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Title: Complexity and Variability of Gut Commensal Microbiota in Polyphagous Lepidopteran Larvae

Abstract

Background: The gut of most insects harbours nonpathogenic microorganisms. Recent work suggests that gut microbiota not only provide nutrients, but also involve in the development and maintenance of the host immune system. However, the complexity, dynamics and types of interactions between the insect hosts and their gut microbiota are far from being well understood. Methods/Principal Findings: To determine the composition of the gut microbiota of two lepidopteran pests, Spodoptera littoralis and Helicoverpa armigera, we applied cultivation-independent techniques based on 16S rRNA gene sequencing and microarray. The two insect species were very similar regarding high abundant bacterial families. Different bacteria colonize different niches within the gut. A core community, consisting of Enterococci, Lactobacilli, Clostridia, etc. was revealed in the insect larvae. These bacteria are constantly present in the digestion tract at relatively high frequency despite that developmental stage and diet had a great impact on shaping the bacterial communities. Some low-abundant species might become dominant upon loading external disturbances; the core community, however, did not change significantly. Clearly the insect gut selects for particular bacterial phylotypes. Conclusions: Because of their importance as agricultural pests, phytophagous Lepidopterans are widely used as experimental models in ecological and physiological studies. Our results demonstrated thatmore » a core microbial community exists in the insect gut, which may contribute to the host physiology. Host physiology and food, nevertheless, significantly influence some fringe bacterial species in the gut. The gut microbiota might also serve as a reservoir of microorganisms for ever-changing environments. Understanding these interactions might pave the way for developing novel pest control strategies.« less

Authors:
 [1];  [2];  [2];  [1];  [1];  [1];  [3];  [4];  [2];  [1]
  1. Max Planck Inst. for Chemical Ecology, Jena (Germany). Dept. of Bioorganic Chemistry
  2. Max Planck Inst. for Chemical Ecology, Jena (Germany). Dept. of Entomology
  3. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Center for Environmental Biology and Molecular Microbial Ecology
  4. Friedrich Schiller University of Jena (Germany). The Univ. Hospital. Centre of Electron Microscopy
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
OSTI Identifier:
1627518
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 7; Journal Issue: 7; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Tang, Xiaoshu, Freitak, Dalial, Vogel, Heiko, Ping, Liyan, Shao, Yongqi, Cordero, Erika Arias, Andersen, Gary, Westermann, Martin, Heckel, David G., and Boland, Wilhelm. Complexity and Variability of Gut Commensal Microbiota in Polyphagous Lepidopteran Larvae. United States: N. p., 2012. Web. https://doi.org/10.1371/journal.pone.0036978.
Tang, Xiaoshu, Freitak, Dalial, Vogel, Heiko, Ping, Liyan, Shao, Yongqi, Cordero, Erika Arias, Andersen, Gary, Westermann, Martin, Heckel, David G., & Boland, Wilhelm. Complexity and Variability of Gut Commensal Microbiota in Polyphagous Lepidopteran Larvae. United States. https://doi.org/10.1371/journal.pone.0036978
Tang, Xiaoshu, Freitak, Dalial, Vogel, Heiko, Ping, Liyan, Shao, Yongqi, Cordero, Erika Arias, Andersen, Gary, Westermann, Martin, Heckel, David G., and Boland, Wilhelm. Tue . "Complexity and Variability of Gut Commensal Microbiota in Polyphagous Lepidopteran Larvae". United States. https://doi.org/10.1371/journal.pone.0036978. https://www.osti.gov/servlets/purl/1627518.
@article{osti_1627518,
title = {Complexity and Variability of Gut Commensal Microbiota in Polyphagous Lepidopteran Larvae},
author = {Tang, Xiaoshu and Freitak, Dalial and Vogel, Heiko and Ping, Liyan and Shao, Yongqi and Cordero, Erika Arias and Andersen, Gary and Westermann, Martin and Heckel, David G. and Boland, Wilhelm},
abstractNote = {Background: The gut of most insects harbours nonpathogenic microorganisms. Recent work suggests that gut microbiota not only provide nutrients, but also involve in the development and maintenance of the host immune system. However, the complexity, dynamics and types of interactions between the insect hosts and their gut microbiota are far from being well understood. Methods/Principal Findings: To determine the composition of the gut microbiota of two lepidopteran pests, Spodoptera littoralis and Helicoverpa armigera, we applied cultivation-independent techniques based on 16S rRNA gene sequencing and microarray. The two insect species were very similar regarding high abundant bacterial families. Different bacteria colonize different niches within the gut. A core community, consisting of Enterococci, Lactobacilli, Clostridia, etc. was revealed in the insect larvae. These bacteria are constantly present in the digestion tract at relatively high frequency despite that developmental stage and diet had a great impact on shaping the bacterial communities. Some low-abundant species might become dominant upon loading external disturbances; the core community, however, did not change significantly. Clearly the insect gut selects for particular bacterial phylotypes. Conclusions: Because of their importance as agricultural pests, phytophagous Lepidopterans are widely used as experimental models in ecological and physiological studies. Our results demonstrated that a core microbial community exists in the insect gut, which may contribute to the host physiology. Host physiology and food, nevertheless, significantly influence some fringe bacterial species in the gut. The gut microbiota might also serve as a reservoir of microorganisms for ever-changing environments. Understanding these interactions might pave the way for developing novel pest control strategies.},
doi = {10.1371/journal.pone.0036978},
journal = {PLoS ONE},
number = 7,
volume = 7,
place = {United States},
year = {2012},
month = {7}
}

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