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Title: Structural basis of Fusarium myosin I inhibition by phenamacril

Abstract

Fusarium is a genus of filamentous fungi that includes species that cause devastating diseases in major staple crops, such as wheat, maize, rice, and barley, resulting in severe yield losses and mycotoxin contamination of infected grains. Phenamacril is a novel fungicide that is considered environmentally benign due to its exceptional specificity; it inhibits the ATPase activity of the sole class I myosin of only a subset of Fusarium species including the major plant pathogens F. graminearum, F. asiaticum and F. fujikuroi. To understand the underlying mechanisms of inhibition, species specificity, and resistance mutations, we have determined the crystal structure of phenamacril-bound F. graminearum myosin I. Phenamacril binds in the actin-binding cleft in a new allosteric pocket that contains the central residue of the regulatory Switch 2 loop and that is collapsed in the structure of a myosin with closed actin-binding cleft, suggesting that pocket occupancy blocks cleft closure. We have further identified a single, transferable phenamacril-binding residue found exclusively in phenamacril-sensitive myosins to confer phenamacril selectivity.

Authors:
 [1]; ORCiD logo [2];  [3]; ORCiD logo [3];  [3];  [4];  [5];  [3]; ORCiD logo [2]; ORCiD logo [3]
+ Show Author Affiliations
  1. Nanjing Agricultural Univ. of Technology (China). Key Lab. of Pesticide, College of Plant Protection; Van Andel Inst. (VARI), Grand Rapids, MI (United States). Center of Cancer and Cell Biology, Program for Structural Biology
  2. Van Andel Inst. (VARI), Grand Rapids, MI (United States). Center of Cancer and Cell Biology, Program for Structural Biology
  3. Nanjing Agricultural Univ. of Technology (China). Key Lab. of Pesticide, College of Plant Protection
  4. Northwestern Univ., Argonne, IL (United States). Synchrotron Research Center, Life Science Collaborative Access Team
  5. Van Andel Inst. (VARI), Grand Rapids, MI (United States). Center of Cancer and Cell Biology, Program for Structural Biology; Chinese Academy of Sciences (CAS), Shanghai (China)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC); Van Andel Research Institute; National Natural Science Foundation of China (NSFC); Fok Ying-Tong Education Foundation; Six Talent Peaks Project in Jiangsu Province
OSTI Identifier:
1627922
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
PLoS Pathogens
Additional Journal Information:
Journal Volume: 16; Journal Issue: 3; Journal ID: ISSN 1553-7374
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Microbiology; Parasitology; Virology

Citation Formats

Zhou, Yuxin, Zhou, X. Edward, Gong, Yuanping, Zhu, Yuanye, Cao, Xiaoman, Brunzelle, Joseph S., Xu, H. Eric, Zhou, Mingguo, Melcher, Karsten, and Zhang, Feng. Structural basis of Fusarium myosin I inhibition by phenamacril. United States: N. p., 2020. Web. doi:10.1371/journal.ppat.1008323.
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Zhou, Yuxin, Zhou, X. Edward, Gong, Yuanping, Zhu, Yuanye, Cao, Xiaoman, Brunzelle, Joseph S., Xu, H. Eric, Zhou, Mingguo, Melcher, Karsten, & Zhang, Feng. Structural basis of Fusarium myosin I inhibition by phenamacril. United States. https://doi.org/10.1371/journal.ppat.1008323
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Zhou, Yuxin, Zhou, X. Edward, Gong, Yuanping, Zhu, Yuanye, Cao, Xiaoman, Brunzelle, Joseph S., Xu, H. Eric, Zhou, Mingguo, Melcher, Karsten, and Zhang, Feng. Thu . "Structural basis of Fusarium myosin I inhibition by phenamacril". United States. https://doi.org/10.1371/journal.ppat.1008323. https://www.osti.gov/servlets/purl/1627922.
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@article{osti_1627922,
title = {Structural basis of Fusarium myosin I inhibition by phenamacril},
author = {Zhou, Yuxin and Zhou, X. Edward and Gong, Yuanping and Zhu, Yuanye and Cao, Xiaoman and Brunzelle, Joseph S. and Xu, H. Eric and Zhou, Mingguo and Melcher, Karsten and Zhang, Feng},
abstractNote = {Fusarium is a genus of filamentous fungi that includes species that cause devastating diseases in major staple crops, such as wheat, maize, rice, and barley, resulting in severe yield losses and mycotoxin contamination of infected grains. Phenamacril is a novel fungicide that is considered environmentally benign due to its exceptional specificity; it inhibits the ATPase activity of the sole class I myosin of only a subset of Fusarium species including the major plant pathogens F. graminearum, F. asiaticum and F. fujikuroi. To understand the underlying mechanisms of inhibition, species specificity, and resistance mutations, we have determined the crystal structure of phenamacril-bound F. graminearum myosin I. Phenamacril binds in the actin-binding cleft in a new allosteric pocket that contains the central residue of the regulatory Switch 2 loop and that is collapsed in the structure of a myosin with closed actin-binding cleft, suggesting that pocket occupancy blocks cleft closure. We have further identified a single, transferable phenamacril-binding residue found exclusively in phenamacril-sensitive myosins to confer phenamacril selectivity.},
doi = {10.1371/journal.ppat.1008323},
journal = {PLoS Pathogens},
number = 3,
volume = 16,
place = {United States},
year = {2020},
month = {3}
}
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https://doi.org/10.1371/journal.ppat.1008323
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Works referenced in this record:

