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Title: Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block

Abstract

Background: Understanding the interactions between ion channels and blockers remains an important goal that has implications for delineating the basic mechanisms of ion channel function and for the discovery and development of ion channel directed drugs. Methodology/Principal Findings: We used genetic selection methods to probe the interaction of two ion channel blockers, barium and amantadine, with the miniature viral potassium channel Kcv. Selection for Kcv mutants that were resistant to either blocker identified a mutant bearing multiple changes that was resistant to both. Implementation of a PCR shuffling and backcrossing procedure uncovered that the blocker resistance could be attributed to a single change, T63S, at a position that is likely to form the binding site for the inner ion in the selectivity filter (site 4). A combination of electrophysiological and biochemical assays revealed a distinct difference in the ability of the mutant channel to interact with the blockers. Studies of the analogous mutation in the mammalian inward rectifier Kir2.1 show that the TRS mutation affects barium block as well as the stability of the conductive state. Comparison of the effects of similar barium resistant mutations in Kcv and Kir2.1 shows that neighboring amino acids in the Kcv selectivity filter affectmore » blocker binding. Conclusions/Significance: The data support the idea that permeant ions have an integral role in stabilizing potassium channel structure, suggest that both barium and amantadine act at a similar site, and demonstrate how genetic selections can be used to map blocker binding sites and reveal mechanistic features.« less

Authors:
 [1];  [2];  [1];  [3];  [1];  [3];  [4];  [5];  [3];  [6]
+ Show Author Affiliations
  1. Univ. of California, San Francisco, CA (United States). Cardiovascular Research Inst.
  2. Univ. degli Studi di Milano (Italy). Dipartimento di Biologia e Istituto di Biofisica del Consiglio Nazionale delle Ricerche
  3. Univ. degli Studi di Milano (Italy). Dipartimento di Biologia e Istituto di Biofisica del Consiglio Nazionale delle Ricerche
  4. Univ. of California, San Francisco, CA (United States). Dept. of Cellular and Molecular Pharmacology
  5. Technical Univ. of Darmstadt (Germany). Inst. fur Botanik
  6. Univ. of California, San Francisco, CA (United States). Dept. of Cellular and Molecular Pharmacology; Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Univ. of California, San Francisco, CA (United States). Dept. of California Inst. for Quantitative Biomedical Research; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Physical Biosciences Division
Publication Date:
Research Org.:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
OSTI Identifier:
1627387
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 4; Journal Issue: 10; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Chatelain, Franck C., Gazzarrini, Sabrina, Fujiwara, Yuichiro, Arrigoni, Cristina, Domigan, Courtney, Ferrara, Giuseppina, Pantoja, Carlos, Thiel, Gerhard, Moroni, Anna, and Minor, Daniel L. Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block. United States: N. p., 2009. Web. doi:10.1371/journal.pone.0007496.
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Chatelain, Franck C., Gazzarrini, Sabrina, Fujiwara, Yuichiro, Arrigoni, Cristina, Domigan, Courtney, Ferrara, Giuseppina, Pantoja, Carlos, Thiel, Gerhard, Moroni, Anna, & Minor, Daniel L. Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block. United States. https://doi.org/10.1371/journal.pone.0007496
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Chatelain, Franck C., Gazzarrini, Sabrina, Fujiwara, Yuichiro, Arrigoni, Cristina, Domigan, Courtney, Ferrara, Giuseppina, Pantoja, Carlos, Thiel, Gerhard, Moroni, Anna, and Minor, Daniel L. Fri . "Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block". United States. https://doi.org/10.1371/journal.pone.0007496. https://www.osti.gov/servlets/purl/1627387.
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@article{osti_1627387,
title = {Selection of Inhibitor-Resistant Viral Potassium Channels Identifies a Selectivity Filter Site that Affects Barium and Amantadine Block},
author = {Chatelain, Franck C. and Gazzarrini, Sabrina and Fujiwara, Yuichiro and Arrigoni, Cristina and Domigan, Courtney and Ferrara, Giuseppina and Pantoja, Carlos and Thiel, Gerhard and Moroni, Anna and Minor, Daniel L.},
abstractNote = {Background: Understanding the interactions between ion channels and blockers remains an important goal that has implications for delineating the basic mechanisms of ion channel function and for the discovery and development of ion channel directed drugs. Methodology/Principal Findings: We used genetic selection methods to probe the interaction of two ion channel blockers, barium and amantadine, with the miniature viral potassium channel Kcv. Selection for Kcv mutants that were resistant to either blocker identified a mutant bearing multiple changes that was resistant to both. Implementation of a PCR shuffling and backcrossing procedure uncovered that the blocker resistance could be attributed to a single change, T63S, at a position that is likely to form the binding site for the inner ion in the selectivity filter (site 4). A combination of electrophysiological and biochemical assays revealed a distinct difference in the ability of the mutant channel to interact with the blockers. Studies of the analogous mutation in the mammalian inward rectifier Kir2.1 show that the TRS mutation affects barium block as well as the stability of the conductive state. Comparison of the effects of similar barium resistant mutations in Kcv and Kir2.1 shows that neighboring amino acids in the Kcv selectivity filter affect blocker binding. Conclusions/Significance: The data support the idea that permeant ions have an integral role in stabilizing potassium channel structure, suggest that both barium and amantadine act at a similar site, and demonstrate how genetic selections can be used to map blocker binding sites and reveal mechanistic features.},
doi = {10.1371/journal.pone.0007496},
journal = {PLoS ONE},
number = 10,
volume = 4,
place = {United States},
year = {Fri Oct 16 00:00:00 EDT 2009},
month = {Fri Oct 16 00:00:00 EDT 2009}
}
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Works referenced in this record:

