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Title: The Fungal Cell Death Regulator czt-1 Is Allelic to acr-3

Journal Article · · Journal of Fungi
DOI: https://doi.org/10.3390/jof5040114 · OSTI ID:1628395
ORCiD logo [1];  [2];  [3]; ORCiD logo [1]
  1. University of Porto (Portugal)
  2. Kansas State University, Manhattan, KS (United States)
  3. University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Fungal infections have far-reaching implications that range from severe human disease to a panoply of disruptive agricultural and ecological effects, making it imperative to identify and understand the molecular pathways governing the response to antifungal compounds. In this context, CZT-1 (cell death-activated zinc cluster transcription factor) functions as a master regulator of cell death and drug susceptibility in Neurospora crassa. Here we provide evidence indicating that czt-1 is allelic to acr-3, a previously described locus that we now found to harbor a point mutation in its coding sequence. This nonsynonymous amino acid substitution in a low complexity region of CZT-1/ACR-3 caused a robust gain-of-function that led to reduced sensitivity to acriflavine and staurosporine, and increased expression of the drug efflux pump abc-3. Thus, accumulating evidence shows that CZT-1 is an important broad regulator of the cellular response to various antifungal compounds that appear to share common molecular targets.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE; FCT Portugal; FEDER; National Science Foundation (NSF)
Grant/Contract Number:
AC02-05CH11231; PEst-C/SAU/LA0002/2013; FCOMP-01-0124-FEDER-037277; NORTE-07-0124-FEDER-000003; DBI 0742713
OSTI ID:
1628395
Journal Information:
Journal of Fungi, Vol. 5, Issue 4; ISSN 2309-608X
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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