Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

Sainathrao, Shilpakala; Mohan, Ketha; Atreya, Chintamani

doi:10.1186/1472-6750-9-67

Title: Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

Journal Article · 22 July 2009 · BMC Biotechnology (Online)

DOI: https://doi.org/10.1186/1472-6750-9-67 · OSTI ID:1626542

Sainathrao, Shilpakala ^[1]; Mohan, Ketha ^[2]; Atreya, Chintamani ^[2]

U.S. Food and Drug Administration (FDA), Bethesda, MD (United States). Center for Biologics Evaluation and Research. Lab. of Cellular Hematology. Section of Cell biology; DOE/OSTI
U.S. Food and Drug Administration (FDA), Bethesda, MD (United States). Center for Biologics Evaluation and Research. Lab. of Cellular Hematology. Section of Cell biology

Background: Previous reports of site-directed deletion analysis on gamma (γ)-phage lysin protein (PlyG) have demonstrated that removal of a short amino acid sequence in the C-terminal region encompassing a 10-amino acid motif (190LKMTADFILQ199) abrogates its binding activity specific to the cell wall of Bacillus anthracis. Whether short synthetic peptides representing the10-amino acid PlyG putative binding motif flanked by surrounding N- and C-terminal residues also selectively bind to the bacterial cell wall has not been evaluated. If such peptides do demonstrate selective binding to the cell wall, they could serve as bio-probes towards developing detection technologies for B. anthracis. Furthermore, by using B. anthracis (Sterne, 34F2), an animal vaccine and B. cereus4342, a γ-phage susceptible rare strain as surrogates of B. anthracis, development of proof-of-concepts for B. anthracis are feasible. Results: Using four different methods, we evaluated six synthetic peptides representing the putative binding motif including flanking sequences (PlyG-P1 through P6) for the bacterial cell wall binding capacity. Our analysis identified PlyG-P1, PlyG-P3 and PlyG-P5 to have binding capability to both B. anthracis (Sterne, 34F2) and B. cereus-4342. The peptides however did not bind to B. cereus11778, B. thuringiensis, and B. cereus-10876 suggesting their specificity for B. anthracis-Sterne and B. cereus-4342. PlyG-P3 in combination with fluorescent light microscopy detected even a single bacterium in plasma spiked with the bacteria. Conclusion: Overall, these studies illustrate that the short 10-amino acid sequence 'LKMTADFILQ' in fact is a stand-alone bacterial cell wall-binding motif of PlyG. In principle, synthetic peptides PlyG-P1, PlyG-P3 and PlyG-P5, especially PlyG-P3 coupled with Qdotnanocrystals are useful as high-sensitivity bio-probes in developing detection technologies for B. anthracis.

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Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: SC0014664

OSTI ID:: 1626542

Journal Information:: BMC Biotechnology (Online), Journal Name: BMC Biotechnology (Online) Journal Issue: 1 Vol. 9; ISSN 1472-6750

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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High-sensitivity bacterial detection using biotin-tagged phage and quantum-dot nanocomplexes Edgar, Rotem; McKinstry, Michael; Hwang, Jeeseong Proceedings of the National Academy of Sciences, Vol. 103, Issue 13 https://doi.org/10.1073/pnas.0601211103	journal	March 2006
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A Peptide Derived from Phage Display Library Exhibits Antibacterial Activity against E. coli and Pseudomonas aeruginosa Sainath Rao, Shilpakala; Mohan, Ketha V. K.; Atreya, Chintamani D. PLoS ONE, Vol. 8, Issue 2 https://doi.org/10.1371/journal.pone.0056081	journal	February 2013
Bacillus anthracis gamma phage lysis among soil bacteria: an update on test specificity Kolton, Cari B.; Podnecky, Nicole L.; Shadomy, Sean V. BMC Research Notes, Vol. 10, Issue 1 https://doi.org/10.1186/s13104-017-2919-8	journal	November 2017
Bacteriophage biosensors for antibiotic-resistant bacteria Sorokulova, Irina; Olsen, Eric; Vodyanoy, Vitaly Expert Review of Medical Devices, Vol. 11, Issue 2 https://doi.org/10.1586/17434440.2014.882767	journal	November 2013
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Phage-based platforms for the clinical detection of human bacterial pathogens Schofield, David; Sharp, Natasha J.; Westwater, Caroline Bacteriophage, Vol. 2, Issue 2 https://doi.org/10.4161/bact.19274	journal	April 2012

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Title: Gamma-phage lysin PlyG sequence-based synthetic peptides coupled with Qdot-nanocrystals are useful for developing detection methods for Bacillus anthracis by using its surrogates, B. anthracis-Sterne and B. cereus-4342

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