Screening Blockers Against a Potassium Channel with a Droplet Interface Bilayer Array
journal, November 2008

  • Syeda, Ruhma; Holden, Matthew A.; Hwang, William L.
  • Journal of the American Chemical Society, Vol. 130, Issue 46
  • DOI: 10.1021/ja804968g

A small-molecule screen in C. elegans yields a new calcium channel antagonist
journal, May 2006

  • Kwok, Trevor C. Y.; Ricker, Nicole; Fraser, Regina
  • Nature, Vol. 441, Issue 7089
  • DOI: 10.1038/nature04657

Evolving potassium channels by means of yeast selection reveals structural elements important for selectivity
journal, March 2004

  • Bichet, D.; Lin, Y. -F.; Ibarra, C. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 13
  • DOI: 10.1073/pnas.0401195101

Functional Role and Affinity of Inorganic Cations in Stabilizing the Tetrameric Structure of the KcsA K+ Channel
journal, August 2005

  • Krishnan, Manoj N.; Bingham, Jon-Paul; Lee, Siew Hwee
  • Journal of General Physiology, Vol. 126, Issue 3
  • DOI: 10.1085/jgp.200509323

Tetrameric Stoichiometry of a Prokaryotic K + Channel †
journal, August 1997

  • Heginbotham, Lise; Odessey, Eric; Miller, Christopher
  • Biochemistry, Vol. 36, Issue 33
  • DOI: 10.1021/bi970988i

Multiple transport functions of a red blood cell anion exchanger, tAE1: its role in cell volume regulation
journal, September 2001

Detergent binding explains anomalous SDS-PAGE migration of membrane proteins
journal, January 2009

  • Rath, Arianna; Glibowicka, Mira; Nadeau, Vincent G.
  • Proceedings of the National Academy of Sciences, Vol. 106, Issue 6
  • DOI: 10.1073/pnas.0813167106

A Mutant KcsA K+ Channel with Altered Conduction Properties and Selectivity Filter Ion Distribution
journal, May 2004

Impact of Novel Screening Technologies on Ion Channel Drug Discovery
journal, March 2008

Chlorella virus ATCV-1 encodes a functional potassium channel of 82 amino acids
journal, May 2009