Heading for disaster: Fusarium graminearum on cereal crops: Fusarium graminearum on cereal crops
journal, November 2004

Community Ecology of Fungal Pathogens Causing Wheat Head Blight
journal, September 2009

New insights into myosin evolution and classification
journal, February 2006

  • Foth, B. J.; Goedecke, M. C.; Soldati, D.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 10
  • DOI: 10.1073/pnas.0506307103

Whole-genome sequencing reveals that mutations in myosin-5 confer resistance to the fungicide phenamacril in Fusarium graminearum
journal, February 2015

  • Zheng, Zhitian; Hou, Yiping; Cai, Yiqiang
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep08248

Cargo transport: molecular motors navigate a complex cytoskeleton
journal, February 2008

  • Ross, Jennifer L.; Ali, M. Yusuf; Warshaw, David M.
  • Current Opinion in Cell Biology, Vol. 20, Issue 1
  • DOI: 10.1016/j.ceb.2007.11.006

The myosin superfamily at a glance
journal, April 2012

  • Hartman, M. A.; Spudich, J. A.
  • Journal of Cell Science, Vol. 125, Issue 7
  • DOI: 10.1242/jcs.094300

Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1
journal, July 1993

  • Schröder, Rasmus R.; Manstein, Dietmar J.; Jahn, Werner
  • Nature, Vol. 364, Issue 6433
  • DOI: 10.1038/364171a0

The neck region of the myosin motor domain acts as a lever arm to generate movement.
journal, April 1996

  • Uyeda, T. Q.; Abramson, P. D.; Spudich, J. A.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 9
  • DOI: 10.1073/pnas.93.9.4459

How Actin Initiates the Motor Activity of Myosin
journal, May 2015

How Myosin Generates Force on Actin Filaments
journal, December 2016

Single molecule mechanics resolves the earliest events in force generation by cardiac myosin
journal, September 2019

  • Woody, Michael S.; Winkelmann, Donald A.; Capitanio, Marco
  • eLife, Vol. 8
  • DOI: 10.7554/eLife.49266

Myosin VI Rewrites the Rules for Myosin Motors
journal, May 2010

Structural and Functional Insights into the Myosin Motor Mechanism
journal, April 2010

Prolonged cross-bridge binding triggers muscle dysfunction in a Drosophila model of myosin-based hypertrophic cardiomyopathy
journal, August 2018

  • Kronert, William A.; Bell, Kaylyn M.; Viswanathan, Meera C.
  • eLife, Vol. 7
  • DOI: 10.7554/eLife.38064

Mutant p53–associated myosin-X upregulation promotes breast cancer invasion and metastasis
journal, February 2014

  • Arjonen, Antti; Kaukonen, Riina; Mattila, Elina
  • Journal of Clinical Investigation, Vol. 124, Issue 3
  • DOI: 10.1172/JCI67280

Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
journal, March 2003

A small-molecule inhibitor of skeletal muscle myosin II
journal, December 2001

  • Cheung, A.; Dantzig, J. A.; Hollingworth, S.
  • Nature Cell Biology, Vol. 4, Issue 1
  • DOI: 10.1038/ncb734

A small-molecule inhibitor of sarcomere contractility suppresses hypertrophic cardiomyopathy in mice
journal, February 2016

The mechanism of pentabromopseudilin inhibition of myosin motor activity
journal, January 2009

  • Fedorov, Roman; Böhl, Markus; Tsiavaliaris, Georgios
  • Nature Structural & Molecular Biology, Vol. 16, Issue 1
  • DOI: 10.1038/nsmb.1542

A Myosin V Inhibitor Based on Privileged Chemical Scaffolds
journal, September 2010

