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Title: Modulation of Plant and Fungal Gene Expression Upon Cd Exposure and Symbiosis in Ericoid Mycorrhizal Vaccinium myrtillus

Journal Article · · Frontiers in Microbiology
 [1];  [2];  [3];  [4];  [4];  [3];  [4];  [5];  [5];  [6];  [3]
  1. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology
  2. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology; Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  3. Univ. of Turin (Italy). Dept. of Life Sciences and Systems Biology
  4. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France)
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Centre INRAE Grand Est Nancy, Champenoux (France); Beijing Forestry University, Beijing (China). Beijing Advanced Innovation Center for Tree Breeding by Molecular Design
+ Show Author Affiliations

The success of Ericaceae in stressful habitats enriched in heavy metals has been ascribed to the distinctive abilities of their mycorrhizal fungal partners to withstand heavy metal stress and to enhance metal tolerance in the host plant. Whereas heavy metal tolerance has been extensively investigated in some ericoid mycorrhizal (ERM) fungi, the molecular and cellular mechanisms that extend tolerance to the host plant are currently unknown. Here, we show a reduced Cd content in Cd-exposed mycorrhizal roots of Vaccinium myrtillus colonized by a metal tolerant isolate of the fungus Oidiodendron maius as compared to non-mycorrhizal roots. To better understand this phenotype, we applied Next Generation Sequencing technologies to analyze gene expression in V. myrtillus and O. maius Zn grown under normal and Cd-stressed conditions, in the free living and in the mycorrhizal status. The results clearly showed that Cd had a stronger impact on plant gene expression than symbiosis, whereas fungal gene expression was mainly regulated by symbiosis. The higher abundance of transcripts coding for stress related proteins in non-mycorrhizal roots may be related to the higher Cd content. Regulated plant metal transporters have been identified that may play a role in reducing Cd content in mycorrhizal roots exposed to this metal.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1628186
Journal Information:
Frontiers in Microbiology, Vol. 11; ISSN 1664-302X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English

References (125)

