Title: Efficient Eucalypt Cell Wall Deconstruction and Conversion for Sustainable Lignocellulosic Biofuels

Journal Article · · Frontiers in Bioengineering and Biotechnology
 [1];  [2];  [3];  [4];  [3]
  1. Univ. of Queensland, Brisbane, QLD (Australia). Queensland Alliance for Agriculture and Food Innovation; DOE/OSTI
  2. Univ. of the Sunshine Coast, QLD (Australia). Forest Industries Research Centre; Agri-Science Queensland, QLD (Australia). Dept. of Agriculture and Fisheries, Forestry and Biosciences
  3. Univ. of Queensland, Brisbane, QLD (Australia). Queensland Alliance for Agriculture and Food Innovation
  4. Univ. of Queensland, Brisbane, QLD (Australia). Queensland Alliance for Agriculture and Food Innovation; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States). Biological and Engineering Sciences Center

In order to meet the world’s growing energy demand and reduce the impact of greenhouse gas emissions resulting from fossil fuel combustion, renewable plant-based feedstocks for biofuel production must be considered. The first-generation biofuels, derived from starches of edible feedstocks, such as corn, create competition between food and fuel resources, both for the crop itself and the land on which it is grown. As such, biofuel synthesized from non-edible plant biomass (lignocellulose) generated on marginal agricultural land will help to alleviate this competition. Eucalypts, the broadly defined taxa encompassing over 900 species of Eucalyptus, Corymbia, and Angophora are the most widely planted hardwood tree in the world, harvested mainly for timber, pulp and paper, and biomaterial products. More recently, due to their exceptional growth rate and amenability to grow under a wide range of environmental conditions, eucalypts are a leading option for the development of a sustainable lignocellulosic biofuels. However, efficient conversion of woody biomass into fermentable monomeric sugars is largely dependent on pretreatment of the cell wall, whose formation and complexity lend itself toward natural recalcitrance against its efficient deconstruction. A greater understanding of this complexity within the context of various pretreatments will allow the design of new and effective deconstruction processes for bioenergy production. In this review, we present the various pretreatment options for eucalypts, including research into understanding structure and formation of the eucalypt cell wall.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Sandia National Laboratories (SNL-CA), Livermore, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1628943
Journal Information:
Frontiers in Bioengineering and Biotechnology, Journal Name: Frontiers in Bioengineering and Biotechnology Vol. 3; ISSN 2296-4185
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