  • Gazzarrini, Sabrina; Kang, Ming; Abenavoli, Alessandra
  • Biochemical Journal, Vol. 420, Issue 2
  • DOI: 10.1042/BJ20090095

A Genetic Screen for Dihydropyridine (DHP)-Resistant Worms Reveals New Residues Required for DHP-Blockage of Mammalian Calcium Channels
journal, May 2008

Ion Channels as Drug Targets: The Next GPCRs
journal, April 2008

  • Kaczorowski, Gregory J.; McManus, Owen B.; Priest, Birgit T.
  • Journal of General Physiology, Vol. 131, Issue 5
  • DOI: 10.1085/jgp.200709946

Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor
journal, April 1995

Structural basis for the function and inhibition of an influenza virus proton channel
journal, January 2008

  • Stouffer, Amanda L.; Acharya, Rudresh; Salom, David
  • Nature, Vol. 451, Issue 7178
  • DOI: 10.1038/nature06528

Ionic Blockage of Sodium Channels in Nerve
journal, June 1973

Determination of Key Structural Requirements of a K+ Channel Pore
journal, January 1997

  • Nakamura, Robert L.; Anderson, Julie A.; Gaber, Richard F.
  • Journal of Biological Chemistry, Vol. 272, Issue 2
  • DOI: 10.1074/jbc.272.2.1011

Thermal Stability of the K + Channel Tetramer: Cation Interactions and the Conserved Threonine Residue at the Innermost Site (S 4 ) of the KcsA Selectivity Filter
journal, April 2008

  • Krishnan, Manoj N.; Trombley, Patrick; Moczydlowski, Edward G.
  • Biochemistry, Vol. 47, Issue 19
  • DOI: 10.1021/bi702281p

Molecular Properties of Kcv, a Virus Encoded K + Channel
journal, January 2007

  • Pagliuca, Cinzia; Goetze, Tom Alexander; Wagner, Richard
  • Biochemistry, Vol. 46, Issue 4
  • DOI: 10.1021/bi061530w

The S4 Voltage Sensor Packs Against the Pore Domain in the KAT1 Voltage-Gated Potassium Channel
journal, August 2005

Novel Neuroprotective K + Channel Inhibitor Identified by High-Throughput Screening in Yeast
journal, January 2004

  • Zaks-Makhina, Elena; Kim, Yonjung; Aizenman, Elias
  • Molecular Pharmacology, Vol. 65, Issue 1
  • DOI: 10.1124/mol.65.1.214

Crystallographic Study of the Tetrabutylammonium Block to the KcsA K+ Channel
journal, February 2007

Functional expression of a vertebrate inwardly rectifying K+ channel in yeast.
journal, September 1995

  • Tang, W.; Ruknudin, A.; Yang, W. P.
  • Molecular Biology of the Cell, Vol. 6, Issue 9
  • DOI: 10.1091/mbc.6.9.1231

Rapid evolution of a protein in vitro by DNA shuffling
journal, August 1994

Amantadine and sparteine inhibit ATP-regulated K-currents in the insulin-secreting β-cell line, HIT-T15
journal, November 1991

Structure and mechanism of the M2 proton channel of influenza A virus
journal, January 2008

hERG potassium channels and cardiac arrhythmia
journal, March 2006

  • Sanguinetti, Michael C.; Tristani-Firouzi, Martin
  • Nature, Vol. 440, Issue 7083
  • DOI: 10.1038/nature04710

Partitioning and Localization of Spin-Labeled Amantadine in Lipid Bilayers: An Epr Study
journal, October 1998

  • Subczynski, Witold K.; Wojas, Jerzy; Pezeshk, Vida
  • Journal of Pharmaceutical Sciences, Vol. 87, Issue 10
  • DOI: 10.1021/js970381n

Transmembrane Structure of an Inwardly Rectifying Potassium Channel
journal, March 1999

In vitro synthesis, tetramerization and single channel characterization of virus-encoded potassium channel Kcv
journal, February 2007

Ion Conduction and Selectivity in K + Channels
journal, June 2005

Chemistry of ion coordination and hydration revealed by a K+ channel–Fab complex at 2.0 Å resolution
journal, November 2001

