eid1704.101283
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    A seven-gene-deleted African swine fever virus is safe and effective as a live attenuated vaccine in pigs
    journal, March 2020

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