Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production
Abstract
Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been variably successful. Here, we aim to understand this variation by a multifaceted approach including genomic and transcriptomic analysis combined with chemostat cultivation and high solids cellulose fermentation. Three strain lineages comprising 16 strains total were examined. Two strain lineages in which genes involved in pathways leading to organic acids and/or sporulation had been knocked out resulted in four end-strains after adaptive laboratory evolution (ALE). A third strain lineage recapitulated mutations involving adhE that occurred spontaneously in some of the engineered strains.
- Authors:
-
[1];
- Dartmouth College, Hanover, NH (United States). Thayer School of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation
- Dartmouth College, Hanover, NH (United States). Thayer School of Engineering
- Univ. of Wisconsin, Madison, WI (United States). Dept. of Bacteriology; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1627007
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 13; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Biotechnology & Applied Microbiology; Energy & Fuels
Citation Formats
Holwerda, Evert K., Olson, Daniel G., Ruppertsberger, Natalie M., Stevenson, David M., Murphy, Sean J. L., Maloney, Marybeth I., Lanahan, Anthony A., Amador-Noguez, Daniel, and Lynd, Lee R.. Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production. United States: N. p., 2020.
Web. doi:10.1186/s13068-020-01680-5.
Holwerda, Evert K., Olson, Daniel G., Ruppertsberger, Natalie M., Stevenson, David M., Murphy, Sean J. L., Maloney, Marybeth I., Lanahan, Anthony A., Amador-Noguez, Daniel, & Lynd, Lee R.. Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production. United States. https://doi.org/10.1186/s13068-020-01680-5
Holwerda, Evert K., Olson, Daniel G., Ruppertsberger, Natalie M., Stevenson, David M., Murphy, Sean J. L., Maloney, Marybeth I., Lanahan, Anthony A., Amador-Noguez, Daniel, and Lynd, Lee R.. Tue .
"Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production". United States. https://doi.org/10.1186/s13068-020-01680-5. https://www.osti.gov/servlets/purl/1627007.
@article{osti_1627007,
title = {Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production},
author = {Holwerda, Evert K. and Olson, Daniel G. and Ruppertsberger, Natalie M. and Stevenson, David M. and Murphy, Sean J. L. and Maloney, Marybeth I. and Lanahan, Anthony A. and Amador-Noguez, Daniel and Lynd, Lee R.},
abstractNote = {Engineering efforts targeted at increasing ethanol by modifying the central fermentative metabolism of Clostridium thermocellum have been variably successful. Here, we aim to understand this variation by a multifaceted approach including genomic and transcriptomic analysis combined with chemostat cultivation and high solids cellulose fermentation. Three strain lineages comprising 16 strains total were examined. Two strain lineages in which genes involved in pathways leading to organic acids and/or sporulation had been knocked out resulted in four end-strains after adaptive laboratory evolution (ALE). A third strain lineage recapitulated mutations involving adhE that occurred spontaneously in some of the engineered strains.},
doi = {10.1186/s13068-020-01680-5},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 13,
place = {United States},
year = {2020},
month = {3}
}
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Fig. 1: Overview of the strain lineages and data associated with each strain (a) and overview of the research approach and data analysis in this paper (b). A total of 16 strains were analyzed, covering three different lineages. For each stage of the analysis different data sets were collected. Fourmore »
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Works referenced in this record:
Engineering Cellular Metabolism
journal, March 2016
- Nielsen, Jens; Keasling, Jay D.
- Cell, Vol. 164, Issue 6
Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction
journal, January 2013
- Resch, Michael G.; Donohoe, Bryon S.; Baker, John O.
- Energy & Environmental Science, Vol. 6, Issue 6
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment
journal, January 2016
- Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass
journal, January 2019
- Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad
- Biotechnology for Biofuels, Vol. 12, Issue 1
Ethanol production by engineered thermophiles
journal, June 2015
- Olson, Daniel G.; Sparling, Richard; Lynd, Lee R.
- Current Opinion in Biotechnology, Vol. 33
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008
- Shaw, A. J.; Podkaminer, K. K.; Desai, S. G.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774
Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways
journal, May 2013
- van der Veen, Douwe; Lo, Jonathan; Brown, Steven D.
- Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 7
The identification of four histidine kinases that influence sporulation in Clostridium thermocellum
journal, August 2014
- Mearls, Elizabeth B.; Lynd, Lee R.
