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Title: Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress

Abstract

Heat shock proteins (Hsps) are among the most widely distributed and evolutionary conserved proteins. Hsps are essential regulators of diverse constitutive metabolic processes and are markedly upregulated during stress. A 62 kDa Hsp (Hsp60) of Histoplasma capsulatum (Hc) is an immunodominant antigen and the major surface ligand to CR3 receptors on macrophages. However little is known about the function of this protein within the fungus. We characterized Hc Hsp60- protein interactions under different temperature to gain insights of its additional functions oncell wall dynamism, heat stress and pathogenesis. We conducted co-immunoprecipitations with antibodies to Hc Hsp60 using cytoplasmic and cell wall extracts. Interacting proteins were identified by shotgun proteomics. For the cell wall, 84 common interactions were identified among the 3 growth conditions, including proteins involved in heat-shock response, sugar and amino acid/ protein metabolism and cell signaling. Unique interactions were found at each temperature [30uC (81 proteins), 37uC (14) and 37/40uC (47)]. There were fewer unique interactions in cytoplasm [30uC (6), 37uC (25) and 37/40uC (39)] and four common interactions, including additional Hsps and other known virulence factors. These results show the complexity of Hsp60 function and provide insights into Hc biology, which may lead to new avenues formore » the management of histoplasmosis.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [1]
  1. Yeshiva Univ., Bronx, NY (United States). Albert Einstein College of Medicine. Dept. of Medicine. Division of Infectious Diseases; Yeshiva Univ., Bronx, NY (United States). Albert Einstein College of Medicine. Dept. of Microbiology and Immunology
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. Heart Inst. (InCor), Sao Paulo (Brazil). Lab. of Genetics and Molecular Cardiology. Group of Computational Biology
  4. Yeshiva Univ., Bronx, NY (United States). Albert Einstein College of Medicine. Dept. of Microbiology and Immunology
  5. Universidade Federal do Rio de Janeiro (Brazil). Instituto de Microbiologia Professor Paulo de Goes. Laboratorio de Estudos Integrados em Bioquımica Microbiana
  6. Univ. of Texas at El Paso, TX (United States). The Border Biomedical Research Center. Dept. of Biological Sciences
Publication Date:
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
OSTI Identifier:
1627436
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 6; Journal Issue: 2; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Guimarães, Allan Jefferson, Nakayasu, Ernesto S., Sobreira, Tiago J. P., Cordero, Radames J. B., Nimrichter, Leonardo, Almeida, Igor C., and Nosanchuk, Joshua Daniel. Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress. United States: N. p., 2011. Web. https://doi.org/10.1371/journal.pone.0014660.
Guimarães, Allan Jefferson, Nakayasu, Ernesto S., Sobreira, Tiago J. P., Cordero, Radames J. B., Nimrichter, Leonardo, Almeida, Igor C., & Nosanchuk, Joshua Daniel. Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress. United States. https://doi.org/10.1371/journal.pone.0014660
Guimarães, Allan Jefferson, Nakayasu, Ernesto S., Sobreira, Tiago J. P., Cordero, Radames J. B., Nimrichter, Leonardo, Almeida, Igor C., and Nosanchuk, Joshua Daniel. Thu . "Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress". United States. https://doi.org/10.1371/journal.pone.0014660. https://www.osti.gov/servlets/purl/1627436.
@article{osti_1627436,
title = {Histoplasma capsulatum Heat-Shock 60 Orchestrates the Adaptation of the Fungus to Temperature Stress},
author = {Guimarães, Allan Jefferson and Nakayasu, Ernesto S. and Sobreira, Tiago J. P. and Cordero, Radames J. B. and Nimrichter, Leonardo and Almeida, Igor C. and Nosanchuk, Joshua Daniel},
abstractNote = {Heat shock proteins (Hsps) are among the most widely distributed and evolutionary conserved proteins. Hsps are essential regulators of diverse constitutive metabolic processes and are markedly upregulated during stress. A 62 kDa Hsp (Hsp60) of Histoplasma capsulatum (Hc) is an immunodominant antigen and the major surface ligand to CR3 receptors on macrophages. However little is known about the function of this protein within the fungus. We characterized Hc Hsp60- protein interactions under different temperature to gain insights of its additional functions oncell wall dynamism, heat stress and pathogenesis. We conducted co-immunoprecipitations with antibodies to Hc Hsp60 using cytoplasmic and cell wall extracts. Interacting proteins were identified by shotgun proteomics. For the cell wall, 84 common interactions were identified among the 3 growth conditions, including proteins involved in heat-shock response, sugar and amino acid/ protein metabolism and cell signaling. Unique interactions were found at each temperature [30uC (81 proteins), 37uC (14) and 37/40uC (47)]. There were fewer unique interactions in cytoplasm [30uC (6), 37uC (25) and 37/40uC (39)] and four common interactions, including additional Hsps and other known virulence factors. These results show the complexity of Hsp60 function and provide insights into Hc biology, which may lead to new avenues for the management of histoplasmosis.},
doi = {10.1371/journal.pone.0014660},
journal = {PLoS ONE},
number = 2,
volume = 6,
place = {United States},
year = {2011},
month = {2}
}

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