In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)
Abstract
Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionizationmass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionization-multiple stage mass spectrometry. Our results show multiple lines of evidence for the production of bGDGTs in sediments and cellulolytic enrichments in a hot spring (62–86°C) in the Great Basin (USA). First, in situ cellulolytic enrichment led to an increase in the relative abundance of hydrolysis-derived P-bGDGTs over their C-bGDGT counterparts. Second, the hydrolysisderived P- and C-bGDGT profiles in the hot spring were different from those of the surrounding soil samples; in particular, a monoglycosidic bGDGT Ib containing 13,16- dimethyloctacosane and one cyclopentane moiety was detected in the TLE but it was undetectable in surrounding soil samples even after sample enrichments. Third, previously published 16S rRNA gene pyrotag analysis from the same lignocellulose samples demonstrated the enrichment of thermophiles, rather than mesophiles, and total bGDGT abundance in cellulolytic enrichments correlated with the relative abundance of 16S rRNA gene pyrotags from thermophilic bacteria in the phyla Bacteroidetes, Dictyoglomi, EM3, and OP9 (“Atribacteria”). These observationsmore »
- Authors:
-
- Univ. of Georgia, Athens, GA (United States). Dept. of Marine Sciences; Tongji Univ., Shanghai (China). State Key Lab. of Marine Geology
- Univ. of Nevada, Las Vegas, NV (United States). School of Life Sciences
- Univ. of Bremen (Germany). MARUM Center for Marine Environmental Sciences. Dept. of Geosciences. Organic Geochemistry Group
- Tongji Univ., Shanghai (China). State Key Lab. of Marine Geology
- Publication Date:
- Research Org.:
- Univ. of Nevada, Reno, NV (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1628091
- Grant/Contract Number:
- EE0000716
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 4; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Microbiology; branched GDGTs; hot spring; Great Basin; thermophilic bacteria
Citation Formats
Zhang, Chuanlun L., Wang, Jinxiang, Dodsworth, Jeremy A., Williams, Amanda J., Zhu, Chun, Hinrichs, Kai-Uwe, Zheng, Fengfeng, and Hedlund, Brian P. In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA). United States: N. p., 2013.
Web. doi:10.3389/fmicb.2013.00181.
Zhang, Chuanlun L., Wang, Jinxiang, Dodsworth, Jeremy A., Williams, Amanda J., Zhu, Chun, Hinrichs, Kai-Uwe, Zheng, Fengfeng, & Hedlund, Brian P. In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA). United States. https://doi.org/10.3389/fmicb.2013.00181
Zhang, Chuanlun L., Wang, Jinxiang, Dodsworth, Jeremy A., Williams, Amanda J., Zhu, Chun, Hinrichs, Kai-Uwe, Zheng, Fengfeng, and Hedlund, Brian P. Tue .
"In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)". United States. https://doi.org/10.3389/fmicb.2013.00181. https://www.osti.gov/servlets/purl/1628091.
@article{osti_1628091,
title = {In situ production of branched glycerol dialkyl glycerol tetraethers in a great basin hot spring (USA)},
author = {Zhang, Chuanlun L. and Wang, Jinxiang and Dodsworth, Jeremy A. and Williams, Amanda J. and Zhu, Chun and Hinrichs, Kai-Uwe and Zheng, Fengfeng and Hedlund, Brian P.},
abstractNote = {Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionizationmass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionization-multiple stage mass spectrometry. Our results show multiple lines of evidence for the production of bGDGTs in sediments and cellulolytic enrichments in a hot spring (62–86°C) in the Great Basin (USA). First, in situ cellulolytic enrichment led to an increase in the relative abundance of hydrolysis-derived P-bGDGTs over their C-bGDGT counterparts. Second, the hydrolysisderived P- and C-bGDGT profiles in the hot spring were different from those of the surrounding soil samples; in particular, a monoglycosidic bGDGT Ib containing 13,16- dimethyloctacosane and one cyclopentane moiety was detected in the TLE but it was undetectable in surrounding soil samples even after sample enrichments. Third, previously published 16S rRNA gene pyrotag analysis from the same lignocellulose samples demonstrated the enrichment of thermophiles, rather than mesophiles, and total bGDGT abundance in cellulolytic enrichments correlated with the relative abundance of 16S rRNA gene pyrotags from thermophilic bacteria in the phyla Bacteroidetes, Dictyoglomi, EM3, and OP9 (“Atribacteria”). These observations conclusively demonstrate the production of bGDGTs in this hot spring; however, the identity of organisms that produce bGDGTs in the geothermal environment remains unclear.},
doi = {10.3389/fmicb.2013.00181},
journal = {Frontiers in Microbiology},
number = ,
volume = 4,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2013},
month = {Tue Jan 01 00:00:00 EST 2013}
}
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Works referenced in this record:
Intact polar membrane lipids in prokaryotes and sediments deciphered by high-performance liquid chromatography/electrospray ionization multistage mass spectrometry—new biomarkers for biogeochemistry and microbial ecology
journal, January 2004
- Sturt, Helen F.; Summons, Roger E.; Smith, Kristin
- Rapid Communications in Mass Spectrometry, Vol. 18, Issue 6
Microbiology and geochemistry of great boiling and mud hot springs in the United States Great Basin
journal, February 2009
- Costa, Kyle C.; Navarro, Jason B.; Shock, Everett L.
