The MGF360-16R ORF of African Swine Fever Virus Strain Georgia Encodes for a Nonessential Gene That Interacts with Host Proteins SERTAD3 and SDCBP

Ramírez-Medina, Elizabeth; Vuono, Elizabeth A.; Velazquez-Salinas, Lauro; Silva, Ediane; Rai, Ayushi; Pruitt, Sarah; Berggren, Keith A.; Zhu, James; Borca, Manuel V.; Gladue, Douglas P.

doi:10.3390/v12010060

Title: The MGF360-16R ORF of African Swine Fever Virus Strain Georgia Encodes for a Nonessential Gene That Interacts with Host Proteins SERTAD3 and SDCBP

Journal Article · 02 January 2020 · Viruses

DOI: https://doi.org/10.3390/v12010060 · OSTI ID:1628591

^[1]; Vuono, Elizabeth A. ^[2]; Velazquez-Salinas, Lauro ^[3]; Silva, Ediane ^[2]; Rai, Ayushi ^[4]; Pruitt, Sarah ^[4];

^[5]; Zhu, James ^[5]; Borca, Manuel V. ^[5];

^[5]

Agricultural Research Service (ARS), Greenport, NY (United States). Plum Island Animal Disease Center; Univ. of Connecticut, Storrs, CT (United States). Dept. of Pathobiology; DOE/OSTI
Agricultural Research Service (ARS), Greenport, NY (United States). Plum Island Animal Disease Center; Mississippi State Univ., Starkville, MS (United States). Dept. of Pathobiology and Population Medicine
Agricultural Research Service (ARS), Greenport, NY (United States). Plum Island Animal Disease Center; Kansas State Univ., Manhattan, KS (United States). College of Veterinary Medicine
Agricultural Research Service (ARS), Greenport, NY (United States). Plum Island Animal Disease Center; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
Agricultural Research Service (ARS), Greenport, NY (United States). Plum Island Animal Disease Center

African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs with significant economic consequences to the swine industry. The ASFV genome encodes for more than 160 genes, but only a few of them have been studied in detail. Here we report the characterization of open reading frame (ORF) MGF360-16R. Kinetic studies of virus RNA transcription demonstrated that the MGF360-16R gene is transcribed as a late virus protein. Analysis of host–protein interactions for the MGF360-16R gene using a yeast two-hybrid screen identified SERTA domain containing 3 (SERTAD3) and syndecan-binding protein (SDCBP) as host protein binding partners. SERTAD3 and SDCBP are both involved in nuclear transcription and SDCBP has been shown to be involved in virus traffic inside the host cell. Interaction between MGF360-16R and SERTAD3 and SDCBP host proteins was confirmed in eukaryotic cells transfected with plasmids expressing MGF360-16R and SERTAD3 or SDCBP fused to fluorescent tags. A recombinant ASFV lacking the MGF360-16R gene (ASFV-G-ΔMGF360-16R) was developed from the highly virulent field isolate Georgia2007 (ASFV-G) and was used to show that MGF360-16R is a nonessential gene. ASFV-G-ΔMGF360-16R had a similar replication ability in primary swine macrophage cell cultures when compared to its parental virus ASFV-G. Experimental infection of domestic pigs showed that ASFV-G-ΔMGF360-16R is as virulent as the parental virus ASFV-G.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1628591

Journal Information:: Viruses, Journal Name: Viruses Journal Issue: 1 Vol. 12; ISSN VIRUBR; ISSN 1999-4915