  • Zhou, Yufeng; Morais-Cabral, João H.; Kaufman, Amelia
  • Nature, Vol. 414, Issue 6859
  • DOI: 10.1038/35102009

Integrating the discovery pipeline for novel compounds targeting ion channels
journal, August 2008

Small potassium ion channel proteins encoded by chlorella viruses
journal, February 2004

  • Kang, M.; Moroni, A.; Gazzarrini, S.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 15
  • DOI: 10.1073/pnas.0307824100

The Pore Helix Dipole Has a Minor Role in Inward Rectifier Channel Function
journal, September 2005

Predicting drug–hERG channel interactions that cause acquired long QT syndrome
journal, March 2005

  • Sanguinetti, Michael C.; Mitcheson, John S.
  • Trends in Pharmacological Sciences, Vol. 26, Issue 3
  • DOI: 10.1016/j.tips.2005.01.003

Structure prediction for the down state of a potassium channel voltage sensor
journal, December 2006

  • Grabe, Michael; Lai, Helen C.; Jain, Monika
  • Nature, Vol. 445, Issue 7127
  • DOI: 10.1038/nature05494

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.
journal, October 1994

Amantadine partition and localization in phospholipid membrane: a solution NMR study
journal, November 2004

  • Wang, Junfeng; Schnell, Jason R.; Chou, James J.
  • Biochemical and Biophysical Research Communications, Vol. 324, Issue 1
  • DOI: 10.1016/j.bbrc.2004.09.039

High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans
journal, November 2006

  • Burns, Andrew R.; Kwok, Trevor C. Y.; Howard, Al
  • Nature Protocols, Vol. 1, Issue 4
  • DOI: 10.1038/nprot.2006.283

High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology
journal, April 2008

  • Dunlop, John; Bowlby, Mark; Peri, Ravikumar
  • Nature Reviews Drug Discovery, Vol. 7, Issue 4
  • DOI: 10.1038/nrd2552

Structural Dynamics of the Streptomyces lividans K + Channel (SKC1):  Oligomeric Stoichiometry and Stability † , ‡
journal, August 1997

  • Cortes, D. Marien; Perozo, Eduardo
  • Biochemistry, Vol. 36, Issue 33
  • DOI: 10.1021/bi971018y

Structural and Thermodynamic Properties of Selective Ion Binding in a K+ Channel
journal, May 2007

Transmembrane domain length of viral K+ channels is a signal for mitochondria targeting
journal, August 2008

  • Balss, J.; Papatheodorou, P.; Mehmel, M.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 34
  • DOI: 10.1073/pnas.0805709105

Structural basis of TEA blockade in a model potassium channel
journal, April 2005

  • Lenaeus, Michael J.; Vamvouka, Magdalini; Focia, Pamela J.
  • Nature Structural & Molecular Biology, Vol. 12, Issue 5
  • DOI: 10.1038/nsmb929

A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1
journal, March 2000

The Art of Dissecting the Function of a Potassium Channel
journal, September 2005

Fast and slow gating are inherent properties of the pore module of the K+ channel Kcv
journal, August 2009

  • Abenavoli, Alessandra; DiFrancesco, Mattia Lorenzo; Schroeder, Indra
  • Journal of General Physiology, Vol. 134, Issue 3
  • DOI: 10.1085/jgp.200910266

The Barium Site in a Potassium Channel by X-Ray Crystallography: Figure 1
journal, February 2000

  • Jiang, Youxing; MacKinnon, Roderick
  • The Journal of General Physiology, Vol. 115, Issue 3
  • DOI: 10.1085/jgp.115.3.269

A novel potassium channel encoded by Ectocarpus siliculosus virus
journal, January 2005

  • Chen, Jun; Cassar, Steven C.; Zhang, Di
  • Biochemical and Biophysical Research Communications, Vol. 326, Issue 4
  • DOI: 10.1016/j.bbrc.2004.11.125