- Anaerobe, Vol. 28
The Bifunctional Alcohol and Aldehyde Dehydrogenase Gene, adhE , Is Necessary for Ethanol Production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum
journal, February 2015
- Lo, Jonathan; Zheng, Tianyong; Hon, Shuen
- Journal of Bacteriology, Vol. 197, Issue 8
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405)
journal, January 2012
- Ellis, Lucas D.; Holwerda, Evert K.; Hogsett, David
- Bioresource Technology, Vol. 103, Issue 1
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
journal, October 2014
- Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations : Overflow Metabolism and Growth Cessation in
journal, August 2017
- Thompson, R. Adam; Trinh, Cong T.
- Biotechnology and Bioengineering, Vol. 114, Issue 11
The ethanol pathway from Thermoanaerobacterium saccharolyticum improves ethanol production in Clostridium thermocellum
journal, July 2017
- Hon, Shuen; Olson, Daniel G.; Holwerda, Evert K.
- Metabolic Engineering, Vol. 42
End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405
journal, August 2011
- Rydzak, Thomas; Levin, David B.; Cicek, Nazim
- Applied Microbiology and Biotechnology, Vol. 92, Issue 1
Co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum for enhancing hydrogen production via thermophilic fermentation of cornstalk waste
journal, July 2012
- Li, Qian; Liu, Chun-Zhao
- International Journal of Hydrogen Energy, Vol. 37, Issue 14
Metabolic selectivity and growth of Clostridium thermocellum in continuous culture under elevated hydrostatic pressure
journal, January 2004
- Bothun, G. D.; Knutson, B. L.; Berberich, J. A.
- Applied Microbiology and Biotechnology, Vol. 65, Issue 2
Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum
journal, January 2015
- Biswas, Ranjita; Zheng, Tianyong; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum
journal, August 2011
- Brown, S. D.; Guss, A. M.; Karpinets, T. V.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 33
Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum
journal, August 2011
- Shao, Xiongjun; Raman, Babu; Zhu, Mingjun
- Applied Microbiology and Biotechnology, Vol. 92, Issue 3
Mutator action by Escherichia coli strains carrying dnaE mutations
journal, September 1977
- Sevastopoulos, C. G.; Glaser, D. A.
- Proceedings of the National Academy of Sciences, Vol. 74, Issue 9
Sequence analysis and phenotypes of five temperature sensitive mutator alleles of dnaE, encoding modified α-catalytic subunits of Escherichia coli DNA polymerase III holoenzyme
journal, January 2002
- Vandewiele, Dominique; Fernández de Henestrosa, Antonio R.; Timms, Andrew R.
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 499, Issue 1
Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria
journal, November 2017
- Eminoğlu, Ayşenur; Murphy, Sean Jean-Loup; Maloney, Marybeth
- Biotechnology for Biofuels, Vol. 10, Issue 1
Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs)
journal, December 2016
- Hon, Shuen; Lanahan, Anthony A.; Tian, Liang
- Metabolic Engineering Communications, Vol. 3
Anaerobic detoxification of acetic acid in a thermophilic ethanologen
journal, May 2015
- Shaw, A. Joe; Miller, Bethany B.; Rogers, Stephen R.
- Biotechnology for Biofuels, Vol. 8, Issue 1
A defined growth medium with very low background carbon for culturing Clostridium thermocellum
journal, February 2012
- Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R.
- Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6
Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum
journal, November 2015
- Papanek, Beth; Biswas, Ranjita; Rydzak, Thomas
- Metabolic Engineering, Vol. 32
Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
journal, June 2016
- Tian, Liang; Papanek, Beth; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013
- Deng, Yu; Olson, Daniel G.; Zhou, Jilai
- Metabolic Engineering, Vol. 15, p. 151-158
Stability of Glutamine and Pyroglutamic Acid under Model System Conditions: Influence of Physical and Technological Factors
journal, November 1987
- Airaudo, Charles B.; Gayte-Sorbier, AndrÉ; Armand, Paul
- Journal of Food Science, Vol. 52, Issue 6
Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellum
journal, May 2017
- Rydzak, Thomas; Garcia, David; Stevenson, David M.
- Metabolic Engineering, Vol. 41
Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum
journal, September 2015
- Lin, Paul P.; Mi, Luo; Morioka, Amy H.