  • Islam, Kabirul; Chin, Harvey F.; Olivares, Adrian O.
  • Angewandte Chemie International Edition, Vol. 49, Issue 45
  • DOI: 10.1002/anie.201004026

Kinetic properties and small-molecule inhibition of human myosin-6
journal, July 2012

A small molecule species specifically inhibits F usarium myosin I: JS399-19 inhibits fungal myosin I
journal, January 2015

JS399-19, a new fungicide against wheat scab
journal, January 2008

FoMyo5 motor domain substitutions (Val151 to Ala and Ser418 to Thr) cause natural resistance to fungicide phenamacril in Fusarium oxysporum
journal, May 2018

Resistance mechanism of Fusarium fujikuroi to phenamacril in the field
journal, November 2017

  • Hou, Yi‐Ping; Qu, Xiang‐Pu; Mao, Xue‐Wei
  • Pest Management Science, Vol. 74, Issue 3
  • DOI: 10.1002/ps.4742

Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling
journal, December 2014

  • Lu, Qing; Li, Jianchao; Ye, Fei
  • Nature Structural & Molecular Biology, Vol. 22, Issue 1
  • DOI: 10.1038/nsmb.2923

Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor
journal, September 2016

  • Shen, Mei; Zhang, Ning; Zheng, Sanduo
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 40
  • DOI: 10.1073/pnas.1607702113

Crystal structure of the motor domain of a class-I myosin
journal, June 2002

The structural basis of blebbistatin inhibition and specificity for myosin II
journal, March 2005

  • Allingham, John S.; Smith, Robert; Rayment, Ivan
  • Nature Structural & Molecular Biology, Vol. 12, Issue 4
  • DOI: 10.1038/nsmb908

Inhibition of Myosin ATPase Activity by Halogenated Pseudilins: A Structure–Activity Study
journal, June 2011

  • Preller, Matthias; Chinthalapudi, Krishna; Martin, René
  • Journal of Medicinal Chemistry, Vol. 54, Issue 11
  • DOI: 10.1021/jm200259f

Structural basis for drug-induced allosteric changes to human β-cardiac myosin motor activity
journal, August 2015

  • Winkelmann, Donald A.; Forgacs, Eva; Miller, Matthew T.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms8974

Three myosin V structures delineate essential features of chemo-mechanical transduction
journal, October 2004

  • Coureux, Pierre-Damien; Sweeney, H. Lee; Houdusse, Anne
  • The EMBO Journal, Vol. 23, Issue 23
  • DOI: 10.1038/sj.emboj.7600458

A structural state of the myosin V motor without bound nucleotide
journal, September 2003

  • Coureux, Pierre-Damien; Wells, Amber L.; Ménétrey, Julie
  • Nature, Vol. 425, Issue 6956
  • DOI: 10.1038/nature01927

Rigor-like Structures from Muscle Myosins Reveal Key Mechanical Elements in the Transduction Pathways of This Allosteric Motor
journal, May 2007

Processive Steps in the Reverse Direction Require Uncoupling of the Lead Head Lever Arm of Myosin VI
journal, October 2012

The structure of the myosin VI motor reveals the mechanism of directionality reversal
journal, June 2005

  • Ménétrey, Julie; Bahloul, Amel; Wells, Amber L.
  • Nature, Vol. 435, Issue 7043
  • DOI: 10.1038/nature03592

Insights into the Importance of Hydrogen Bonding in the γ-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Serine 236,
journal, June 2010

  • Frye, Jeremiah J.; Klenchin, Vadim A.; Bagshaw, Clive R.
  • Biochemistry, Vol. 49, Issue 23
  • DOI: 10.1021/bi1001344

Filamentous fungal infections of the cornea: a global overview of epidemiology and drug sensitivity
journal, February 2015

  • Kredics, László; Narendran, Venkatapathy; Shobana, Coimbatore Subramanian
  • Mycoses, Vol. 58, Issue 4
  • DOI: 10.1111/myc.12306

Identification and Characterization of Fusarium proliferatum, a New Species of Fungi that Cause Fungal Keratitis
journal, March 2018

MyD88 Regulation of Fusarium Keratitis Is Dependent on TLR4 and IL-1R1 but Not TLR2
journal, June 2008

Fungal keratitis: An overview of clinical and laboratory aspects
journal, July 2018

  • Mahmoudi, Shahram; Masoomi, Ahmad; Ahmadikia, Kazem
  • Mycoses, Vol. 61, Issue 12
  • DOI: 10.1111/myc.12822

