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Title: Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production

Journal Article · · Biotechnology for Biofuels
ORCiD logo [1];  [1];  [2];  [3];  [2];  [1];  [1];  [3];  [1]
  1. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation
  2. Dartmouth College, Hanover, NH (United States). Thayer School of Engineering
  3. Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation

Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been variably successful. Here, we aim to understand this variation by a multifaceted approach including genomic and transcriptomic analysis combined with chemostat cultivation and high solids cellulose fermentation. Three strain lineages comprising 16 strains total were examined. Two strain lineages in which genes involved in pathways leading to organic acids and/or sporulation had been knocked out resulted in four end-strains after adaptive laboratory evolution (ALE). A third strain lineage recapitulated mutations involving adhE that occurred spontaneously in some of the engineered strains.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1627007
Journal Information:
Biotechnology for Biofuels, Vol. 13, Issue 1; ISSN 1754-6834
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways journal May 2013
The identification of four histidine kinases that influence sporulation in Clostridium thermocellum journal August 2014
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405) journal January 2012
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Ethanol production by engineered thermophiles journal June 2015
Co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum for enhancing hydrogen production via thermophilic fermentation of cornstalk waste journal July 2012
Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs) journal December 2016
Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans journal November 2017
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Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum journal January 2013
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Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield journal September 2008
Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum journal August 2011
Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii journal June 2014
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Figures / Tables (9)