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge O'Donnell, Vivian; Holinka, Lauren G.; Krug, Peter W. Journal of Virology, Vol. 89, Issue 16 https://doi.org/10.1128/jvi.00969-15	journal	June 2015
Deletion of the African Swine Fever Virus Gene DP148R Does Not Reduce Virus Replication in Culture but Reduces Virus Virulence in Pigs and Induces High Levels of Protection against Challenge Reis, Ana L.; Goatley, Lynnette C.; Jabbar, Tamara Journal of Virology, Vol. 91, Issue 24 https://doi.org/10.1128/jvi.01428-17	journal	October 2017
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African swine fever virus-induced proteins on the plasma membranes of infected cells Alcaraz, C.; Pasamontes, B.; Ruiz Gonzalvo, F. Virology, Vol. 168, Issue 2 https://doi.org/10.1016/0042-6822(89)90283-3	journal	February 1989
Characterization of P30, a highly antigenic membrane and secreted protein of African Swine Fever Virus Afonso, Claudio L.; Alcaraz, Carlos; Brun, Alejandro Virology, Vol. 189, Issue 1 https://doi.org/10.1016/0042-6822(92)90718-5	journal	July 1992
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Sensitivity of African swine fever virus to type I interferon is linked to genes within multigene families 360 and 505 Golding, Josephine P.; Goatley, Lynnette; Goodbourn, Steve Virology, Vol. 493 https://doi.org/10.1016/j.virol.2016.03.019	journal	June 2016
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An African swine fever virus ORF with similarity to C-type lectins is non-essential for growth in swine macrophages in vitro and for virus virulence in domestic swine The EMBL/GenBank accession numbers of sequences reported in this paper are AF017027 (Chiredzi/83/1), AF017028 (Crocodile/96/1), AF017029 (Crocodile/96/3), AF017030 (E70), AF017031 (E75) AF017032 (Haiti 811), AF017033 (Fairfield/96/1), AF017034 (Wildebeeslaagte/96/1), AF017035 (Malawi Lil20/1), AF017036 (Pretoriuskop/96/4), AF017037 (Pretoriuskop/96/5), AF017038 (Tengani) and AF017039 (Uganda ’61). Neilan, J. G.; Borca, M. V.; Lu, Z. Journal of General Virology, Vol. 80, Issue 10 https://doi.org/10.1099/0022-1317-80-10-2693	journal	October 1999
African Swine Fever Virus Georgia Isolate Harboring Deletions of MGF360 and MGF505 Genes Is Attenuated in Swine and Confers Protection against Challenge with Virulent Parental Virus O'Donnell, Vivian; Holinka, Lauren G.; Gladue, Douglas P. Journal of Virology, Vol. 89, Issue 11 https://doi.org/10.1128/JVI.00554-15	journal	March 2015
African Swine Fever Virus Georgia 2007 with a Deletion of Virulence-Associated Gene 9GL (B119L), when Administered at Low Doses, Leads to Virus Attenuation in Swine and Induces an Effective Protection against Homologous Challenge O'Donnell, Vivian; Holinka, Lauren G.; Krug, Peter W. Journal of Virology, Vol. 89, Issue 16 https://doi.org/10.1128/JVI.00969-15	journal	June 2015
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Deletion of the African Swine Fever Virus Gene DP148R Does Not Reduce Virus Replication in Culture but Reduces Virus Virulence in Pigs and Induces High Levels of Protection against Challenge Reis, Ana L.; Goatley, Lynnette C.; Jabbar, Tamara Journal of Virology, Vol. 91, Issue 24 https://doi.org/10.1128/JVI.01428-17	journal	October 2017
The Progressive Adaptation of a Georgian Isolate of African Swine Fever Virus to Vero Cells Leads to a Gradual Attenuation of Virulence in Swine Corresponding to Major Modifications of the Viral Genome Krug, Peter W.; Holinka, Lauren G.; O'Donnell, Vivian Journal of Virology, Vol. 89, Issue 4 https://doi.org/10.1128/JVI.03250-14	journal	December 2014