- Metabolic Engineering, Vol. 31
Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production
journal, September 2018
- Hon, Shuen; Holwerda, Evert K.; Worthen, Robert S.
- Biotechnology for Biofuels, Vol. 11, Issue 1
Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol yield from cellulose and switchgrass fermentations
journal, January 2012
- Li, Yongchao; Tschaplinski, Timothy J.; Engle, Nancy L.
- Biotechnology for Biofuels, Vol. 5, Issue 1
Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
journal, June 2014
- Chung, D.; Cha, M.; Guss, A. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose
journal, March 2011
- Higashide, Wendy; Li, Yongchao; Yang, Yunfeng
- Applied and Environmental Microbiology, Vol. 77, Issue 8
Rational improvement of the engineered isobutanol-producing Bacillus subtilis by elementary mode analysis
journal, January 2012
- Li, Shanshan; Huang, Di; Li, Yong
- Microbial Cell Factories, Vol. 11, Issue 1
Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose
journal, January 2016
- Gaida, Stefan Marcus; Liedtke, Andrea; Jentges, Andreas Heinz Wilhelm
- Microbial Cell Factories, Vol. 15, Issue 1
Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose
journal, November 2015
- Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian
- Metabolic Engineering, Vol. 32
Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans
journal, November 2017
- Ou, Jianfa; Xu, Ningning; Ernst, Patrick
- Process Biochemistry, Vol. 62
Improved n -Butanol Production from Clostridium cellulovorans by Integrated Metabolic and Evolutionary Engineering
journal, January 2019
- Wen, Zhiqiang; Ledesma-Amaro, Rodrigo; Lin, Jianping
- Applied and Environmental Microbiology, Vol. 85, Issue 7
n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases
journal, August 2019
- Bao, Teng; Zhao, Jingbo; Li, Jing
- Bioresource Technology, Vol. 285
Production of amino acids – Genetic and metabolic engineering approaches
journal, December 2017
- Lee, Jin-Ho; Wendisch, Volker F.
- Bioresource Technology, Vol. 245
From zero to hero—Design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production
journal, March 2011
- Becker, Judith; Zelder, Oskar; Häfner, Stefan
- Metabolic Engineering, Vol. 13, Issue 2
Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum
journal, November 2015
- Mahr, Regina; Gätgens, Cornelia; Gätgens, Jochem
- Metabolic Engineering, Vol. 32
Assembly of a novel biosynthetic pathway for gentamicin B production in Micromonospora echinospora
journal, January 2016
- Ni, Xianpu; Sun, Zhenpeng; Gu, Yawen
- Microbial Cell Factories, Vol. 15, Issue 1
Miniaturized and automated adaptive laboratory evolution: Evolving Corynebacterium glutamicum towards an improved d-xylose utilization
journal, December 2017
- Radek, Andreas; Tenhaef, Niklas; Müller, Moritz Fabian
- Bioresource Technology, Vol. 245
Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
journal, January 2017
- Zhu, Duolong; Fu, Yuxin; Liu, Fulu
- Microbial Cell Factories, Vol. 16, Issue 1
Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate
journal, April 2019
- Wen, Jingbai; Bao, Jie
- Biotechnology for Biofuels, Vol. 12, Issue 1
Bio-based production of chemicals, materials and fuels – Corynebacterium glutamicum as versatile cell factory
journal, August 2012
- Becker, Judith; Wittmann, Christoph
- Current Opinion in Biotechnology, Vol. 23, Issue 4
Corynebacterium glutamicum as platform for the production of hydroxybenzoic acids
journal, May 2018
- Kallscheuer, Nicolai; Marienhagen, Jan
- Microbial Cell Factories, Vol. 17, Issue 1
Recent progress in consolidated bioprocessing
journal, June 2012
- Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
- Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
journal, June 2016
- Herring, Christopher D.; Kenealy, William R.; Joe Shaw, A.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Nicotinamide cofactor ratios in engineered strains of Clostridium thermocellum and Thermoanaerobacterium saccharolyticum
journal, April 2016
- Beri, Dhananjay; Olson, Daniel G.; Holwerda, Evert K.
- FEMS Microbiology Letters, Vol. 363, Issue 11
Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms
journal, April 2013
- Holwerda, Evert K.; Ellis, Lucas D.; Lynd, Lee R.