Spectrum of fungal keratitis in central China
journal, November 2009

XDS
journal, January 2010

  • Kabsch, Wolfgang
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2
  • DOI: 10.1107/S0907444909047337

Crystal Structure of Human Myosin 1c—The Motor in GLUT4 Exocytosis: Implications for Ca2+ Regulation and 14-3-3 Binding
journal, May 2014

  • Münnich, Stefan; Taft, Manuel H.; Manstein, Dietmar J.
  • Journal of Molecular Biology, Vol. 426, Issue 10
  • DOI: 10.1016/j.jmb.2014.03.004

Structural insights into the potency of SK channel positive modulators
journal, December 2017

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

PHENIX: a comprehensive Python-based system for macromolecular structure solution
journal, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221
  • DOI: 10.1107/S0907444909052925

Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi
journal, November 2004

  • Yu, Jae-Hyuk; Hamari, Zsuzsanna; Han, Kap-Hoon
  • Fungal Genetics and Biology, Vol. 41, Issue 11
  • DOI: 10.1016/j.fgb.2004.08.001

A Myosin V Inhibitor Based on Privileged Chemical Scaffolds
journal, September 2010

  • Islam, Kabirul; Chin, Harvey F.; Olivares, Adrian O.
  • Angewandte Chemie International Edition, Vol. 49, Issue 45
  • DOI: 10.1002/anie.201004026

Resistance mechanism of Fusarium fujikuroi to phenamacril in the field
journal, November 2017

  • Hou, Yi‐Ping; Qu, Xiang‐Pu; Mao, Xue‐Wei
  • Pest Management Science, Vol. 74, Issue 3
  • DOI: 10.1002/ps.4742

Cargo transport: molecular motors navigate a complex cytoskeleton
journal, February 2008

  • Ross, Jennifer L.; Ali, M. Yusuf; Warshaw, David M.
  • Current Opinion in Cell Biology, Vol. 20, Issue 1
  • DOI: 10.1016/j.ceb.2007.11.006

Kinetic properties and small-molecule inhibition of human myosin-6
journal, July 2012

Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi
journal, November 2004

  • Yu, Jae-Hyuk; Hamari, Zsuzsanna; Han, Kap-Hoon
  • Fungal Genetics and Biology, Vol. 41, Issue 11
  • DOI: 10.1016/j.fgb.2004.08.001

Crystal Structure of Human Myosin 1c—The Motor in GLUT4 Exocytosis: Implications for Ca2+ Regulation and 14-3-3 Binding
journal, May 2014

  • Münnich, Stefan; Taft, Manuel H.; Manstein, Dietmar J.
  • Journal of Molecular Biology, Vol. 426, Issue 10
  • DOI: 10.1016/j.jmb.2014.03.004

Processive Steps in the Reverse Direction Require Uncoupling of the Lead Head Lever Arm of Myosin VI
journal, October 2012

Rigor-like Structures from Muscle Myosins Reveal Key Mechanical Elements in the Transduction Pathways of This Allosteric Motor
journal, May 2007

How Myosin Generates Force on Actin Filaments
journal, December 2016

Activity of the Fungicide JS399-19 Against Fusarium Head Blight of Wheat and the Risk of Resistance
journal, December 2011

Inhibition of Myosin ATPase Activity by Halogenated Pseudilins: A Structure–Activity Study
journal, June 2011

  • Preller, Matthias; Chinthalapudi, Krishna; Martin, René
  • Journal of Medicinal Chemistry, Vol. 54, Issue 11
  • DOI: 10.1021/jm200259f

Three-dimensional atomic model of F-actin decorated with Dictyostelium myosin S1
journal, July 1993

  • Schröder, Rasmus R.; Manstein, Dietmar J.; Jahn, Werner
  • Nature, Vol. 364, Issue 6433
  • DOI: 10.1038/364171a0

The structure of the myosin VI motor reveals the mechanism of directionality reversal
journal, June 2005

  • Ménétrey, Julie; Bahloul, Amel; Wells, Amber L.
  • Nature, Vol. 435, Issue 7043
  • DOI: 10.1038/nature03592

A small-molecule inhibitor of skeletal muscle myosin II
journal, December 2001

  • Cheung, A.; Dantzig, J. A.; Hollingworth, S.
  • Nature Cell Biology, Vol. 4, Issue 1
  • DOI: 10.1038/ncb734

Structural basis for drug-induced allosteric changes to human β-cardiac myosin motor activity
journal, August 2015

  • Winkelmann, Donald A.; Forgacs, Eva; Miller, Matthew T.
  • Nature Communications, Vol. 6, Issue 1
  • DOI: 10.1038/ncomms8974