- Extremophiles, Vol. 13, Issue 3
Branched glycerol dialkyl glycerol tetraethers and paleoenvironmental reconstruction in Zoigê peat sediments during the last 150 years
journal, July 2011
- Zhou, HaoDa; Hu, JianFang; Ming, LiLi
- Chinese Science Bulletin, Vol. 56, Issue 23
A novel proxy for terrestrial organic matter in sediments based on branched and isoprenoid tetraether lipids
journal, July 2004
- Hopmans, Ellen C.; Weijers, Johan W. H.; Schefuß, Enno
- Earth and Planetary Science Letters, Vol. 224, Issue 1-2
Warm arctic continents during the Palaeocene–Eocene thermal maximum
journal, September 2007
- Weijers, Johan W. H.; Schouten, Stefan; Sluijs, Appy
- Earth and Planetary Science Letters, Vol. 261, Issue 1-2
Decoupled warming and monsoon precipitation in East Asia over the last deglaciation
journal, January 2011
- Peterse, Francien; Prins, Maarten A.; Beets, Christiaan J.
- Earth and Planetary Science Letters, Vol. 301, Issue 1-2
Environmental controls on bacterial tetraether membrane lipid distribution in soils
journal, February 2007
- Weijers, Johan W. H.; Schouten, Stefan; van den Donker, Jurgen C.
- Geochimica et Cosmochimica Acta, Vol. 71, Issue 3
Fluxes and distribution of tetraether lipids in an equatorial African lake: Constraints on the application of the TEX86 palaeothermometer and BIT index in lacustrine settings
journal, July 2009
- Damsté, Jaap S. Sinninghe; Ossebaar, Jort; Abbas, Ben
- Geochimica et Cosmochimica Acta, Vol. 73, Issue 14
Absence of seasonal patterns in MBT–CBT indices in mid-latitude soils
journal, June 2011
- Weijers, Johan W. H.; Bernhardt, Beth; Peterse, Francien
- Geochimica et Cosmochimica Acta, Vol. 75, Issue 11
Tracing soil organic carbon in the lower Amazon River and its tributaries using GDGT distributions and bulk organic matter properties
journal, August 2012
- Kim, Jung-Hyun; Zell, Claudia; Moreira-Turcq, Patricia
- Geochimica et Cosmochimica Acta, Vol. 90
Revised calibration of the MBT–CBT paleotemperature proxy based on branched tetraether membrane lipids in surface soils
journal, November 2012
- Peterse, Francien; van der Meer, Jaap; Schouten, Stefan
- Geochimica et Cosmochimica Acta, Vol. 96
Distribution of branched tetraether lipids in geothermally heated soils: Implications for the MBT/CBT temperature proxy
journal, February 2009
- Peterse, Francien; Schouten, Stefan; van der Meer, Jaap
- Organic Geochemistry, Vol. 40, Issue 2
Comparison of instrumental and GDGT-based estimates of sea surface and air temperatures from the Skagerrak
journal, February 2009
- Rueda, Gemma; Rosell-Melé, Antoni; Escala, Marina
- Organic Geochemistry, Vol. 40, Issue 2
Distributions of branched GDGTs in a tropical lake system: Implications for lacustrine application of the MBT/CBT paleoproxy
journal, September 2009
- Tierney, Jessica E.; Russell, James M.
- Organic Geochemistry, Vol. 40, Issue 9
Identification of polar lipid precursors of the ubiquitous branched GDGT orphan lipids in a peat bog in Northern Germany
journal, July 2010
- Liu, Xiao-Lei; Leider, Arne; Gillespie, Aimee
- Organic Geochemistry, Vol. 41, Issue 7
Influence of soil pH on the abundance and distribution of core and intact polar lipid-derived branched GDGTs in soil
journal, October 2010
- Peterse, Francien; Nicol, Graeme W.; Schouten, Stefan
- Organic Geochemistry, Vol. 41, Issue 10
Sources and distributions of tetraether lipids in surface sediments across a large river-dominated continental margin
journal, May 2011
- Zhu, Chun; Weijers, Johan W. H.; Wagner, Thomas
- Organic Geochemistry, Vol. 42, Issue 4
Archaeal and bacterial glycerol dialkyl glycerol tetraethers in sediments from the Eastern Lau Spreading Center, South Pacific Ocean
journal, February 2012
- Hu, Jianfang; Meyers, Philip A.; Chen, Gukui
- Organic Geochemistry, Vol. 43
Distribution of glycerol dialkyl glycerol tetraethers in surface sediments of Lake Qinghai and surrounding soil
journal, June 2012
- Wang, Huanye; Liu, Weiguo; Zhang, Chuanlun L.
