Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently infected human hematopoietic cell lines
Abstract
Zika virus (ZIKV), an arthropod-borne virus, has emerged as a major human pathogen. Prolonged or persistent ZIKV infection of human cells and tissues may serve as a reservoir for the virus and present serious challenges to the safety of public health. Human hematopoietic cell lines with different developmental properties revealed differences in susceptibility and outcomes to ZIKV infection. In three separate studies involving the prototypic MR 766 ZIKV strain and the human monocytic leukemia U937 cell line, ZIKV initially developed only a low-grade infection at a slow rate. After continuous culture for several months, persistently ZIKV-infected cell lines were observed with most, if not all, cells testing positive for ZIKV antigen. The infected cultures produced ZIKV RNA (v-RNA) and infectious ZIKVs persistently (“persistent ZIKVs”) with distinct infectivity and pathogenicity when tested using various kinds of host cells. When the genomes of ZIKVs from the three persistently infected cell lines were compared with the genome of the prototypic MR 766 ZIKV strain, distinct sets of mutations specific to each cell line were found. Significantly, all three “persistent ZIKVs” were capable of infecting fresh U937 cells with high efficiency at rapid rates, resulting in the development of a new set of persistentlymore »
- Authors:
-
[1];
[1]
- U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Biologics Evaluation and Research. Office of Tissues and Advanced Therapies. Division of Cellular and Gene Therapies. Tissue Microbiology Lab.
- U.S. Food and Drug Administration (FDA), Silver Spring, MD (United States). Center for Biologics Evaluation and Research. Office of Vaccines Research and Review. Division of Viral Products. Lab of Pediatric and Respiratory Viral Diseases
- Publication Date:
- Research Org.:
- Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1627866
- Grant/Contract Number:
- SC0014664
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 9; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics
Citation Formats
Li, Bingjie, Liao, Hsiao-Mei, Liu, Hebing, Tsai, Shien, Zhang, Jing, Hung, Guo-Chiuan, Chin, Pei-Ju, Gao, Yamei, and Lo, Shyh-Ching. Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently infected human hematopoietic cell lines. United States: N. p., 2018.
Web. doi:10.1371/journal.pone.0203331.
Li, Bingjie, Liao, Hsiao-Mei, Liu, Hebing, Tsai, Shien, Zhang, Jing, Hung, Guo-Chiuan, Chin, Pei-Ju, Gao, Yamei, & Lo, Shyh-Ching. Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently infected human hematopoietic cell lines. United States. https://doi.org/10.1371/journal.pone.0203331
Li, Bingjie, Liao, Hsiao-Mei, Liu, Hebing, Tsai, Shien, Zhang, Jing, Hung, Guo-Chiuan, Chin, Pei-Ju, Gao, Yamei, and Lo, Shyh-Ching. Fri .
"Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently infected human hematopoietic cell lines". United States. https://doi.org/10.1371/journal.pone.0203331. https://www.osti.gov/servlets/purl/1627866.
@article{osti_1627866,
title = {Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently infected human hematopoietic cell lines},
author = {Li, Bingjie and Liao, Hsiao-Mei and Liu, Hebing and Tsai, Shien and Zhang, Jing and Hung, Guo-Chiuan and Chin, Pei-Ju and Gao, Yamei and Lo, Shyh-Ching},
abstractNote = {Zika virus (ZIKV), an arthropod-borne virus, has emerged as a major human pathogen. Prolonged or persistent ZIKV infection of human cells and tissues may serve as a reservoir for the virus and present serious challenges to the safety of public health. Human hematopoietic cell lines with different developmental properties revealed differences in susceptibility and outcomes to ZIKV infection. In three separate studies involving the prototypic MR 766 ZIKV strain and the human monocytic leukemia U937 cell line, ZIKV initially developed only a low-grade infection at a slow rate. After continuous culture for several months, persistently ZIKV-infected cell lines were observed with most, if not all, cells testing positive for ZIKV antigen. The infected cultures produced ZIKV RNA (v-RNA) and infectious ZIKVs persistently (“persistent ZIKVs”) with distinct infectivity and pathogenicity when tested using various kinds of host cells. When the genomes of ZIKVs from the three persistently infected cell lines were compared with the genome of the prototypic MR 766 ZIKV strain, distinct sets of mutations specific to each cell line were found. Significantly, all three “persistent ZIKVs” were capable of infecting fresh U937 cells with high efficiency at rapid rates, resulting in the development of a new set of persistently ZIKV-infected U937 cell lines. The genomes of ZIKVs from the new set of persistently ZIKV-infected U937 cell lines were further analyzed for their different mutations. The 2nd generation of persistent ZIKVs continued to possess most of the distinct sets of mutations specific to the respective 1st generation of persistent ZIKVs. We anticipate that the study will contribute to the understanding of the fundamental biology of adaptive mutations and selection during viral persistence. The persistently ZIKVinfected human cell lines that we developed will also be useful to investigate critical molecular pathways of ZIKV persistence and to study drugs or countermeasures against ZIKV infections and transmission.},
doi = {10.1371/journal.pone.0203331},
journal = {PLoS ONE},
number = 9,
volume = 13,
place = {United States},
year = {Fri Sep 07 00:00:00 EDT 2018},
month = {Fri Sep 07 00:00:00 EDT 2018}
}
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Works referencing / citing this record:
Comparative Genomics, Infectivity and Cytopathogenicity of American Isolates of Zika Virus that Developed Persistent Infections in Human Embryonic Kidney (HEK293) Cells
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dataset, January 2019
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Data from: Comparative genomics, infectivity and cytopathogenicity of Zika viruses produced by acutely and persistently Zika virus-infected human hematopoietic cell lines
dataset, January 2019
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- Viruses, Vol. 10, Issue 11