An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat
Abstract
A sensitive, rapid, and reproducible imaging surface plasmon resonance (iSPR) biosensor assay was developed to detect T-2 toxin and T-2 toxin-3-glucoside (T2-G) in wheat. In this competitive assay, an amplification strategy was used after conjugating a secondary antibody (Ab2) with gold nanoparticles. Wheat samples were extracted with a methanol/water mixture (80:20 v/v), then diluted with an equal volume of primary antibody (Ab1) for analysis. Matrix-matched calibration curves were prepared to determine T-2 toxin and T2-G. Recovery studies were conducted at three spiking levels in blank wheat. Mean recoveries ranged from 86 to 90%, with relative standard deviations for repeatability (RSDr) of less than 6%. Limits of detection were 1.2 ng/mL of T-2 toxin and 0.9 ng/mL of T2-G, equivalent to their levels in wheat, of 48 and 36 µg/kg, respectively. The developed iSPR assay was rapid and provided enough sensitivity for the monitoring of T-2 toxin/T2-G in wheat. This is the first iSPR assay useful for detecting the “masked” T2-G in wheat.
- Authors:
-
- US Dept. of Agriculture (USDA), Peoria, IL (United States). Mycotoxin Prevention and Applied Microbiology Research Unit, Agricultural Research Service
- Publication Date:
- Research Org.:
- Oak Ridge Associated Univ., Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1628577
- Grant/Contract Number:
- SC0014664; 5010-42000-0049-00D
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Toxins
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 3; Journal ID: ISSN 2072-6651
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Food Science & Technology; Toxicology; biosensor; iSPR; gold nanoparticle; T-2 toxin; T-2 toxin-3-glucoside; wheat
Citation Formats
Hossain, Md, McCormick, Susan, and Maragos, Chris. An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat. United States: N. p., 2018.
Web. doi:10.3390/toxins10030119.
Hossain, Md, McCormick, Susan, & Maragos, Chris. An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat. United States. https://doi.org/10.3390/toxins10030119
Hossain, Md, McCormick, Susan, and Maragos, Chris. Sat .
"An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat". United States. https://doi.org/10.3390/toxins10030119. https://www.osti.gov/servlets/purl/1628577.
@article{osti_1628577,
title = {An Imaging Surface Plasmon Resonance Biosensor Assay for the Detection of T-2 Toxin and Masked T-2 Toxin-3-Glucoside in Wheat},
author = {Hossain, Md and McCormick, Susan and Maragos, Chris},
abstractNote = {A sensitive, rapid, and reproducible imaging surface plasmon resonance (iSPR) biosensor assay was developed to detect T-2 toxin and T-2 toxin-3-glucoside (T2-G) in wheat. In this competitive assay, an amplification strategy was used after conjugating a secondary antibody (Ab2) with gold nanoparticles. Wheat samples were extracted with a methanol/water mixture (80:20 v/v), then diluted with an equal volume of primary antibody (Ab1) for analysis. Matrix-matched calibration curves were prepared to determine T-2 toxin and T2-G. Recovery studies were conducted at three spiking levels in blank wheat. Mean recoveries ranged from 86 to 90%, with relative standard deviations for repeatability (RSDr) of less than 6%. Limits of detection were 1.2 ng/mL of T-2 toxin and 0.9 ng/mL of T2-G, equivalent to their levels in wheat, of 48 and 36 µg/kg, respectively. The developed iSPR assay was rapid and provided enough sensitivity for the monitoring of T-2 toxin/T2-G in wheat. This is the first iSPR assay useful for detecting the “masked” T2-G in wheat.},
doi = {10.3390/toxins10030119},
journal = {Toxins},
number = 3,
volume = 10,
place = {United States},
year = {Sat Mar 10 00:00:00 EST 2018},
month = {Sat Mar 10 00:00:00 EST 2018}
}
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Cited by: 17 works
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Figures / Tables:
Figure 1: T-2 toxin and T2-3-glucoside (T2-G).
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Works referenced in this record:
A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food
journal, April 2010
- Meneely, Julie P.; Sulyok, Michael; Baumgartner, Sabine
- Talanta, Vol. 81, Issue 1-2
Experimental design for in-house validation of a screening immunoassay kit. The case of a multiplex dipstick for Fusarium mycotoxins in cereals
journal, April 2013
- Lattanzio, Veronica M. T.; von Holst, Christoph; Visconti, Angelo
- Analytical and Bioanalytical Chemistry, Vol. 405, Issue 24
Anomericity of T-2 Toxin-glucoside: Masked Mycotoxin in Cereal Crops
journal, January 2015
- McCormick, Susan P.; Kato, Takayuki; Maragos, Chris M.
- Journal of Agricultural and Food Chemistry, Vol. 63, Issue 2
Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS)
journal, December 2011
- Busman, Mark; Poling, Stephen M.; Maragos, Chris M.
- Toxins, Vol. 3, Issue 12
Fusarium langsethiae - a HT-2 and T-2 Toxins Producer that Needs More Attention
journal, October 2012
- Imathiu, Samuel M.; Edwards, Simon G.; Ray, Rumiana V.