Crystal Structure of a Mammalian Voltage-Dependent Shaker Family K+ Channel
journal, August 2005

Refolding of an integral membrane protein. OmpA of Escherichia coli.
journal, November 1990

The location of amantadine hydrochloride and free base within phospholipid multilayers: a neutron and X-ray diffraction study
journal, January 1993

  • Duff, K. C.; Cudmore, A. J.; Bradshaw, J. P.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1145, Issue 1
  • DOI: 10.1016/0005-2736(93)90392-d

Amantadine partition and localization in phospholipid membrane: a solution NMR study
journal, November 2004

  • Wang, Junfeng; Schnell, Jason R.; Chou, James J.
  • Biochemical and Biophysical Research Communications, Vol. 324, Issue 1
  • DOI: 10.1016/j.bbrc.2004.09.039

A novel potassium channel encoded by Ectocarpus siliculosus virus
journal, January 2005

  • Chen, Jun; Cassar, Steven C.; Zhang, Di
  • Biochemical and Biophysical Research Communications, Vol. 326, Issue 4
  • DOI: 10.1016/j.bbrc.2004.11.125

Integrating the discovery pipeline for novel compounds targeting ion channels
journal, August 2008

In vitro synthesis, tetramerization and single channel characterization of virus-encoded potassium channel Kcv
journal, February 2007

Crystallographic Study of the Tetrabutylammonium Block to the KcsA K+ Channel
journal, February 2007

The S4 Voltage Sensor Packs Against the Pore Domain in the KAT1 Voltage-Gated Potassium Channel
journal, August 2005

The Pore Helix Dipole Has a Minor Role in Inward Rectifier Channel Function
journal, September 2005

The Art of Dissecting the Function of a Potassium Channel
journal, September 2005

Predicting drug–hERG channel interactions that cause acquired long QT syndrome
journal, March 2005

  • Sanguinetti, Michael C.; Mitcheson, John S.
  • Trends in Pharmacological Sciences, Vol. 26, Issue 3
  • DOI: 10.1016/j.tips.2005.01.003

The Pore Helix Is Involved in Stabilizing the Open State of Inwardly Rectifying K+ Channels
journal, July 2003

Data transformations for improved display and fitting of single-channel dwell time histograms
journal, December 1987

Mutations in the K+ channel signature sequence
journal, April 1994

Yeast Screen for Constitutively Active Mutant G Protein–Activated Potassium Channels
journal, March 2001

Coupling Gβγ-Dependent Activation to Channel Opening via Pore Elements in Inwardly Rectifying Potassium Channels
journal, March 2001

Molecular Properties of Kcv, a Virus Encoded K + Channel
journal, January 2007

  • Pagliuca, Cinzia; Goetze, Tom Alexander; Wagner, Richard
  • Biochemistry, Vol. 46, Issue 4
  • DOI: 10.1021/bi061530w

Tetrameric Stoichiometry of a Prokaryotic K + Channel †
journal, August 1997

  • Heginbotham, Lise; Odessey, Eric; Miller, Christopher
  • Biochemistry, Vol. 36, Issue 33
  • DOI: 10.1021/bi970988i

Structural Dynamics of the Streptomyces lividans K + Channel (SKC1):  Oligomeric Stoichiometry and Stability † , ‡
journal, August 1997

  • Cortes, D. Marien; Perozo, Eduardo
  • Biochemistry, Vol. 36, Issue 33
  • DOI: 10.1021/bi971018y

Screening Blockers Against a Potassium Channel with a Droplet Interface Bilayer Array
journal, November 2008

  • Syeda, Ruhma; Holden, Matthew A.; Hwang, William L.
  • Journal of the American Chemical Society, Vol. 130, Issue 46
  • DOI: 10.1021/ja804968g

Partitioning and Localization of Spin-Labeled Amantadine in Lipid Bilayers: An Epr Study
journal, October 1998

  • Subczynski, Witold K.; Wojas, Jerzy; Pezeshk, Vida
  • Journal of Pharmaceutical Sciences, Vol. 87, Issue 10
  • DOI: 10.1021/js970381n

