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Title: The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis

Abstract

Phototrophic organisms are superbly adapted to different light environments but often must acclimate to challenging competition for visible light wavelengths in their niches. Some cyanobacteria overcome this challenge by expressing paralogous photosynthetic proteins and by synthesizing and incorporating ~8% chlorophyll f into their Photosystem I (PSI) complexes, enabling them to grow under far-red light (FRL). We solved the structure of FRL-acclimated PSI from the cyanobacteriumFischerella thermalisPCC 7521 by single-particle, cryo–electron microscopy to understand its structural and functional differences. Four binding sites occupied by chlorophyll f are proposed. Subtle structural changes enable FRL-adapted PSI to extend light utilization for oxygenic photosynthesis to nearly 800 nm. This structure provides a platform for understanding FRL-driven photosynthesis and illustrates the robustness of adaptive and acclimation mechanisms in nature.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2]; ORCiD logo [3]; ORCiD logo [4];  [4]; ORCiD logo [5]; ORCiD logo [1]; ORCiD logo [6]
  1. Arizona State Univ., Mesa, AZ (United States). Biodesign Ctr for Applied Structural Discovery; Arizona State Univ., Mesa, AZ (United States). School of Molecular Sciences
  2. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology
  3. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology; Pennsylvania State Univ., University Park, PA (United States). Intercollege Graduate Program in Plant Biology
  4. Arizona State Univ., Mesa, AZ (United States). Biodesign Ctr for Applied Structural Discovery
  5. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology; Pennsylvania State Univ., University Park, PA (United States). Dept. of Chemistry
  6. Pennsylvania State Univ., University Park, PA (United States). Dept. of Biochemistry and Molecular Biology; Pennsylvania State Univ., University Park, PA (United States). Intercollege Graduate Program in Plant Biology; Montana State Univ., Bozeman, MT (United States). Dept. of Chemistry and Biochemistry
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1626049
Grant/Contract Number:  
SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Science Advances
Additional Journal Information:
Journal Volume: 6; Journal Issue: 6; Journal ID: ISSN 2375-2548
Publisher:
AAAS
Country of Publication:
United States
Language:
English
Subject:
Science & Technology - Other Topics

Citation Formats

Gisriel, Christopher, Shen, Gaozhong, Kurashov, Vasily, Ho, Ming-Yang, Zhang, Shangji, Williams, Dewight, Golbeck, John H., Fromme, Petra, and Bryant, Donald A.. The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis. United States: N. p., 2020. Web. https://doi.org/10.1126/sciadv.aay6415.
Gisriel, Christopher, Shen, Gaozhong, Kurashov, Vasily, Ho, Ming-Yang, Zhang, Shangji, Williams, Dewight, Golbeck, John H., Fromme, Petra, & Bryant, Donald A.. The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis. United States. https://doi.org/10.1126/sciadv.aay6415
Gisriel, Christopher, Shen, Gaozhong, Kurashov, Vasily, Ho, Ming-Yang, Zhang, Shangji, Williams, Dewight, Golbeck, John H., Fromme, Petra, and Bryant, Donald A.. Sat . "The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis". United States. https://doi.org/10.1126/sciadv.aay6415. https://www.osti.gov/servlets/purl/1626049.
@article{osti_1626049,
title = {The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis},
author = {Gisriel, Christopher and Shen, Gaozhong and Kurashov, Vasily and Ho, Ming-Yang and Zhang, Shangji and Williams, Dewight and Golbeck, John H. and Fromme, Petra and Bryant, Donald A.},
abstractNote = {Phototrophic organisms are superbly adapted to different light environments but often must acclimate to challenging competition for visible light wavelengths in their niches. Some cyanobacteria overcome this challenge by expressing paralogous photosynthetic proteins and by synthesizing and incorporating ~8% chlorophyll f into their Photosystem I (PSI) complexes, enabling them to grow under far-red light (FRL). We solved the structure of FRL-acclimated PSI from the cyanobacteriumFischerella thermalisPCC 7521 by single-particle, cryo–electron microscopy to understand its structural and functional differences. Four binding sites occupied by chlorophyll f are proposed. Subtle structural changes enable FRL-adapted PSI to extend light utilization for oxygenic photosynthesis to nearly 800 nm. This structure provides a platform for understanding FRL-driven photosynthesis and illustrates the robustness of adaptive and acclimation mechanisms in nature.},
doi = {10.1126/sciadv.aay6415},
journal = {Science Advances},
number = 6,
volume = 6,
place = {United States},
year = {2020},
month = {2}
}

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