Identification and Comparative Analysis of Cadmium Tolerance-Associated miRNAs and Their Targets in Two Soybean Genotypes journal December 2013
Response to cadmium in higher plants journal April 1999
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice journal June 2011
Origin, Biogenesis, and Activity of Plant MicroRNAs journal February 2009
Cadmium: cellular effects, modifications of biomolecules, modulation of DNA repair and genotoxic consequences (a review) journal November 2006
Plant Pleiotropic Drug Resistance Transporters: Transport Mechanism, Gene Expression, and Function: Plant PDR: Mechanism, expression, and function journal May 2014
Insights into the development of fungal biomarkers for metal ecotoxicity assessment: Case of Trametes versicolor exposed to copper
  • Lebrun, Jérémie D.; Trinsoutrot-Gattin, Isabelle; Laval, Karine
  • Environmental Toxicology and Chemistry, Vol. 29, Issue 4 https://doi.org/10.1002/etc.101
journal April 2010
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs journal July 2013
Genome-wide analysis of thiourea-modulated salinity stress-responsive transcripts in seeds of Brassica juncea: identification of signalling and effector components of stress tolerance journal August 2010
Model systems to unravel the molecular mechanisms of heavy metal tolerance in the ericoid mycorrhizal symbiosis journal December 2015
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal P isolithus albus isolated from ultramafic soil: Metal efflux in Ni-tolerant Pisolithus albus journal June 2014
Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants journal November 2018
Structural Biology of the Major Facilitator Superfamily Transporters journal June 2015
Phytochelatins and Their Roles in Heavy Metal Detoxification journal July 2000
Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris journal July 1981
Nramp5 Is a Major Transporter Responsible for Manganese and Cadmium Uptake in Rice journal May 2012
Genome-wide identification and characterization of cadmium-responsive microRNAs and their target genes in radish (Raphanus sativus L.) roots journal September 2013
Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress journal June 2005
Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants journal February 2012
Inventory and general analysis of the ATP-binding cassette (ABC) gene superfamily in maize (Zea mays L.) journal September 2013
How metal-tolerant ecotypes of ectomycorrhizal fungi protect plants from heavy metal pollution journal January 2011
Common and metal-specific proteomic responses to cadmium and zinc in the metal tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn journal January 2015
The SlZRT1 Gene Encodes a Plasma Membrane-Located ZIP (Zrt-, Irt-Like Protein) Transporter in the Ectomycorrhizal Fungus Suillus luteus journal November 2017
A Secreted Effector Protein of Laccaria bicolor Is Required for Symbiosis Development journal July 2011
The NRAMP family of metal-ion transporters journal July 2006
Secretion of small proteins is species-specific within Aspergillus sp journal May 2016
Yeast expression of mammalian Onzin and fungal FCR1 suggests ancestral functions of PLAC8 proteins in mitochondrial metabolism and DNA repair journal April 2019
Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast journal April 2008
The role of ectomycorrhizas in heavy metal stress tolerance of host plants journal December 2014
The Biology of Mycorrhiza in the Ericaceae. Viii. the role of Mycorrhizal Infection in Heavy Metal Resistance journal June 1982
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction journal June 2004
Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete Hebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis journal July 2015
Full-length transcriptome assembly from RNA-Seq data without a reference genome journal May 2011
The copper microRNAs journal October 2016
Defective Copper Transport in the copt5 Mutant Affects Cadmium Tolerance journal November 2014
Genome-wide identification of Cd-responsive NRAMP transporter genes and analyzing expression of NRAMP 1 mediated by miR167 in Brassica napus journal October 2017
Arbuscular mycorrhizal fungus modulates the phytotoxicity of Cd via combined responses of enzymes, thiolic compounds, and essential elements in the roots of Phragmites australis journal November 2017
The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s) journal November 2010
The Oligopeptide Transporters: A Small Gene Family with a Diverse Group of Substrates and Functions? journal May 2011
Laccases Direct Lignification in the Discrete Secondary Cell Wall Domains of Protoxylem journal August 2014
The heavy metal paradox in arbuscular mycorrhizas: from mechanisms to biotechnological applications journal October 2016
Parlez-vous effectors?: Editorial journal August 2010
Phenylpropanoid Biosynthesis journal January 2010
Tolerance and co-tolerance of the grass Chloris barbata Sw. to mercury, cadmium and zinc journal September 1994
The Transporter Classification Database (TCDB): recent advances journal November 2015
Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants journal November 2006
P HYTOCHELATINS AND M ETALLOTHIONEINS : Roles in Heavy Metal Detoxification and Homeostasis journal June 2002
Influence of organic matter on the uptake of cadmium, zinc, copper and iron by sorghum plants journal June 2004
The Structure and Function of the Ericoid Mycorrhizal Root journal April 1996
High-Affinity Manganese Uptake by the Metal Transporter NRAMP1 Is Essential for Arabidopsis Growth in Low Manganese Conditions journal March 2010
Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists journal February 2015
MicroRNAs as regulators in plant metal toxicity response journal January 2012
Ericoid mycorrhiza: a partnership that exploits harsh edaphic conditions journal December 2003
A role for small secreted proteins (SSPs) in a saprophytic fungal lifestyle: Ligninolytic enzyme regulation in Pleurotus ostreatus journal November 2017
Cupredoxins—A study of how proteins may evolve to use metals for bioenergetic processes journal January 2011
The NRAMP6 metal transporter contributes to cadmium toxicity journal August 2009
miR408 is involved in abiotic stress responses in Arabidopsis journal October 2015
microRNA-directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis: Post-transcriptional control of superoxide dismutases journal February 2010
Cadmium toxicity in crop plants and its alleviation by arbuscular mycorrhizal (AM) fungi: An overview journal April 2013
Evolution and function of phytochelatin synthases journal February 2006
Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance journal July 2006
The CTR/COPT-dependent copper uptake and SPL7-dependent copper deficiency responses are required for basal cadmium tolerance in A. thaliana journal January 2013
Mechanisms of metal toxicity in plants journal January 2016
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants journal December 2014
