Title: Genomic Dissection of Anthracnose ( Colletotrichum sublineolum ) Resistance Response in Sorghum Differential Line SC112-14

Abstract

Sorghum production is expanding to warmer and more humid regions where its production is being limited by multiple fungal pathogens. Anthracnose, caused byColletotrichum sublineolum, is one of the major diseases in these regions, where it can cause yield losses of both grain and biomass. In this study, 114 recombinant inbred lines (RILs) derived from resistant sorghum line SC112-14 were evaluated at four distinct geographic locations in the United States for response to anthracnose. A genome scan using a high-density linkage map of 3,838 single nucleotide polymorphisms (SNPs) detected two loci at 5.25 and 1.18 Mb on chromosomes 5 and 6, respectively, that explain up to 59% and 44% of the observed phenotypic variation. A bin-mapping approach using a subset of 31 highly informative RILs was employed to determine the disease response to inoculation with ten anthracnose pathotypes in the greenhouse. A genome scan showed that the 5.25 Mb region on chromosome 5 is associated with a resistance response to nine pathotypes. Five SNP markers were developed and used to fine map the locus on chromosome 5 by evaluating 1,500 segregating F_2:3progenies. Based on the genotypic and phenotypic analyses of 11 recombinants, the locus was narrowed down to a 470-kb genomicmore » region. Following a genome-wide association study based on 574 accessions previously phenotyped and genotyped, the resistance locus was delimited to a 34-kb genomic interval with five candidate genes. All five candidate genes encode proteins associated with plant immune systems, suggesting they may act in synergy in the resistance response.« less

Authors:

Cruet-Burgos, Clara M. ^[1]; Cuevas, Hugo E.  ^[2];

• Search DOE PAGES for author "Cuevas, Hugo E."
• Search DOE PAGES for ORCID "0000-0001-7272-6933"

Prom, Louis K. ^[3]; Knoll, Joseph E.  ^[4];

• Search DOE PAGES for author "Knoll, Joseph E."
• Search DOE PAGES for ORCID "0000-0002-7477-4039"

Stutts, Lauren R. ^[5]; Vermerris, Wilfred  ^[6]

• Search DOE PAGES for author "Vermerris, Wilfred"
• Search DOE PAGES for ORCID "0000-0002-4582-3436"

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+ Show Author Affiliations

1. USDA-ARS, Mayaguez (Puerto Rico). Tropical Agriculture Research Station; Univ. of Puerto Rico, Mayaguez (Puerto Rico). Dept. of Biology
2. USDA-ARS, Mayaguez (Puerto Rico). Tropical Agriculture Research Station; USDA-ARS, College Station, TX (United States). Southern Plains Agriculture Research Center
3. USDA-ARS, College Station, TX (United States). Southern Plains Agriculture Research Center
4. USDA-ARS, Tifton, GA (United States). Crop Genetics and Breeding Research
5. Univ. of Florida, Gainesville, FL (United States). Graduate Program in Plant and Molecular & Cellular Biology
6. Univ. of Florida, Gainesville, FL (United States). Genetics Inst., Dept. of Microbiology & Cell Science; Univ. of Florida, Gainesville, FL (United States). Florida Center for Renewable Fuels and Chemicals

Publication Date:

Wed Feb 26 00:00:00 EST 2020

Research Org.:

US Dept. of Agriculture (USDA), Washington, DC (United States). Agricultural Research Service

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:

1627963

Grant/Contract Number:  

SC0014171

Resource Type:

Accepted Manuscript

Journal Name:

G3

Additional Journal Information:

Journal Volume: 10; Journal Issue: 4; Journal ID: ISSN 2160-1836

Publisher:

Genetics Society of America

Country of Publication:

United States

Language:

English

Subject:

Genetics & Heredity