Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements

Atha, Donald H.; Nagy, Amber; Steinbrück, Andrea; Dennis, Allison M.; Hollingsworth, Jennifer A.; Dua, Varsha; Iyer, Rashi; Nelson, Bryant C.

doi:10.1186/s12951-017-0312-3

Title: Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements

Journal Article · 09 November 2017 · Journal of Nanobiotechnology

DOI: https://doi.org/10.1186/s12951-017-0312-3 · OSTI ID:1626901

Atha, Donald H. ^[1]; Nagy, Amber ^[2]; Steinbrück, Andrea ^[3]; Dennis, Allison M. ^[4]; Hollingsworth, Jennifer A. ^[5]; Dua, Varsha ^[1]; Iyer, Rashi ^[6]; Nelson, Bryant C. ^[1]

National Inst. of Standards and Technology (NIST), Gaithersburg, MD (United States). Biosystems and Biomaterials Division
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division; Boston Univ., MA (United States). Department of Biomedical Engineering and Division of Materials Science and Engineering
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies, Materials Physics & Applications Division
Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Defense Systems and Analysis Division

When evaluating the toxicity of engineered nanomaterials (ENMS) it is important to use multiple bioassays based on different mechanisms of action. In this regard we evaluated the use of gene expression and common cytotoxicity measurements using as test materials, two selected nanoparticles with known differences in toxicity, 5 nm mercaptoundecanoic acid (MUA)-capped InP and CdSe quantum dots (QDs). We tested the effects of these QDs at concentrations ranging from 0.5 to 160 µg/mL on cultured normal human bronchial epithelial (NHBE) cells using four common cytotoxicity assays: the dichlorofluorescein assay for reactive oxygen species (ROS), the lactate dehydrogenase assay for membrane viability (LDH), the mitochondrial dehydrogenase assay for mitochondrial function, and the Comet assay for DNA strand breaks.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC52-06NA25396

OSTI ID:: 1626901

Journal Information:: Journal of Nanobiotechnology, Vol. 15, Issue 1; ISSN 1477-3155

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements Atha, Donald; Nagy, Amber; Steinbrück, Andrea https://doi.org/10.6084/m9.figshare.c.3926995.v1 The current record is supplemented by this collection	collection	January 2017
MOESM1 of Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements Atha, Donald; Nagy, Amber; SteinbrĂźck, Andrea https://doi.org/10.6084/m9.figshare.c.3926995_d1.v1 The current record is supplemented by this text	text	January 2017
MOESM1 of Quantifying engineered nanomaterial toxicity: comparison of common cytotoxicity and gene expression measurements Atha, Donald; Nagy, Amber; SteinbrĂźck, Andrea https://doi.org/10.6084/m9.figshare.c.3926995_d1 The current record is supplemented by this text	text	January 2017