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Title: Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [3];  [4];  [4];  [3];  [4];  [5];  [5];  [6];  [3]
  1. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology
  2. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology; Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  3. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology
  4. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France); Beijing Forestry University, Beijing (China). Beijing Advanced Innovation Center for Tree Breeding by Molecular Design

The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrhizal roots of Vaccinium myrtillus colonized by a metal tolerant isolate of the fungus Oidiodendron maius as compared to non-mycorrhizal roots. To better understand this phenotype, we applied Next Generation Sequencing technologies to analyze gene expression in V. myrtillus and O. maius Zn grown under normal and Cd-stressed conditions, in the free living and in the mycorrhizal status. The results clearly showed that Cd had a stronger impact on plant gene expression than symbiosis, whereas fungal gene expression was mainly regulated by symbiosis. The higher abundance of transcripts coding for stress related proteins in non-mycorrhizal roots may be related to the higher Cd content. Regulated plant metal transporters have been identified that may play a role in reducing Cd content in mycorrhizal roots exposed to this metal.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1628186
Journal Information:
Frontiers in Microbiology, Vol. 11; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

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journal April 2010
The Structure and Function of the Ericoid Mycorrhizal Root journal April 1996
The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s) journal November 2010
Model systems to unravel the molecular mechanisms of heavy metal tolerance in the ericoid mycorrhizal symbiosis journal December 2015
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How metal-tolerant ecotypes of ectomycorrhizal fungi protect plants from heavy metal pollution journal January 2011
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Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress journal June 2005
Evolution and function of phytochelatin synthases journal February 2006
DNA repair systems as targets of cadmium toxicity journal June 2006
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Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris journal July 1981
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Common and metal-specific proteomic responses to cadmium and zinc in the metal tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn journal January 2015
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Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast journal April 2008
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Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa) journal March 2011
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice journal June 2011
Genome-wide identification of Brassica napus microRNAs and their targets in response to cadmium journal July 2012
Genome-wide identification and characterization of cadmium-responsive microRNAs and their target genes in radish (Raphanus sativus L.) roots journal September 2013
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Defective Copper Transport in the copt5 Mutant Affects Cadmium Tolerance journal November 2014
Laccases Direct Lignification in the Discrete Secondary Cell Wall Domains of Protoxylem journal August 2014
Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance journal July 2006
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs journal July 2013
Secretion of small proteins is species-specific within Aspergillus sp journal May 2016
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal P isolithus albus isolated from ultramafic soil: Metal efflux in Ni-tolerant Pisolithus albus journal June 2014
Tolerance and co-tolerance of the grass Chloris barbata Sw. to mercury, cadmium and zinc journal September 1994
Copper homeostasis: Tansley review journal April 2009
Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants journal November 2018
miR408 is involved in abiotic stress responses in Arabidopsis journal October 2015
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction journal June 2004
The American cranberry: first insights into the whole genome of a species adapted to bog habitat journal January 2014
Identification and Comparative Analysis of Cadmium Tolerance-Associated miRNAs and Their Targets in Two Soybean Genotypes journal December 2013
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants journal December 2014
MicroRNAs as regulators in plant metal toxicity response journal January 2012