- Biotechnology and Bioengineering, Vol. 110, Issue 9
Development of a genome-editing CRISPR/Cas9 system in thermophilic fungal Myceliophthora species and its application to hyper-cellulase production strain engineering
journal, January 2017
- Liu, Qian; Gao, Ranran; Li, Jingen
- Biotechnology for Biofuels, Vol. 10, Issue 1
Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum
journal, April 2013
- Holwerda, Evert K.; Lynd, Lee R.
- Biotechnology and Bioengineering, Vol. 110, Issue 9
Cross-feeding and wheat straw extractives enhance growth of Clostridium thermocellum-containing co-cultures for consolidated bioprocessing
journal, January 2021
- Froese, Alan G.; Sparling, Richard
- Bioprocess and Biosystems Engineering, Vol. 44, Issue 4
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose
journal, September 2012
- Higashide, Wendy; Li, Yongchao; Yang, Yunfeng
- Applied and Environmental Microbiology, Vol. 78, Issue 19
Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum
journal, April 2013
- Holwerda, Evert K.; Lynd, Lee R.
- Biotechnology and Bioengineering, Vol. 110, Issue 9
Overflow metabolism and growth cessation in Clostridium thermocellum DSM1313 during high cellulose loading fermentations : Overflow Metabolism and Growth Cessation in
journal, August 2017
- Thompson, R. Adam; Trinh, Cong T.
- Biotechnology and Bioengineering, Vol. 114, Issue 11
Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum
journal, August 2011
- Shao, Xiongjun; Raman, Babu; Zhu, Mingjun
- Applied Microbiology and Biotechnology, Vol. 92, Issue 3
End-product induced metabolic shifts in Clostridium thermocellum ATCC 27405
journal, August 2011
- Rydzak, Thomas; Levin, David B.; Cicek, Nazim
- Applied Microbiology and Biotechnology, Vol. 92, Issue 1
A defined growth medium with very low background carbon for culturing Clostridium thermocellum
journal, February 2012
- Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R.
- Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6
Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways
journal, May 2013
- van der Veen, Douwe; Lo, Jonathan; Brown, Steven D.
- Journal of Industrial Microbiology & Biotechnology, Vol. 40, Issue 7
The identification of four histidine kinases that influence sporulation in Clostridium thermocellum
journal, August 2014
- Mearls, Elizabeth B.; Lynd, Lee R.
- Anaerobe, Vol. 28
Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405)
journal, January 2012
- Ellis, Lucas D.; Holwerda, Evert K.; Hogsett, David
- Bioresource Technology, Vol. 103, Issue 1
Production of amino acids – Genetic and metabolic engineering approaches
journal, December 2017
- Lee, Jin-Ho; Wendisch, Volker F.
- Bioresource Technology, Vol. 245
n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases
journal, August 2019
- Bao, Teng; Zhao, Jingbo; Li, Jing
- Bioresource Technology, Vol. 285
Engineering Cellular Metabolism
journal, March 2016
- Nielsen, Jens; Keasling, Jay D.
- Cell, Vol. 164, Issue 6
Bio-based production of chemicals, materials and fuels – Corynebacterium glutamicum as versatile cell factory
journal, August 2012
- Becker, Judith; Wittmann, Christoph
- Current Opinion in Biotechnology, Vol. 23, Issue 4
Ethanol production by engineered thermophiles
journal, June 2015
- Olson, Daniel G.; Sparling, Richard; Lynd, Lee R.
- Current Opinion in Biotechnology, Vol. 33
Co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum for enhancing hydrogen production via thermophilic fermentation of cornstalk waste
journal, July 2012
- Li, Qian; Liu, Chun-Zhao
- International Journal of Hydrogen Energy, Vol. 37, Issue 14
Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs)
journal, December 2016
- Hon, Shuen; Lanahan, Anthony A.; Tian, Liang
- Metabolic Engineering Communications, Vol. 3
Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans
journal, November 2017
- Ou, Jianfa; Xu, Ningning; Ernst, Patrick
- Process Biochemistry, Vol. 62
From zero to hero—Design-based systems metabolic engineering of Corynebacterium glutamicum for l-lysine production
journal, March 2011
- Becker, Judith; Zelder, Oskar; Häfner, Stefan
- Metabolic Engineering, Vol. 13, Issue 2
Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013
- Deng, Yu; Olson, Daniel G.; Zhou, Jilai
- Metabolic Engineering, Vol. 15, p. 151-158
Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum
journal, September 2015
- Lin, Paul P.; Mi, Luo; Morioka, Amy H.