- Organic Geochemistry, Vol. 47
The organic geochemistry of glycerol dialkyl glycerol tetraether lipids: A review
journal, January 2013
- Schouten, Stefan; Hopmans, Ellen C.; Sinninghe Damsté, Jaap S.
- Organic Geochemistry, Vol. 54
Microbial glycerol dialkyl glycerol tetraethers from river water and soil near the Three Gorges Dam on the Yangtze River
journal, March 2013
- Yang, Guifang; Zhang, Chuanlun L.; Xie, Shucheng
- Organic Geochemistry, Vol. 56
Archaeal and bacterial glycerol dialkyl glycerol tetraether lipids in chimneys of the Lost City Hydrothermal Field
journal, July 2013
- Lincoln, Sara A.; Bradley, Alexander S.; Newman, Sharon A.
- Organic Geochemistry, Vol. 60
The Phospholipid Ester-linked Fatty Acid Composition of Thermophilic Bacteria
journal, October 1991
- Patel, B. K. C.; Skerratt, J. H.; Nichols, P. D.
- Systematic and Applied Microbiology, Vol. 14, Issue 4
Iso- and Anteiso-Branched Glycerol Diethers of the Thermophilic Anaerobe Thermodesulfotobacterium commune
journal, January 1983
- Langworthy, Thomas A.; Holzer, Günther; Zeikus, J. Gregory
- Systematic and Applied Microbiology, Vol. 4, Issue 1
Formation of Ammonium from Nitrate During Chemolithoautotrophic Growth of the Extremely Thermophilic Bacterium Ammonifex degensii gen. nov. sp. nov.
journal, March 1996
- Huber, R.; Rossnagel, P.; Woese, C. R.
- Systematic and Applied Microbiology, Vol. 19, Issue 1
Assessing past temperature and soil pH estimates from bacterial tetraether membrane lipids: Evidence from the recent lake sediments of Lochnagar, Scotland
journal, March 2010
- Tyler, Jonathan J.; Nederbragt, Alexandra J.; Jones, Vivienne J.
- Journal of Geophysical Research, Vol. 115, Issue G1
Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities
journal, December 2012
- Cole, Jessica K.; Peacock, Joseph P.; Dodsworth, Jeremy A.
- The ISME Journal, Vol. 7, Issue 4
Extended megadroughts in the southwestern United States during Pleistocene interglacials
journal, February 2011
- Fawcett, Peter J.; Werne, Josef P.; Anderson, R. Scott
- Nature, Vol. 470, Issue 7335
Single-cell and metagenomic analyses indicate a fermentative and saccharolytic lifestyle for members of the OP9 lineage
journal, May 2013
- Dodsworth, Jeremy A.; Blainey, Paul C.; Murugapiran, Senthil K.
- Nature Communications, Vol. 4, Issue 1
A new 15,16-dimethyl-30-glyceryloxytriacontanoic acid from lipids of Thermotoga maritima
journal, January 1988
- De Rosa, Mario; Gambacorta, Agata; Huber, Robert
- Journal of the Chemical Society, Chemical Communications, Issue 19
Widespread occurrence of structurally diverse tetraether membrane lipids: Evidence for the ubiquitous presence of low-temperature relatives of hyperthermophiles
journal, December 2000
- Schouten, S.; Hopmans, E. C.; Pancost, R. D.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 26
Lipid Biomarkers, Carbon Isotopes, and Phylogenetic Characterization of Bacteria in California and Nevada Hot Springs
journal, September 2007
- Zhang, Chuanlun L.; Huang, Zhiyong; Li, Yi-Liang
- Geomicrobiology Journal, Vol. 24, Issue 6
Molecular Phylogeny of UncultivatedCrenarchaeotain Great Basin Hot Springs of Moderately Elevated Temperature
journal, September 2007
- Huang, Zhiyong; Hedlund, Brian P.; Wiegel, Juergen
- Geomicrobiology Journal, Vol. 24, Issue 6
Dictyoglomus thermophilum gen. nov., sp. nov., a Chemoorganotrophic, Anaerobic, Thermophilic Bacterium
journal, July 1985
- Saiki, T.; Kobayashi, Y.; Kawagoe, K.