Rapid evolution of a protein in vitro by DNA shuffling
journal, August 1994

A small-molecule screen in C. elegans yields a new calcium channel antagonist
journal, May 2006

  • Kwok, Trevor C. Y.; Ricker, Nicole; Fraser, Regina
  • Nature, Vol. 441, Issue 7089
  • DOI: 10.1038/nature04657

Structure prediction for the down state of a potassium channel voltage sensor
journal, December 2006

  • Grabe, Michael; Lai, Helen C.; Jain, Monika
  • Nature, Vol. 445, Issue 7127
  • DOI: 10.1038/nature05494

Structure and mechanism of the M2 proton channel of influenza A virus
journal, January 2008

High-throughput screening of small molecules for bioactivity and target identification in Caenorhabditis elegans
journal, November 2006

  • Burns, Andrew R.; Kwok, Trevor C. Y.; Howard, Al
  • Nature Protocols, Vol. 1, Issue 4
  • DOI: 10.1038/nprot.2006.283

High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology
journal, April 2008

  • Dunlop, John; Bowlby, Mark; Peri, Ravikumar
  • Nature Reviews Drug Discovery, Vol. 7, Issue 4
  • DOI: 10.1038/nrd2552

Structural basis of TEA blockade in a model potassium channel
journal, April 2005

  • Lenaeus, Michael J.; Vamvouka, Magdalini; Focia, Pamela J.
  • Nature Structural & Molecular Biology, Vol. 12, Issue 5
  • DOI: 10.1038/nsmb929

Controlling influenza virus replication by inhibiting its proton channel
journal, January 2007

  • Pinto, Lawrence H.; Lamb, Robert A.
  • Mol. BioSyst., Vol. 3, Issue 1
  • DOI: 10.1039/b611613m

Small potassium ion channel proteins encoded by chlorella viruses
journal, February 2004

  • Kang, M.; Moroni, A.; Gazzarrini, S.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 15
  • DOI: 10.1073/pnas.0307824100

Evolving potassium channels by means of yeast selection reveals structural elements important for selectivity
journal, March 2004

  • Bichet, D.; Lin, Y. -F.; Ibarra, C. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 13
  • DOI: 10.1073/pnas.0401195101

Transmembrane domain length of viral K+ channels is a signal for mitochondria targeting
journal, August 2008

  • Balss, J.; Papatheodorou, P.; Mehmel, M.
  • Proceedings of the National Academy of Sciences, Vol. 105, Issue 34
  • DOI: 10.1073/pnas.0805709105

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution.
journal, October 1994

Determination of Key Structural Requirements of a K+ Channel Pore
journal, January 1997

  • Nakamura, Robert L.; Anderson, Julie A.; Gaber, Richard F.
  • Journal of Biological Chemistry, Vol. 272, Issue 2
  • DOI: 10.1074/jbc.272.2.1011

Differential Roles of Blocking Ions in KirBac1.1 Tetramer Stability
journal, January 2009

  • Wang, Shizhen; Alimi, Yewande; Tong, Ailing
  • Journal of Biological Chemistry, Vol. 284, Issue 5
  • DOI: 10.1074/jbc.m807474200

Specific and Slow Inhibition of the Kir2.1 K+ Channel by Gambogic Acid
journal, June 2009

  • Zaks-Makhina, Elena; Li, Hui; Grishin, Anatoly
  • Journal of Biological Chemistry, Vol. 284, Issue 23
  • DOI: 10.1074/jbc.m901586200

Functional Role and Affinity of Inorganic Cations in Stabilizing the Tetrameric Structure of the KcsA K+ Channel
journal, August 2005

  • Krishnan, Manoj N.; Bingham, Jon-Paul; Lee, Siew Hwee
  • Journal of General Physiology, Vol. 126, Issue 3
  • DOI: 10.1085/jgp.200509323

Fast and slow gating are inherent properties of the pore module of the K+ channel Kcv
journal, August 2009