- Metabolic Engineering, Vol. 31
Metabolic and process engineering of Clostridium cellulovorans for biofuel production from cellulose
journal, November 2015
- Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian
- Metabolic Engineering, Vol. 32
Elimination of metabolic pathways to all traditional fermentation products increases ethanol yields in Clostridium thermocellum
journal, November 2015
- Papanek, Beth; Biswas, Ranjita; Rydzak, Thomas
- Metabolic Engineering, Vol. 32
Biosensor-driven adaptive laboratory evolution of l-valine production in Corynebacterium glutamicum
journal, November 2015
- Mahr, Regina; Gätgens, Cornelia; Gätgens, Jochem
- Metabolic Engineering, Vol. 32
Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction
journal, January 2013
- Resch, Michael G.; Donohoe, Bryon S.; Baker, John O.
- Energy & Environmental Science, Vol. 6, Issue 6
Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
journal, September 2008
- Shaw, A. J.; Podkaminer, K. K.; Desai, S. G.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 37, p. 13769-13774
Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum
journal, August 2011
- Brown, S. D.; Guss, A. M.; Karpinets, T. V.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 33
Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
journal, June 2014
- Chung, D.; Cha, M.; Guss, A. M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936
Mutator action by Escherichia coli strains carrying dnaE mutations
journal, September 1977
- Sevastopoulos, C. G.; Glaser, D. A.
- Proceedings of the National Academy of Sciences, Vol. 74, Issue 9
Stability of Glutamine and Pyroglutamic Acid under Model System Conditions: Influence of Physical and Technological Factors
journal, November 1987
- Airaudo, Charles B.; Gayte-Sorbier, AndrÉ; Armand, Paul
- Journal of Food Science, Vol. 52, Issue 6
Physiology, Genomics, and Pathway Engineering of an Ethanol-Tolerant Strain of Clostridium phytofermentans
journal, June 2015
- Tolonen, Andrew C.; Zuroff, Trevor R.; Ramya, Mohandass
- Applied and Environmental Microbiology, Vol. 81, Issue 16
Effects of Stirring and Hydrogen on Fermentation Products of Clostridium thermocellum
journal, January 1988
- Lamed, R. J.; Lobos, J. H.; Su, T. M.
- Applied and Environmental Microbiology, Vol. 54, Issue 5
Improvement of Cellulolytic Properties of Clostridium cellulolyticum by Metabolic Engineering
journal, January 2002
- Guedon, E.; Desvaux, M.; Petitdemange, H.
- Applied and Environmental Microbiology, Vol. 68, Issue 1
Assembly of a novel biosynthetic pathway for gentamicin B production in Micromonospora echinospora
journal, January 2016
- Ni, Xianpu; Sun, Zhenpeng; Gu, Yawen
- Microbial Cell Factories, Vol. 15, Issue 1
Enhanced heterologous protein productivity by genome reduction in Lactococcus lactis NZ9000
journal, January 2017
- Zhu, Duolong; Fu, Yuxin; Liu, Fulu
- Microbial Cell Factories, Vol. 16, Issue 1
The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading
journal, October 2014
- Holwerda, Evert K.; Thorne, Philip G.; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 7, Issue 1
Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum
journal, January 2015
- Biswas, Ranjita; Zheng, Tianyong; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Anaerobic detoxification of acetic acid in a thermophilic ethanologen
journal, May 2015
- Shaw, A. Joe; Miller, Bethany B.; Rogers, Stephen R.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment
journal, January 2016
- Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood
journal, June 2016
- Herring, Christopher D.; Kenealy, William R.; Joe Shaw, A.
- Biotechnology for Biofuels, Vol. 9, Issue 1
Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria
journal, November 2017
- Eminoğlu, Ayşenur; Murphy, Sean Jean-Loup; Maloney, Marybeth
- Biotechnology for Biofuels, Vol. 10, Issue 1
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass
journal, January 2019
- Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad
- Biotechnology for Biofuels, Vol. 12, Issue 1
Engineering Corynebacterium glutamicum triggers glutamic acid accumulation in biotin-rich corn stover hydrolysate
journal, April 2019
- Wen, Jingbai; Bao, Jie
- Biotechnology for Biofuels, Vol. 12, Issue 1
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