ATP- and Polyphosphate-Dependent Glucokinases from Aerobic Methanotrophs

Journal Article · 14 February 2019 · Microorganisms

DOI: https://doi.org/10.3390/microorganisms7020052 · OSTI ID:1628467

Reshetnikov, Alexander ^[1]; Solntseva, Natalia ^[2]; Rozova, Olga ^[3]; Mustakhimov, Ildar ^[4]; Trotsenko, Yuri ^[4]; Khmelenina, Valentina ^[5]

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1. Russian Academy of Sciences (RAS), Pushchino (Russian Federation). Federal Research Center. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences. G.K. Skryabin Inst. of Biochemistry and Physiology of Microorganisms. Lab. of Methylotrophy; DOE/OSTI
2. Pushchino State Inst. of Natural Sciences, Pushchino (Russian Federation). Dept. of Microbiology and Biotechnology
3. Russian Academy of Sciences (RAS), Pushchino (Russian Federation). Federal Research Center. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences. G.K. Skryabin Inst. of Biochemistry and Physiology of Microorganisms. Lab. of Methylotrophy
4. Russian Academy of Sciences (RAS), Pushchino (Russian Federation). Federal Research Center. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences. G.K. Skryabin Inst. of Biochemistry and Physiology of Microorganisms; Pushchino State Inst. of Natural Sciences, Pushchino (Russian Federation). Dept. of Microbiology and Biotechnology
5. Russian Academy of Sciences (RAS), Pushchino (Russian Federation). Federal Research Center. Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences. G.K. Skryabin Inst. of Biochemistry and Physiology of Microorganisms

The genes encoding adenosine triphosphate (ATP)- and polyphosphate (polyP)-dependent glucokinases (Glk) were identified in the aerobic obligate methanotroph Methylomonas sp. 12. The recombinant proteins were obtained by the heterologous expression of the glk genes in Esherichia coli. ATP-Glk behaved as a multimeric protein consisting of di-, tri-, tetra-, penta- and hexamers with a subunit molecular mass of 35.5 kDa. ATP-Glk phosphorylated glucose and glucosamine using ATP (100% activity), uridine triphosphate (UTP) (85%) or guanosine triphosphate (GTP) (71%) as a phosphoryl donor and exhibited the highest activity in the presence of 5 mM Mg²⁺ at pH 7.5 and 65 °C but was fully inactivated after a short-term incubation at this temperature. According to a gel filtration in the presence of polyP, the polyP-dependent Glk was a dimeric protein (2 × 28 kDa). PolyP-Glk phosphorylated glucose, mannose, 2-deoxy-D-glucose, glucosamine and N-acetylglucosamine using polyP as the phosphoryl donor but not using nucleoside triphosphates. The K_m values of ATP-Glk for glucose and ATP were about 78 μM, and the K_m values of polyP-Glk for glucose and polyP_(n=45) were 450 and 21 μM, respectively. The genomic analysis of methanotrophs showed that ATP-dependent glucokinase is present in all sequenced methanotrophs, with the exception of the genera Methylosinus and Methylocystis, whereas polyP-Glks were found in all species of the genus Methylomonas and in Methylomarinum vadi only. This work presents the first characterization of polyphosphate specific glucokinase in a methanotrophic bacterium.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division
• Grant/Contract Number: AC02-05CH11231
• OSTI ID: 1628467
• Journal Information: Microorganisms, Journal Name: Microorganisms Journal Issue: 2 Vol. 7; ISSN 2076-2607
• Publisher: MDPICopyright Statement
• Country of Publication: United States
• Language: English