Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition
Abstract
GAlactUronosylTransferase12 (GAUT12)/IRregular Xylem8 (IRX8) is a putative glycosyltransferase involved in Arabidopsis secondary cell wall biosynthesis. Previous work showed that Arabidopsis irregular xylem8 (irx8) mutants have collapsed xylem due to a reduction in xylan and a lesser reduction in a subfraction of homogalacturonan (HG). We now show that male sterility in the irx8 mutant is due to indehiscent anthers caused by reduced deposition of xylan and lignin in the endothecium cell layer. The reduced lignin content was demonstrated by histochemical lignin staining and pyrolysis Molecular Beam Mass Spectrometry (pyMBMS) and is associated with reduced lignin biosynthesis in irx8 stems. Examination of sequential chemical extracts of stem walls using 2D 13C-1H Heteronuclear Single-Quantum Correlation (HSQC) NMR spectroscopy and antibody-based glycome profiling revealed a reduction in G lignin in the 1 M KOH extract and a concomitant loss of xylan, arabinogalactan and pectin epitopes in the ammonium oxalate, sodium carbonate, and 1 M KOH extracts from the irx8 walls compared with wild-type walls. Immunolabeling of stem sections using the monoclonal antibody CCRC-M138 reactive against an unsubstituted xylopentaose epitope revealed a bi-lamellate pattern in wild-type fiber cells and a collapsed bi-layer in irx8 cells, suggesting that at least in fiber cells, GAUT12 participates inmore »
- Authors:
-
- Univ. of Georgia, Athens, GA (United States). Dept. of Plant Biology; Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Univ. of Georgia, Athens, GA (United States). Dept. of Chemistry
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Lawrence Univ., Appleton, WI (United States). Dept. of Biology
- Univ. of Georgia, Athens, GA (United States). Dept. of Plant Biology
- Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Georgia, Athens, GA (United States). Dept. of Biochemistry and Molecular Biology
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division
- OSTI Identifier:
- 1628260
- Grant/Contract Number:
- FG02-93ER20097; FG05-93ER20097; AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 5; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; secondary cell walls; xylan; lignin; pectin; wall glycan epitopes; anther dehiscence
Citation Formats
Hao, Zhangying, Avci, Utku, Tan, Li, Zhu, Xiang, Glushka, John, Pattathil, Sivakumar, Eberhard, Stefan, Sholes, Tipton, Rothstein, Grace E., Lukowitz, Wolfgang, Orlando, Ron, Hahn, Michael G., and Mohnen, Debra. Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition. United States: N. p., 2014.
Web. doi:10.3389/fpls.2014.00357.
Hao, Zhangying, Avci, Utku, Tan, Li, Zhu, Xiang, Glushka, John, Pattathil, Sivakumar, Eberhard, Stefan, Sholes, Tipton, Rothstein, Grace E., Lukowitz, Wolfgang, Orlando, Ron, Hahn, Michael G., & Mohnen, Debra. Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition. United States. https://doi.org/10.3389/fpls.2014.00357
Hao, Zhangying, Avci, Utku, Tan, Li, Zhu, Xiang, Glushka, John, Pattathil, Sivakumar, Eberhard, Stefan, Sholes, Tipton, Rothstein, Grace E., Lukowitz, Wolfgang, Orlando, Ron, Hahn, Michael G., and Mohnen, Debra. Mon .
"Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition". United States. https://doi.org/10.3389/fpls.2014.00357. https://www.osti.gov/servlets/purl/1628260.
@article{osti_1628260,
title = {Loss of Arabidopsis GAUT12/IRX8 causes anther indehiscence and leads to reduced G lignin associated with altered matrix polysaccharide deposition},
author = {Hao, Zhangying and Avci, Utku and Tan, Li and Zhu, Xiang and Glushka, John and Pattathil, Sivakumar and Eberhard, Stefan and Sholes, Tipton and Rothstein, Grace E. and Lukowitz, Wolfgang and Orlando, Ron and Hahn, Michael G. and Mohnen, Debra},
abstractNote = {GAlactUronosylTransferase12 (GAUT12)/IRregular Xylem8 (IRX8) is a putative glycosyltransferase involved in Arabidopsis secondary cell wall biosynthesis. Previous work showed that Arabidopsis irregular xylem8 (irx8) mutants have collapsed xylem due to a reduction in xylan and a lesser reduction in a subfraction of homogalacturonan (HG). We now show that male sterility in the irx8 mutant is due to indehiscent anthers caused by reduced deposition of xylan and lignin in the endothecium cell layer. The reduced lignin content was demonstrated by histochemical lignin staining and pyrolysis Molecular Beam Mass Spectrometry (pyMBMS) and is associated with reduced lignin biosynthesis in irx8 stems. Examination of sequential chemical extracts of stem walls using 2D 13C-1H Heteronuclear Single-Quantum Correlation (HSQC) NMR spectroscopy and antibody-based glycome profiling revealed a reduction in G lignin in the 1 M KOH extract and a concomitant loss of xylan, arabinogalactan and pectin epitopes in the ammonium oxalate, sodium carbonate, and 1 M KOH extracts from the irx8 walls compared with wild-type walls. Immunolabeling of stem sections using the monoclonal antibody CCRC-M138 reactive against an unsubstituted xylopentaose epitope revealed a bi-lamellate pattern in wild-type fiber cells and a collapsed bi-layer in irx8 cells, suggesting that at least in fiber cells, GAUT12 participates in the synthesis of a specific layer or type of xylan or helps to provide an architecture framework required for the native xylan deposition pattern. The results support the hypothesis that GAUT12 functions in the synthesis of a structure required for xylan and lignin deposition during secondary cell wall formation.},
doi = {10.3389/fpls.2014.00357},
journal = {Frontiers in Plant Science},
number = ,
volume = 5,
place = {United States},
year = {Mon Jul 28 00:00:00 EDT 2014},
month = {Mon Jul 28 00:00:00 EDT 2014}
}
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Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyltransferase
journal, March 2006
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The final split: the regulation of anther dehiscence
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