The Fungal Cell Death Regulator czt-1 Is Allelic to acr-3
- University of Porto (Portugal)
- Kansas State University, Manhattan, KS (United States)
- University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Fungal infections have far-reaching implications that range from severe human disease to a panoply of disruptive agricultural and ecological effects, making it imperative to identify and understand the molecular pathways governing the response to antifungal compounds. In this context, CZT-1 (cell death-activated zinc cluster transcription factor) functions as a master regulator of cell death and drug susceptibility in Neurospora crassa. Here we provide evidence indicating that czt-1 is allelic to acr-3, a previously described locus that we now found to harbor a point mutation in its coding sequence. This nonsynonymous amino acid substitution in a low complexity region of CZT-1/ACR-3 caused a robust gain-of-function that led to reduced sensitivity to acriflavine and staurosporine, and increased expression of the drug efflux pump abc-3. Thus, accumulating evidence shows that CZT-1 is an important broad regulator of the cellular response to various antifungal compounds that appear to share common molecular targets.
- Research Organization:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Organization:
- USDOE; FCT Portugal; FEDER; National Science Foundation (NSF)
- Grant/Contract Number:
- AC02-05CH11231; PEst-C/SAU/LA0002/2013; FCOMP-01-0124-FEDER-037277; NORTE-07-0124-FEDER-000003; DBI 0742713
- OSTI ID:
- 1628395
- Journal Information:
- Journal of Fungi, Vol. 5, Issue 4; ISSN 2309-608X
- Publisher:
- MDPICopyright Statement
- Country of Publication:
- United States
- Language:
- English
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