An insight into statistical refractive index properties of cell internal structure via low-coherence statistical amplitude microscopy

Journal Article · 29 September 2010 · Optics Express

DOI: https://doi.org/10.1364/oe.18.021950 · OSTI ID:1627124

Wang, Pin ^[1]; Bista, Rajan K. ^[2]; Qiu, Wei ^[2]; Khalbuss, Walid E. ^[2]; Zhang, Lin ^[2]; Brand, Randall E. ^[2]; Liu, Yang ^[2]

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1. University of Pittsburgh, PA (United States); DOE/OSTI
2. University of Pittsburgh, PA (United States)

Refractive index properties, especially at the nanoscale, have shown great potential in cancer diagnosis and screening. Due to the intrinsic complexity and weak refractive index fluctuation, the reconstruction of internal structures of a biological cell has been challenging. In this paper, we propose a simple and practical approach to derive the statistical properties of internal refractive index fluctuations within a biological cell with a new optical microscopy method – Low-coherence Statistical Amplitude Microscopy (SAM). We validated the capability of SAM to characterize the statistical properties of cell internal structures, which is described by numerical models of one-dimensional Gaussian random field. We demonstrated the potential of SAM in cancer detection with an animal model of intestinal carcinogenesis – multiple intestinal neoplasia mouse model. We showed that SAM-derived statistical properties of cell nuclear structures could detect the subtle changes that are otherwise undetectable by conventional cytopathology.

• Research Organization: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)
• Sponsoring Organization: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER); University of Pittsburgh Medical Center
• Grant/Contract Number: SC0014664
• OSTI ID: 1627124
• Journal Information: Optics Express, Journal Name: Optics Express Journal Issue: 21 Vol. 18; ISSN 1094-4087; ISSN OPEXFF
• Publisher: Optical Society of America (OSA)Copyright Statement
• Country of Publication: United States
• Language: English