  • Abenavoli, Alessandra; DiFrancesco, Mattia Lorenzo; Schroeder, Indra
  • Journal of General Physiology, Vol. 134, Issue 3
  • DOI: 10.1085/jgp.200910266

Ionic Blockage of Sodium Channels in Nerve
journal, June 1973

Functional expression of a vertebrate inwardly rectifying K+ channel in yeast.
journal, September 1995

  • Tang, W.; Ruknudin, A.; Yang, W. P.
  • Molecular Biology of the Cell, Vol. 6, Issue 9
  • DOI: 10.1091/mbc.6.9.1231

Amantadine and sparteine inhibit ATP-regulated K-currents in the insulin-secreting β-cell line, HIT-T15
journal, November 1991

Novel Neuroprotective K + Channel Inhibitor Identified by High-Throughput Screening in Yeast
journal, January 2004

  • Zaks-Makhina, Elena; Kim, Yonjung; Aizenman, Elias
  • Molecular Pharmacology, Vol. 65, Issue 1
  • DOI: 10.1124/mol.65.1.214

Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor
journal, April 1995

Ion Conduction and Selectivity in K + Channels
journal, June 2005

Structural and Thermodynamic Properties of Selective Ion Binding in a K+ Channel
journal, May 2007

Impact of Novel Screening Technologies on Ion Channel Drug Discovery
journal, March 2008

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    journal, September 2010

    • Paynter, Jennifer J.; Shang, Lijun; Bollepalli, Murali K.
    • Channels, Vol. 4, Issue 5
    • DOI: 10.4161/chan.4.5.13006

    Engineering of a light-gated potassium channel
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    • DOI: 10.5167/uzh-186964

    Role of Methyl-Induced Polarization in Ion Binding
    journal, January 2014

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    • DOI: 10.1007/s00232-018-0022-2

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    journal, February 2018

    • Andersson, Alma E. V.; Kasimova, Marina A.; Delemotte, Lucie
    • The Journal of Membrane Biology, Vol. 251, Issue 3
    • DOI: 10.1007/s00232-018-0022-2

    Role of Methyl-Induced Polarization in Ion Binding
    journal, January 2014

    A High-Throughput Functional Screen Identifies Small Molecule Regulators of Temperature- and Mechano-Sensitive K 2P Channels
    journal, June 2013

    • Bagriantsev, Sviatoslav N.; Ang, Kean-Hooi; Gallardo-Godoy, Alejandra
    • ACS Chemical Biology, Vol. 8, Issue 8
    • DOI: 10.1021/cb400289x

    Tethered Protein Display Identifies a Novel Kir3.2 (GIRK2) Regulator from Protein Scaffold Libraries
    journal, July 2014

    • Bagriantsev, Sviatoslav N.; Chatelain, Franck C.; Clark, Kimberly A.
    • ACS Chemical Neuroscience, Vol. 5, Issue 9
    • DOI: 10.1021/cn5000698

    The voltage-sensing domain of a phosphatase gates the pore of a potassium channel
    journal, February 2013

    • Arrigoni, Cristina; Schroeder, Indra; Romani, Giulia
    • Journal of General Physiology, Vol. 141, Issue 3
    • DOI: 10.1085/jgp.201210940

    Identification of Putative Potassium Channel Homologues in Pathogenic Protozoa
    journal, February 2012

    Engineering a Ca++-Sensitive (Bio)Sensor from the Pore-Module of a Potassium Channel
    journal, February 2015

    • DiFrancesco, Mattia; Gazzarrini, Sabrina; Arrigoni, Cristina
    • Sensors, Vol. 15, Issue 3
    • DOI: 10.3390/s150304913

    Genes for Membrane Transport Proteins: Not So Rare in Viruses
    journal, August 2018

    • Greiner, Timo; Moroni, Anna; Van Etten, James
    • Viruses, Vol. 10, Issue 9
    • DOI: 10.3390/v10090456

    A small viral potassium ion channel with an inherent inward rectification
    text, January 2019

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