Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

Puckette, Michael; Clark, Benjamin A.; Smith, Justin D.; Turecek, Traci; Martel, Erica; Gabbert, Lindsay; Pisano, Melia; Hurtle, William; Pacheco, Juan M.; Barrera, José; Neilan, John G.; Rasmussen, Max

doi:10.1128/jvi.00924-17

Title: Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development

Journal Article · 27 July 2017 · Journal of Virology

DOI: https://doi.org/10.1128/jvi.00924-17 · OSTI ID:1626072

Puckette, Michael ^[1]; Clark, Benjamin A. ^[2]; Smith, Justin D. ^[2]; Turecek, Traci ^[3]; Martel, Erica ^[4]; Gabbert, Lindsay ^[3]; Pisano, Melia ^[3]; Hurtle, William ^[5]; Pacheco, Juan M. ^[4]; Barrera, José ^[6]; Neilan, John G. ^[5]; Rasmussen, Max ^[5]

US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center; Leidos, Inc., Greenport, NY (United States). Plum Island Animal Disease Center; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States). Plum Island Animal Disease Center Research Participation Program
Leidos, Inc., Greenport, NY (United States). Plum Island Animal Disease Center; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States). Plum Island Animal Disease Center Research Participation Program
Leidos, Inc., Greenport, NY (United States). Plum Island Animal Disease Center
Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States). Plum Island Animal Disease Center Research Participation Program
US Dept. of Homeland Security (DHS), Greenport, NY (United States). Plum Island Animal Disease Center;
Leidos, Inc., Greenport, NY (United States). Plum Island Animal Disease Center;

The foot-and-mouth disease virus (FMDV) afflicts livestock in more than 80 countries, limiting food production and global trade. Production of foot-and-mouth disease (FMD) vaccines requires cytosolic expression of the FMDV 3C protease to cleave the P1 polyprotein into mature capsid proteins, but the FMDV 3C protease is toxic to host cells. To identify less-toxic isoforms of the FMDV 3C protease, we screened 3C mutants for increased transgene output in comparison to wild-type 3C using aGaussialuciferase reporter system. The novel point mutation 3C(L127P) increased yields of recombinant FMDV subunit proteins in mammalian and bacterial cells expressing P1-3C transgenes and retained the ability to process P1 polyproteins from multiple FMDV serotypes. The 3C(L127P) mutant produced crystalline arrays of FMDV-like particles in mammalian and bacterial cells, potentially providing a practical method of rapid, inexpensive FMD vaccine production in bacteria.

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Research Organization:: Oak Ridge Associated Univ., Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-06OR23100; HSHQDC-14-F-00035; HSHQPM-14-X00001

OSTI ID:: 1626072

Journal Information:: Journal of Virology, Vol. 91, Issue 22; ISSN 0022-538X

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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pAd5-Blue: Direct Ligation System for Engineering Recombinant Adenovirus Constructs Moraes, Mauro P.; Mayr, Gregory A.; Grubman, Marvin J. BioTechniques, Vol. 31, Issue 5 https://doi.org/10.2144/01315st05	journal	November 2001

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Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant Cui, Xuemei; Wang, Yong; Maqbool, Babar Vaccines, Vol. 7, Issue 4 https://doi.org/10.3390/vaccines7040143	journal	October 2019
Early IgG Response to Foot and Mouth Disease Vaccine Formulated with a Vegetable Oil Adjuvant Cui, Xuemei; Wang, Yong; Maqbool, Babar Vaccines, Vol. 7, Issue 4 https://doi.org/10.3390/vaccines7040143	journal	October 2019

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
Virology
3C protease
StopGo translation
Escherichia coli
Gaussia luciferase
VLP
adenoviruses
foot-and-mouth disease virus
in vivo
in vivo expression technology
translational interrupter
vaccines
virus-like particles