High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP

Schumacher, Maria A.; Ohashi, Tomoo; Corbin, Lauren; Erickson, Harold P.

doi:10.1107/s2053230x20001132

Title: High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP

Journal Article · 01 February 2020 · Acta Crystallographica. Section F, Structural Biology Communications

DOI: https://doi.org/10.1107/s2053230x20001132 · OSTI ID:1625835

Schumacher, Maria A. ^[1]; Ohashi, Tomoo ^[2]; Corbin, Lauren ^[3]; Erickson, Harold P. ^[4]

Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Biochemistry; DOE/OSTI
Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Cell Biology
Duke Univ., Durham, NC (United States). Dept. of Biomedical Engineering
Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Biochemistry; Duke Univ., Durham, NC (United States). School of Medicine. Dept. of Cell Biology

Bacterial cytokinesis is mediated by the Z-ring, which is formed by the prokaryotic tubulin homolog FtsZ. Recent data indicate that the Z-ring is composed of small patches of FtsZ protofilaments that travel around the bacterial cell by treadmilling. Treadmilling involves a switch from a relaxed (R) state, favored for monomers, to a tense (T) conformation, which is favored upon association into filaments. The R conformation has been observed in numerous monomeric FtsZ crystal structures and the T conformation inStaphylococcus aureusFtsZ crystallized as assembled filaments. However, whileEscherichia colihas served as a main model system for the study of the Z-ring and the associated divisome, a structure has not yet been reported forE. coliFtsZ. To address this gap, structures were determined of theE. coliFtsZ mutant FtsZ(L178E) with GDP and GTP bound to 1.35 and 1.40 Å resolution, respectively. TheE. coliFtsZ(L178E) structures both crystallized as straight filaments with subunits in the R conformation. These high-resolution structures can be employed to facilitate experimental cell-division studies and their interpretation inE. coli.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625835

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 2 Vol. 76; ISSN ACSFEN; ISSN 2053-230X

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: High-resolution crystal structures of Escherichia coli FtsZ bound to GDP and GTP

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