- Journal of Phytopathology, Vol. 161, Issue 1
Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR
journal, September 2016
- Karczmarczyk, Aleksandra; Reiner-Rozman, Ciril; Hageneder, Simone
- Analytica Chimica Acta, Vol. 937
Development and Evaluation of Monoclonal Antibodies for the Glucoside of T-2 Toxin (T2-Glc)
journal, July 2013
- Maragos, Chris; Kurtzman, Cletus; Busman, Mark
- Toxins, Vol. 5, Issue 7
Detection of a new Fusarium masked mycotoxin in wheat grain by high-resolution LC–Orbitrap™ MS
journal, October 2011
- Nakagawa, H.; Ohmichi, K.; Sakamoto, S.
- Food Additives & Contaminants: Part A, Vol. 28, Issue 10
Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays
journal, August 1995
- Xiao, H.; Clarke, J. R.; Marquardt, R. R.
- Journal of Agricultural and Food Chemistry, Vol. 43, Issue 8
Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC–MS–MS
journal, July 2009
- Vendl, O.; Berthiller, F.; Crews, C.
- Analytical and Bioanalytical Chemistry, Vol. 395, Issue 5
Masked Mycotoxins: Determination of a Deoxynivalenol Glucoside in Artificially and Naturally Contaminated Wheat by Liquid Chromatography−Tandem Mass Spectrometry
journal, May 2005
- Berthiller, Franz; Dall'Asta, Chiara; Schuhmacher, Rainer
- Journal of Agricultural and Food Chemistry, Vol. 53, Issue 9
Metabolic profile of the masked mycotoxin T-2 toxin-3-glucoside in rats ( in vitro and in vivo ) and humans ( in vitro )
journal, November 2017
- Yang, S.; Van Poucke, C.; Wang, Z.
- World Mycotoxin Journal, Vol. 10, Issue 4
Development of an LC-MS/MS Determination Method for T-2 Toxin and Its Glucoside and Acetyl Derivatives for Estimating the Contamination of Total T-2 Toxins in Staple Flours
journal, May 2018
- Nakagawa, Hiroyuki; Matsuo, Yosuke; McCormick, Susan
- Journal of AOAC INTERNATIONAL, Vol. 101, Issue 3
Signal amplification using colloidal gold in a biolayer interferometry-based immunosensor for the mycotoxin deoxynivalenol
journal, July 2012
- Maragos, C. M.
- Food Additives & Contaminants: Part A, Vol. 29, Issue 7
Study of the natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high-resolution Orbitrap mass spectrometry
journal, June 2015
- Lattanzio, Veronica M. T.; Ciasca, Biancamaria; Terzi, Valeria
- Food Additives & Contaminants: Part A, Vol. 32, Issue 10
Identification and characterization of new Fusarium masked mycotoxins, T2 and HT2 glycosyl derivatives, in naturally contaminated wheat and oats by liquid chromatography-high-resolution mass spectrometry: T2 and HT2 glycosides determination by LC-HRMS
journal, March 2012
- Lattanzio, Veronica M. T.; Visconti, Angelo; Haidukowski, Miriam
- Journal of Mass Spectrometry, Vol. 47, Issue 4
Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags
journal, October 2014
- Hu, Weihua; Chen, Hongming; Zhang, Huanhuan
- Journal of Colloid and Interface Science, Vol. 431
Gold nanoparticle-enhanced multiplexed imaging surface plasmon resonance (iSPR) detection of Fusarium mycotoxins in wheat
journal, March 2018
- Hossain, Md Zakir; Maragos, Chris M.
- Biosensors and Bioelectronics, Vol. 101
Biosensors based on surface plasmon-enhanced fluorescence spectroscopy (Review)
journal, September 2008
- Dostálek, Jakub; Knoll, Wolfgang
- Biointerphases, Vol. 3, Issue 3
Simultaneous determination of deoxynivalenol, zearalenone, and their major masked metabolites in cereal-based food by LC–MS–MS
journal, July 2009
- Vendl, O.; Berthiller, F.; Crews, C.
- Analytical and Bioanalytical Chemistry, Vol. 395, Issue 5
Fast and sensitive detection of ochratoxin A in red wine by nanoparticle-enhanced SPR
journal, September 2016
- Karczmarczyk, Aleksandra; Reiner-Rozman, Ciril; Hageneder, Simone
- Analytica Chimica Acta, Vol. 937
Gold nanoparticle-enhanced multiplexed imaging surface plasmon resonance (iSPR) detection of Fusarium mycotoxins in wheat
journal, March 2018
- Hossain, Md Zakir; Maragos, Chris M.