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EgMYB1, an R2R3 MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar journal October 2010
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Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus journal January 2008
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Bioconversion of lignocellulose: inhibitors and detoxification journal January 2013
Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production – part 1 journal January 2013
Pretreatment with laccase and a phenolic mediator degrades lignin and enhances saccharification of Eucalyptus feedstock journal January 2014
Next-generation biomass feedstocks for biofuel production journal January 2008
Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release journal August 2015
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Understanding the impact of ionic liquid pretreatment on eucalyptus journal January 2010
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  • European Journal of Applied Microbiology and Biotechnology, Vol. 18, Issue 5 https://doi.org/10.1007/BF00500493
journal January 1983
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Bioethanol production from tension and opposite wood of Eucalyptus globulus using organosolv pretreatment and simultaneous saccharification and fermentation journal April 2011
Genomic Research in Eucalyptus journal September 2005
Genetic modification of lignin biosynthesis for improved biofuel production journal May 2009
Short-rotation woody crops for bioenergy and biofuels applications journal August 2009
Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process journal October 2010
Study on the Decreased Sugar Yield in Enzymatic Hydrolysis of Cellulosic Substrate at High Solid Loading journal February 2011
In-Depth 2D NMR Study of Lignin Modification During Pretreatment of Eucalyptus Wood with Laccase and Mediators journal August 2014
Impact of Pretreatment Technologies on Saccharification and Isopentenol Fermentation of Mixed Lignocellulosic Feedstocks journal February 2015
Characterization of an acetylated heteroxylan from Eucalyptus globulus Labill journal March 2003
(2-O-α-d-Galactopyranosyl-4-O-methyl-α-d-glucurono)-d-xylan from Eucalyptus globulus Labill journal July 1999
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Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term journal April 2005
Features of promising technologies for pretreatment of lignocellulosic biomass journal April 2005
Trends in biotechnological production of fuel ethanol from different feedstocks journal September 2008
Pretreatments to enhance the digestibility of lignocellulosic biomass journal January 2009
Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation☆ journal July 2010
Two-step liquid hot water pretreatment of Eucalyptus grandis to enhance sugar recovery and enzymatic digestibility of cellulose journal July 2010
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Changes in composition and sugar release across the annual rings of Populus wood and implications on recalcitrance journal January 2011
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Effects of hardwood structural and chemical characteristics on enzymatic hydrolysis for biofuel production journal April 2012
Ethanol production from Eucalyptus plantation thinnings journal April 2012
Exploring the effect of different plant lignin content and composition on ionic liquid pretreatment efficiency and enzymatic saccharification of Eucalyptus globulus L. mutants journal August 2012
Demonstration of laccase-based removal of lignin from wood and non-wood plant feedstocks journal September 2012
Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process journal January 2013
Use of new endophytic fungi as pretreatment to enhance enzymatic saccharification of Eucalyptus globulus journal November 2015
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Bioethanol production from autohydrolyzed Eucalyptus globulus by Simultaneous Saccharification and Fermentation operating at high solids loading journal April 2012
Second generation bioethanol from steam exploded Eucalyptus globulus wood journal September 2013
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Regulation of cell wall biosynthesis journal December 2007
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Cellulose synthesis: a complex complex journal June 2008
Overcome the recalcitrance of eucalyptus bark to enzymatic hydrolysis by concerted ionic liquid pretreatment journal December 2015
Biofuel production: Prospects, challenges and feedstock in Australia journal October 2012
Environmental aspects of eucalyptus based ethanol production and use journal November 2012
Biofuel alternatives to ethanol: pumping the microbial well journal July 2008
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Optimization of Simultaneous Saccharification and Fermentation for the Production of Ethanol from Lignocellulosic Biomass journal May 2000
Protic Ionic Liquids:  Solvents with Tunable Phase Behavior and Physicochemical Properties journal November 2006
Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids journal January 2004
Genomics of cellulosic biofuels journal August 2008
Lignin modification improves fermentable sugar yields for biofuel production journal June 2007
Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol journal June 2008
Growth of the plant cell wall journal November 2005
Dissolution of cellulose with ionic liquids and its application: a mini-review journal January 2006
Australian biogeographical connections and the phylogeny of large genera in the plant family Myrtaceae: Australian biogeographical connections journal June 2003
Functional analysis of tobacco LIM protein Ntlim1 involved in lignin biosynthesis journal May 2000
Characterization of cis-elements required for vascular expression of the Cinnamoyl CoA Reductase gene and for protein-DNA complex formation journal September 2000
A Genomic and Molecular View of Wood Formation journal July 2006
Is Eucalyptus Cryptically Self-incompatible? journal September 2007
Lignin Biosynthesis and Structure journal May 2010
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure journal November 2007
Gene expression in Eucalyptus branch wood with marked variation in cellulose microfibril orientation and lacking G-layers journal July 2008
Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth journal January 1993
EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis journal July 2005
Synthesis, regulation and utilization of lignocellulosic biomass journal April 2010
Biosynthesis and incorporation of side-chain-truncated lignin monomers to reduce lignin polymerization and enhance saccharification: Reduction in lignin DP enhances saccharification journal March 2012
Transcript profiling of Eucalyptus xylem genes during tension wood formation journal March 2005
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Association genetics of traits controlling lignin and cellulose biosynthesis in black cottonwood (Populus trichocarpa, Salicaceae) secondary xylem journal September 2010
EgMYB1, an R2R3 MYB transcription factor from eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar journal October 2010
Cellulose crystallinity - a key predictor of the enzymatic hydrolysis rate: Cellulose crystallinity journal February 2010
The fasciclin-like arabinogalactan protein family of Eucalyptus grandis contains members that impact wood biology and biomechanics journal February 2015
Modifying plants for biofuel and biomaterial production journal November 2014
Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production journal February 2007
Feedstocks for Lignocellulosic Biofuels journal August 2010
Estimation of Eucalyptus delegatensis wood properties by near infrared spectroscopy journal January 2001
Comparison of basic density and longitudinal shrinkage in tension wood and opposite wood in young stems of Populus euramericana cv. Ghoy when subjected to a gravitational stimulus journal January 2001
Hemicelluloses journal June 2010
The Genetics of Lignin Biosynthesis: Connecting Genotype to Phenotype journal December 2010
Cellulosic Biofuels journal June 2009
Lignin Biosynthesis journal June 2003
Cellulose Synthesis in Higher Plants journal November 2006
Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges journal November 1999
Combining hot-compressed water and ball milling pretreatments to improve the efficiency of the enzymatic hydrolysis of eucalyptus journal January 2008
A short review on SSF – an interesting process option for ethanol production from lignocellulosic feedstocks journal January 2008
Bioconversion of lignocellulose: inhibitors and detoxification journal January 2013
Effects of pretreatment on morphology, chemical composition and enzymatic digestibility of eucalyptus bark: a potentially valuable source of fermentable sugars for biofuel production – part 1 journal January 2013
Pretreatment with laccase and a phenolic mediator degrades lignin and enhances saccharification of Eucalyptus feedstock journal January 2014
Next-generation biomass feedstocks for biofuel production journal January 2008
Down-regulation of p-coumaroyl quinate/shikimate 3′-hydroxylase (C3′H) and cinnamate 4-hydroxylase (C4H) genes in the lignin biosynthetic pathway of Eucalyptus urophylla × E. grandis leads to improved sugar release journal August 2015
Relationship of Lignin Content, Lignin Monomeric Composition and Hemicellulose Composition in the Same Trunk Sought by their Within-Tree Variations in Eucalyptus camaldulensis and E. globulus journal January 1997
Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis journal December 2006
Energy Product Options for Eucalyptus Species Grown as Short Rotation Woody Crops journal July 2008
Understanding the impact of ionic liquid pretreatment on eucalyptus journal January 2010
Eucalypts as a biofuel feedstock journal November 2011

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