Populus resequencing: towards genome-wide association studies

Journal Article · 13 September 2011 · BMC Proceedings

DOI: https://doi.org/10.1186/1753-6561-5-S7-I21 · OSTI ID:1628947

Tuskan, Gerald ^[1]; Slavov, Gancho ^[2]; DiFazio, Steve ^[2]; Muchero, Wellington ^[1]; Pryia, Ranjan ^[1]; Schackwitz, Wendy ^[3]; Martin, Joel ^[3]; Rokhsar, Daniel ^[3]; Sykes, Robert ^[4]; Davis, Mark ^[4]; Studer, Michael ^[5]; Wyman, Charles ^[5]

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1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
2. West Virginia Univ., Morgantown, WV (United States)
3. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
4. National Renewable Energy Lab. (NREL), Golden, CO (United States)
5. Univ. of California, Riverside, CA (United States)

Genome-wide association studies (GWAS) have been used to identify regions of the genome related to various phenotypes in humans, corn, rice and cattle. Successful application of this approach to bioenergy crops such as Populus requires 1) an appropriate mapping population, 2) high-quality phenotypic data and 3) informative genotypic data. With the goal of reducing the recalcitrance of lignocellulosic biomass for economic production of biofuels and understanding basic mechanisms of cell wall formation in Populus we established 4 clonally replicated common gardens experiments each with 1100 native P. trichocarpa genotypes collected from along the northwest coast of the U.S. and Canada.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
• Grant/Contract Number: AC05-00OR22725
• OSTI ID: 1628947
• Journal Information: BMC Proceedings, Vol. 5, Issue S7; ISSN 1753-6561
• Publisher: BioMed CentralCopyright Statement
• Country of Publication: United States
• Language: English