Plant Hormones Differentially Control the Sub-Cellular Localization of Plasma Membrane Microdomains during the Early Stage of Soybean Nodulation

Qiao, Zhenzhen; Zogli, Prince; Libault, Marc

doi:10.3390/genes10121012

Title: Plant Hormones Differentially Control the Sub-Cellular Localization of Plasma Membrane Microdomains during the Early Stage of Soybean Nodulation

Journal Article · 04 December 2019 · Genes

DOI: https://doi.org/10.3390/genes10121012 · OSTI ID:1628321

Qiao, Zhenzhen ^[1]; Zogli, Prince ^[2]; Libault, Marc ^[2]

Univ. of Oklahoma, Norman, OK (United States). Dept. of Microbiology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division; DOE/OSTI
Univ. of Nebraska, Lincoln, NE (United States). Dept. of Agronomy and Horticulture

Phytohormones regulate the mutualistic symbiotic interaction between legumes and rhizobia, nitrogen-fixing soil bacteria, notably by controlling the formation of the infection thread in the root hair (RH). At the cellular level, the formation of the infection thread is promoted by the translocation of plasma membrane microdomains at the tip of the RH. We hypothesize that phytohormones regulate the translocation of plasma membrane microdomains to regulate infection thread formation. Accordingly, we treated with hormone and hormone inhibitors transgenic soybean roots expressing fusions between the Green Fluorescent Protein (GFP) and GmFWL1 or GmFLOT2/4, two microdomain-associated proteins translocated at the tip of the soybean RH in response to rhizobia. Auxin and cytokinin treatments are sufficient to trigger or inhibit the translocation of GmFWL1 and GmFLOT2/4 to the RH tip independently of the presence of rhizobia, respectively. Unexpectedly, the application of salicylic acid, a phytohormone regulating the plant defense system, also promotes the translocation of GmFWL1 and GmFLOT2/4 to the RH tip regardless of the presence of rhizobia. These results suggest that phytohormones are playing a central role in controlling the early stages of rhizobia infection by regulating the translocation of plasma membrane microdomains. They also support the concept of crosstalk of phytohormones to control nodulation.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1628321

Journal Information:: Genes, Journal Name: Genes Journal Issue: 12 Vol. 10; ISSN 2073-4425

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Symbiotic root infections in Medicago truncatula require remorin-mediated receptor stabilization in membrane nanodomains Liang, Pengbo; Stratil, Thomas F.; Popp, Claudia Proceedings of the National Academy of Sciences, Vol. 115, Issue 20 https://doi.org/10.1073/pnas.1721868115	journal	April 2018
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Root hair curling and Rhizobium infection in Medicago truncatula are mediated by phosphatidylinositide-regulated endocytosis and reactive oxygen species Peleg-Grossman, S.; Volpin, H.; Levine, A. Journal of Experimental Botany, Vol. 58, Issue 7 https://doi.org/10.1093/jxb/erm013	journal	April 2007
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GmYUC2a mediates auxin biosynthesis during root development and nodulation in soybean Wang, Youning; Yang, Wei; Zuo, Yanyan Journal of Experimental Botany, Vol. 70, Issue 12 https://doi.org/10.1093/jxb/erz144	journal	April 2019
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Salicylic Acid Inhibits Indeterminate-Type Nodulation But Not Determinate-Type Nodulation van Spronsen, Paulina C.; Tak, Teun; Rood, Anita M. M. Molecular Plant-Microbe Interactions®, Vol. 16, Issue 1 https://doi.org/10.1094/MPMI.2003.16.1.83	journal	January 2003
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