- Biosensors and Bioelectronics, Vol. 101
Sensitive detection of multiple mycotoxins by SPRi with gold nanoparticles as signal amplification tags
journal, October 2014
- Hu, Weihua; Chen, Hongming; Zhang, Huanhuan
- Journal of Colloid and Interface Science, Vol. 431
A rapid optical immunoassay for the screening of T-2 and HT-2 toxin in cereals and maize-based baby food
journal, April 2010
- Meneely, Julie P.; Sulyok, Michael; Baumgartner, Sabine
- Talanta, Vol. 81, Issue 1-2
Detection of Staphylococcus aureus enterotoxin B at femtomolar levels with a miniature integrated two-channel surface plasmon resonance (SPR) sensor
journal, June 2002
- Naimushin, Alexei N.; Soelberg, Scott D.; Nguyen, Di K.
- Biosensors and Bioelectronics, Vol. 17, Issue 6-7
Improved Methods for Conjugating Selected Mycotoxins to Carrier Proteins and Dextran for Immunoassays
journal, August 1995
- Xiao, H.; Clarke, J. R.; Marquardt, R. R.
- Journal of Agricultural and Food Chemistry, Vol. 43, Issue 8
Masked Mycotoxins: Determination of a Deoxynivalenol Glucoside in Artificially and Naturally Contaminated Wheat by Liquid Chromatography−Tandem Mass Spectrometry
journal, May 2005
- Berthiller, Franz; Dall'Asta, Chiara; Schuhmacher, Rainer
- Journal of Agricultural and Food Chemistry, Vol. 53, Issue 9
Anomericity of T-2 Toxin-glucoside: Masked Mycotoxin in Cereal Crops
journal, January 2015
- McCormick, Susan P.; Kato, Takayuki; Maragos, Chris M.
- Journal of Agricultural and Food Chemistry, Vol. 63, Issue 2
Multiplex surface plasmon resonance biosensing and its transferability towards imaging nanoplasmonics for detection of mycotoxins in barley
journal, January 2016
- Joshi, Sweccha; Segarra-Fas, Anna; Peters, Jeroen
- The Analyst, Vol. 141, Issue 4
Detection of a new Fusarium masked mycotoxin in wheat grain by high-resolution LC–Orbitrap™ MS
journal, October 2011
- Nakagawa, H.; Ohmichi, K.; Sakamoto, S.
- Food Additives & Contaminants: Part A, Vol. 28, Issue 10
Signal amplification using colloidal gold in a biolayer interferometry-based immunosensor for the mycotoxin deoxynivalenol
journal, July 2012
- Maragos, C. M.
- Food Additives & Contaminants: Part A, Vol. 29, Issue 7
Study of the natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high-resolution Orbitrap mass spectrometry
journal, June 2015
- Lattanzio, Veronica M. T.; Ciasca, Biancamaria; Terzi, Valeria
- Food Additives & Contaminants: Part A, Vol. 32, Issue 10
Fusarium langsethiae - a HT-2 and T-2 Toxins Producer that Needs More Attention
journal, October 2012
- Imathiu, Samuel M.; Edwards, Simon G.; Ray, Rumiana V.
- Journal of Phytopathology, Vol. 161, Issue 1
Biosensors based on surface plasmon-enhanced fluorescence spectroscopy (Review)
journal, September 2008
- Dostálek, Jakub; Knoll, Wolfgang
- Biointerphases, Vol. 3, Issue 3
Glucosylation and Other Biotransformations of T-2 Toxin by Yeasts of the Trichomonascus Clade
journal, October 2012
- McCormick, Susan P.; Price, Neil P. J.; Kurtzman, Cletus P.
- Applied and Environmental Microbiology, Vol. 78, Issue 24
Improved method for production of antibodies against T-2 toxin and diacetoxyscirpenol in rabbits.
journal, January 1986
- Zhang, G. S.; Schubring, S. L.; Chu, F. S.
- Applied and Environmental Microbiology, Vol. 51, Issue 1
Observation of T-2 Toxin and HT-2 Toxin Glucosides from Fusarium sporotrichioides by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS)
journal, December 2011
- Busman, Mark; Poling, Stephen M.; Maragos, Chris M.
- Toxins, Vol. 3, Issue 12
Development and Evaluation of Monoclonal Antibodies for the Glucoside of T-2 Toxin (T2-Glc)
journal, July 2013
- Maragos, Chris; Kurtzman, Cletus; Busman, Mark
- Toxins, Vol. 5, Issue 7
Development of an LC-MS/MS Determination Method for T-2 Toxin and Its Glucoside and Acetyl Derivatives for Estimating the Contamination of Total T-2 Toxins in Staple Flours
journal, May 2018
- Nakagawa, Hiroyuki; Matsuo, Yosuke; McCormick, Susan
- Journal of AOAC INTERNATIONAL, Vol. 101, Issue 3
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journal, January 2020
- Garbacz, Monika; Malec, Agnieszka; Duda-Saternus, Sylwia
- Chemosensors, Vol. 8, Issue 1
Recent Advances in Surface Plasmon Resonance Imaging Sensors
journal, March 2019
- Wang, Dongping; Loo, Jacky; Chen, Jiajie
- Sensors, Vol. 19, Issue 6
Recent Advances in Surface Plasmon Resonance Imaging Sensors
journal, March 2019
- Wang, Dongping; Loo, Jacky; Chen, Jiajie
- Sensors, Vol. 19, Issue 6
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