Multigene families in African swine fever virus: family 360. González, A.; Calvo, V.; Almazán, F. Journal of Virology, Vol. 64, Issue 5 https://doi.org/10.1128/JVI.64.5.2073-2081.1990	journal	January 1990
An IkappaB homolog encoded by African swine fever virus provides a novel mechanism for downregulation of proinflammatory cytokine responses in host macrophages Powell, P. P.; Dixon, L. K.; Parkhouse, R. M. Journal of Virology, Vol. 70, Issue 12 https://doi.org/10.1128/JVI.70.12.8527-8533.1996	journal	December 1996
An African swine fever virus virulence-associated gene NL-S with similarity to the herpes simplex virus ICP34.5 gene. Zsak, L.; Lu, Z.; Kutish, G. F. Journal of virology, Vol. 70, Issue 12 https://doi.org/10.1128/JVI.70.12.8865-8871.1996	journal	January 1996
A Nonessential African Swine Fever Virus Gene UK Is a Significant Virulence Determinant in Domestic Swine Zsak, L.; Caler, E.; Lu, Z. Journal of Virology, Vol. 72, Issue 2 https://doi.org/10.1128/JVI.72.2.1028-1035.1998	journal	February 1998
Deletion of a CD2-Like Gene, 8-DR, from African Swine Fever Virus Affects Viral Infection in Domestic Swine Borca, M. V.; Carrillo, C.; Zsak, L. Journal of Virology, Vol. 72, Issue 4 https://doi.org/10.1128/JVI.72.4.2881-2889.1998	journal	April 1998
An African Swine Fever Virus ERV1-ALRHomologue, 9GL, Affects Virion Maturation and Viral Growth in Macrophages and Viral Virulence in Swine Lewis, T.; Zsak, L.; Burrage, T. G. Journal of Virology, Vol. 74, Issue 3 https://doi.org/10.1128/JVI.74.3.1275-1285.2000	journal	February 2000
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African Swine Fever Virus Multigene Family 360 Genes Affect Virus Replication and Generalization of Infection in Ornithodoros porcinus Ticks Burrage, T. G.; Lu, Z.; Neilan, J. G. Journal of Virology, Vol. 78, Issue 5 https://doi.org/10.1128/JVI.78.5.2445-2453.2004	journal	March 2004
African Swine Fever Virus Structural Protein p54 Is Essential for the Recruitment of Envelope Precursors to Assembly Sites Rodríguez, Javier M.; García-Escudero, Ramón; Salas, María L. Journal of Virology, Vol. 78, Issue 8 https://doi.org/10.1128/JVI.78.8.4299-4313.2004	journal	April 2004
Interaction of CSFV E2 Protein with Swine Host Factors as Detected by Yeast Two-Hybrid System Gladue, Douglas P.; Baker-Bransetter, Ryan; Holinka, Lauren G. PLoS ONE, Vol. 9, Issue 1 https://doi.org/10.1371/journal.pone.0085324	journal	January 2014
Mechanisms of African swine fever virus pathogenesis and immune evasion inferred from gene expression changes in infected swine macrophages Zhu, James J.; Ramanathan, Palaniappan; Bishop, Elizabeth A. PLOS ONE, Vol. 14, Issue 11 https://doi.org/10.1371/journal.pone.0223955	journal	November 2019
Genomic Analysis of Highly Virulent Georgia 2007/1 Isolate of African Swine Fever Virus Chapman, David A. G.; Darby, Alistair C.; Da Silva, Melissa Emerging Infectious Diseases, Vol. 17, Issue 4 https://doi.org/10.3201/eid1704.101283	journal	April 2011
Classical Swine Fever Virus p7 Protein Interacts with Host Protein CAMLG and Regulates Calcium Permeability at the Endoplasmic Reticulum Gladue, Douglas; Largo, Eneko; Holinka, Lauren Viruses, Vol. 10, Issue 9 https://doi.org/10.3390/v10090460	journal	August 2018
A Deep-Sequencing Workflow for the Fast and Efficient Generation of High-Quality African Swine Fever Virus Whole-Genome Sequences Forth, Jan; Forth, Leonie; King, Jacqueline Viruses, Vol. 11, Issue 9 https://doi.org/10.3390/v11090846	journal	September 2019