- International Journal of Systematic Bacteriology, Vol. 35, Issue 3
Membrane lipids of mesophilic anaerobic bacteria thriving in peats have typical archaeal traits
journal, April 2006
- Weijers, Johan W. H.; Schouten, Stefan; Hopmans, Ellen C.
- Environmental Microbiology, Vol. 8, Issue 4
Ammonia oxidation, denitrification and dissimilatory nitrate reduction to ammonium in two US Great Basin hot springs with abundant ammonia-oxidizing archaea: N-cycling in Great Basin hot springs
journal, June 2011
- Dodsworth, Jeremy A.; Hungate, Bruce A.; Hedlund, Brian P.
- Environmental Microbiology, Vol. 13, Issue 8
Coupled Thermal and Hydrological Evolution of Tropical Africa over the Last Deglaciation
journal, March 2007
- Weijers, J. W. H.; Schefuss, E.; Schouten, S.
- Science, Vol. 315, Issue 5819
Thermophilic Temperature Optimum for Crenarchaeol Synthesis and Its Implication for Archaeal Evolution
journal, June 2006
- Zhang, Chuanlun L.; Pearson, Ann; Li, Yi-Liang
- Applied and Environmental Microbiology, Vol. 72, Issue 6
13,16-Dimethyl Octacosanedioic Acid ( iso -Diabolic Acid), a Common Membrane-Spanning Lipid of Acidobacteria Subdivisions 1 and 3
journal, April 2011
- Sinninghe Damsté, Jaap S.; Rijpstra, W. Irene C.; Hopmans, Ellen C.
- Applied and Environmental Microbiology, Vol. 77, Issue 12
Archaeal and Bacterial Glycerol Dialkyl Glycerol Tetraether Lipids in Hot Springs of Yellowstone National Park
journal, August 2007
- Schouten, Stefan; van der Meer, Marcel T. J.; Hopmans, Ellen C.
- Applied and Environmental Microbiology, Vol. 73, Issue 19
Global Occurrence of Archaeal amoA Genes in Terrestrial Hot Springs
journal, October 2008
- Zhang, Chuanlun L.; Ye, Qi; Huang, Zhiyong
- Applied and Environmental Microbiology, Vol. 74, Issue 20
Factors Controlling the Distribution of Archaeal Tetraethers in Terrestrial Hot Springs
journal, June 2008
- Pearson, Ann; Pi, Yundan; Zhao, Weidong
- Applied and Environmental Microbiology, Vol. 74, Issue 11
Nonmarine Crenarchaeol in Nevada Hot Springs
journal, September 2004
- Pearson, A.; Huang, Z.; Ingalls, A. E.
- Applied and Environmental Microbiology, Vol. 70, Issue 9
Pyrosequencing Reveals High-Temperature Cellulolytic Microbial Consortia in Great Boiling Spring after In Situ Lignocellulose Enrichment
journal, March 2013
- Peacock, Joseph P.; Cole, Jessica K.; Murugapiran, Senthil K.
- PLoS ONE, Vol. 8, Issue 3
Production of Branched Tetraether Lipids in the Lower Pearl River and Estuary: Effects of Extraction Methods and Impact on bGDGT Proxies
journal, January 2012
- Zhang, Chuanlun L.; Wang, Jinxiang; Wei, Yuli
- Frontiers in Microbiology, Vol. 2
Works referencing / citing this record:
Ether- and Ester-Bound iso -Diabolic Acid and Other Lipids in Members of Acidobacteria Subdivision 4
journal, June 2014
- Sinninghe Damsté, Jaap S.; Rijpstra, W. Irene C.; Hopmans, Ellen C.
- Applied and Environmental Microbiology, Vol. 80, Issue 17
Biotic and Abiotic Imprints on Mg-Rich Stromatolites: Lessons from Lake Salda, SW Turkey
text, January 2020
- Balci, Nurgul; Gunes, Yagmur; Kaiser, Jérôme
- Taylor & Francis
Biotic and Abiotic Imprints on Mg-Rich Stromatolites: Lessons from Lake Salda, SW Turkey
journal, January 2020
- Balci, Nurgul; Gunes, Yagmur; Kaiser, Jérôme
- Geomicrobiology Journal, Vol. 37, Issue 5
Wide distribution of autochthonous branched glycerol dialkyl glycerol tetraethers (bGDGTs) in U.S. Great Basin hot springs
journal, January 2013
- Hedlund, Brian P.; Paraiso, Julienne J.; Williams, Amanda J.
- Frontiers in Microbiology, Vol. 4