MicroRNA mediated regulation of metal toxicity in plants: present status and future perspectives journal August 2013
The American cranberry: first insights into the whole genome of a species adapted to bog habitat journal January 2014
Genome-wide identification of Brassica napus microRNAs and their targets in response to cadmium journal July 2012
Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa) journal March 2011
DNA repair systems as targets of cadmium toxicity journal June 2006
Analysis and characterization of cultivable extremophilic hydrolytic bacterial community in heavy-metal-contaminated soils from the Atacama Desert and their biotechnological potentials journal July 2012
Genome-wide identification of rice class I metallothionein gene: tissue expression patterns and induction in response to heavy metal stress journal October 2012
Copper homeostasis: Tansley review journal April 2009
The biochemistry of environmental heavy metal uptake by plants: Implications for the food chain journal August 2009
Insights into the development of fungal biomarkers for metal ecotoxicity assessment: Case of Trametes versicolor exposed to copper
  • Lebrun, Jérémie D.; Trinsoutrot-Gattin, Isabelle; Laval, Karine
  • Environmental Toxicology and Chemistry, Vol. 29, Issue 4 https://doi.org/10.1002/etc.101
journal April 2010
The Structure and Function of the Ericoid Mycorrhizal Root journal April 1996
The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s) journal November 2010
Model systems to unravel the molecular mechanisms of heavy metal tolerance in the ericoid mycorrhizal symbiosis journal December 2015
Genome-wide identification of rice class I metallothionein gene: tissue expression patterns and induction in response to heavy metal stress journal October 2012
Genome-wide identification of Cd-responsive NRAMP transporter genes and analyzing expression of NRAMP 1 mediated by miR167 in Brassica napus journal October 2017
MicroRNA mediated regulation of metal toxicity in plants: present status and future perspectives journal August 2013
How metal-tolerant ecotypes of ectomycorrhizal fungi protect plants from heavy metal pollution journal January 2011
Role of miRNAs and siRNAs in biotic and abiotic stress responses of plants journal February 2012
The NRAMP family of metal-ion transporters journal July 2006
The biochemistry of environmental heavy metal uptake by plants: Implications for the food chain journal August 2009
Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants journal November 2006
Origin, Biogenesis, and Activity of Plant MicroRNAs journal February 2009
Arbuscular mycorrhizal fungus modulates the phytotoxicity of Cd via combined responses of enzymes, thiolic compounds, and essential elements in the roots of Phragmites australis journal November 2017
A Secreted Effector Protein of Laccaria bicolor Is Required for Symbiosis Development journal July 2011
The role of ectomycorrhizas in heavy metal stress tolerance of host plants journal December 2014
Differential gene expressions in arbuscular mycorrhizal-colonized tomato grown under heavy metal stress journal June 2005
Evolution and function of phytochelatin synthases journal February 2006
DNA repair systems as targets of cadmium toxicity journal June 2006
Response to cadmium in higher plants journal April 1999
Mycorrhizal infection and resistance to heavy metal toxicity in Calluna vulgaris journal July 1981
Full-length transcriptome assembly from RNA-Seq data without a reference genome journal May 2011
Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists journal February 2015
A role for small secreted proteins (SSPs) in a saprophytic fungal lifestyle: Ligninolytic enzyme regulation in Pleurotus ostreatus journal November 2017
Cupredoxins—A study of how proteins may evolve to use metals for bioenergetic processes journal January 2011
Common and metal-specific proteomic responses to cadmium and zinc in the metal tolerant ericoid mycorrhizal fungus Oidiodendron maius Zn journal January 2015
The NRAMP6 metal transporter contributes to cadmium toxicity journal August 2009
Ericoid mycorrhiza: a partnership that exploits harsh edaphic conditions journal December 2003
Expression of the Novel Wheat Gene TM20 Confers Enhanced Cadmium Tolerance to Bakers' Yeast journal April 2008
Cadmium toxicity in crop plants and its alleviation by arbuscular mycorrhizal (AM) fungi: An overview journal April 2013
Genome-wide analysis of thiourea-modulated salinity stress-responsive transcripts in seeds of Brassica juncea: identification of signalling and effector components of stress tolerance journal August 2010
Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa) journal March 2011
The OsNRAMP1 iron transporter is involved in Cd accumulation in rice journal June 2011
Genome-wide identification of Brassica napus microRNAs and their targets in response to cadmium journal July 2012
Genome-wide identification and characterization of cadmium-responsive microRNAs and their target genes in radish (Raphanus sativus L.) roots journal September 2013
The heavy metal paradox in arbuscular mycorrhizas: from mechanisms to biotechnological applications journal October 2016
The Oligopeptide Transporters: A Small Gene Family with a Diverse Group of Substrates and Functions? journal May 2011
Defective Copper Transport in the copt5 Mutant Affects Cadmium Tolerance journal November 2014
Laccases Direct Lignification in the Discrete Secondary Cell Wall Domains of Protoxylem journal August 2014
Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis Is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance journal July 2006
Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs journal July 2013
Secretion of small proteins is species-specific within Aspergillus sp journal May 2016
Evidence of nickel (Ni) efflux in Ni-tolerant ectomycorhizal P isolithus albus isolated from ultramafic soil: Metal efflux in Ni-tolerant Pisolithus albus journal June 2014
Tolerance and co-tolerance of the grass Chloris barbata Sw. to mercury, cadmium and zinc journal September 1994
Copper homeostasis: Tansley review journal April 2009
Physiological and molecular mechanisms of heavy metal accumulation in nonmycorrhizal versus mycorrhizal plants journal November 2018
miR408 is involved in abiotic stress responses in Arabidopsis journal October 2015
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction journal June 2004
The American cranberry: first insights into the whole genome of a species adapted to bog habitat journal January 2014
Identification and Comparative Analysis of Cadmium Tolerance-Associated miRNAs and Their Targets in Two Soybean Genotypes journal December 2013
Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants journal December 2014
MicroRNAs as regulators in plant metal toxicity response journal January 2012

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Related Subjects

Microbiology
Cd tolerance
transcriptomics
ericoid mycorrhiza
Oidiodendron maius
Vaccinium myrtillus