Structure of myosin-1c tail bound to calmodulin provides insights into calcium-mediated conformational coupling
journal, December 2014

  • Lu, Qing; Li, Jianchao; Ye, Fei
  • Nature Structural & Molecular Biology, Vol. 22, Issue 1
  • DOI: 10.1038/nsmb.2923

The structural basis of blebbistatin inhibition and specificity for myosin II
journal, March 2005

  • Allingham, John S.; Smith, Robert; Rayment, Ivan
  • Nature Structural & Molecular Biology, Vol. 12, Issue 4
  • DOI: 10.1038/nsmb908

Identification and Characterization of Fusarium proliferatum, a New Species of Fungi that Cause Fungal Keratitis
journal, March 2018

Whole-genome sequencing reveals that mutations in myosin-5 confer resistance to the fungicide phenamacril in Fusarium graminearum
journal, February 2015

  • Zheng, Zhitian; Hou, Yiping; Cai, Yiqiang
  • Scientific Reports, Vol. 5, Issue 1
  • DOI: 10.1038/srep08248

New insights into myosin evolution and classification
journal, February 2006

  • Foth, B. J.; Goedecke, M. C.; Soldati, D.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 10
  • DOI: 10.1073/pnas.0506307103

Calmodulin in complex with the first IQ motif of myosin-5a functions as an intact calcium sensor
journal, September 2016

  • Shen, Mei; Zhang, Ning; Zheng, Sanduo
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 40
  • DOI: 10.1073/pnas.1607702113

The neck region of the myosin motor domain acts as a lever arm to generate movement.
journal, April 1996

  • Uyeda, T. Q.; Abramson, P. D.; Spudich, J. A.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 9
  • DOI: 10.1073/pnas.93.9.4459

Mechanism and Specificity of Pentachloropseudilin-mediated Inhibition of Myosin Motor Activity
journal, June 2011

  • Chinthalapudi, Krishna; Taft, Manuel H.; Martin, René
  • Journal of Biological Chemistry, Vol. 286, Issue 34
  • DOI: 10.1074/jbc.m111.239210

A small-molecule modulator of cardiac myosin acts on multiple stages of the myosin chemomechanical cycle
journal, August 2017

  • Kawas, Raja F.; Anderson, Robert L.; Ingle, Sadie R. Bartholomew
  • Journal of Biological Chemistry, Vol. 292, Issue 40
  • DOI: 10.1074/jbc.m117.776815

Mechanism of Blebbistatin Inhibition of Myosin II
journal, June 2004

  • Kovács, Mihály; Tóth, Judit; Hetényi, Csaba
  • Journal of Biological Chemistry, Vol. 279, Issue 34
  • DOI: 10.1074/jbc.m405319200

Crystal structure of the motor domain of a class-I myosin
journal, June 2002

Butanedione Monoxime Suppresses Contraction and ATPase Activity of Rabbit Skeletal Muscle1
journal, April 1989

Genotypes and Characteristics of Phenamacril-Resistant Mutants in Fusarium asiaticum
journal, August 2016

Heading for disaster: Fusarium graminearum on cereal crops: Fusarium graminearum on cereal crops
journal, November 2004

Spectrum of fungal keratitis in central China
journal, November 2009

Fungal keratitis: An overview of clinical and laboratory aspects
journal, July 2018

  • Mahmoudi, Shahram; Masoomi, Ahmad; Ahmadikia, Kazem
  • Mycoses, Vol. 61, Issue 12
  • DOI: 10.1111/myc.12822

Dissecting Temporal and Spatial Control of Cytokinesis with a Myosin II Inhibitor
journal, March 2003

A small-molecule inhibitor of sarcomere contractility suppresses hypertrophic cardiomyopathy in mice
journal, February 2016

Community Ecology of Fungal Pathogens Causing Wheat Head Blight
journal, September 2009

Structural and Functional Insights into the Myosin Motor Mechanism
journal, April 2010

PHENIX: a comprehensive Python-based system for macromolecular structure solution.
text, January 2010

  • Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor
  • Apollo - University of Cambridge Repository
  • DOI: 10.17863/cam.45787

The mechanism of pentabromopseudilin inhibition of myosin motor activity
text, January 2009

  • Fedorov, R.; Bohl, M.; Tsiavaliaris, G.
  • Deutsches Elektronen-Synchrotron, DESY, Hamburg
  • DOI: 10.3204/phppubdb-11987

MyD88 Regulation of Fusarium Keratitis Is Dependent on TLR4 and IL-1R1 but Not TLR2
journal, June 2008

Too much of a good thing
journal, September 2018

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