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Title: Genetic Analysis of the Full-Length gag Gene from the Earliest Korean Subclade B of HIV-1: An Outbreak among Korean Hemophiliacs

Journal Article · · Viruses
DOI: https://doi.org/10.3390/v11060545 · OSTI ID:1628587
ORCiD logo [1];  [1];  [2]
  1. Ulsan National Institute of Science and Technology, Ulsan (Korea, Republic of). College of Medicine. Dept. of Microbiology
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Theoretical Biology and Biophysics Group. HIV Databases
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We determined the earliest full-length HIV-1 gag gene sequences in 110 patients with HIV-1, including 20 hemophiliacs (HPs) and 90 local controls (LCs). The gag gene from stored sera was amplified using RT-PCR, and was subjected to direct sequencing. Phylogenetic analysis indicated that 94 and 16 sequences belonged to the Korean subclade of HIV-1 subtype B (KSB) and subtype B, respectively. A total of 12 signature pattern amino acids were found within the KSB, distinct from the worldwide consensus of subtype B. Within the KSB, the gag gene sequences from donors O and P and those from the 20 HPs comprised two subclusters. In particular, sequences from donor O strongly clustered with those of eight HPs. Moreover, signature pattern analysis indicated that 14 signature nucleotides were shared between the HPs and LCs within KSB (p < 0.01). Among the 14 nucleotides, positions 9 and 5 belonged to clusters O and P, respectively. In conclusion, signature pattern analysis for the gag gene revealed 12 signature pattern residues within the KSB and also confirmed the previous conclusion that the 20 HPs were infected with viruses due to incompletely inactivated clotting factor IX. This study is the first genetic analysis of the HIV-1 gag gene in Korea.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC52-06NA25396
OSTI ID:
1628587
Journal Information:
Viruses, Vol. 11, Issue 6; ISSN 1999-4915
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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Figures / Tables (6)

Table 1(p. 4)table Table 1
Figure 1(p. 5)figure Figure 1
Table 2(p. 6)table Table 2
Table 3(p. 7)table Table 3
Table 4(p. 7)table Table 4
Figure 2(p. 8)figure Figure 2

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
virology
HIV-1 gag gene
Korean subclade of subtype B
phylogenetic analysis
signature pattern
hemophiliacs