Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster

Journal Article · 23 September 2010 · Acta Crystallographica. Section F

DOI: https://doi.org/10.1107/s1744309110037619 · OSTI ID:1625806

Han, Gye Won ^[1]; Yang, Xiang-Lei ^[1]; McMullan, Daniel ^[2]; Chong, Yeeting E. ^[1]; Krishna, S. Sri ^[3]; Rife, Christopher L. ^[4]; Weekes, Dana ^[5]; Brittain, Scott M. ^[6]; Abdubek, Polat ^[2]; Ambing, Eileen ^[2]; Astakhova, Tamara ^[7]; Axelrod, Herbert L. ^[4]; Carlton, Dennis ^[1]; Caruthers, Jonathan ^[4]; Chiu, Hsiu-Ju ^[4]; Clayton, Thomas ^[1]; Duan, Lian ^[7]; Feuerhelm, Julie ^[2]; Grant, Joanna C. ^[2]; Grzechnik, Slawomir K. ^[7] ; Jaroszewski, Lukasz ^[3]; Jin, Kevin K. ^[4]; Klock, Heath E. ^[2]; Knuth, Mark W. ^[2]; Kumar, Abhinav ^[4]; Marciano, David ^[1]; Miller, Mitchell D. ^[4]; Morse, Andrew T. ^[7]; Nigoghossian, Edward ^[2]; Okach, Linda ^[2]; Paulsen, Jessica ^[2]; Reyes, Ron ^[4]; van den Bedem, Henry ^[4]; White, Aprilfawn ^[2]; Wolf, Guenter ^[4]; Xu, Qingping ^[4]; Hodgson, Keith O. ^[8]; Wooley, John ^[7]; Deacon, Ashley M. ^[4]; Godzik, Adam ^[3]; Lesley, Scott A. ^[2]; Elsliger, Marc-André ^[1]; Schimmel, Paul ^[1]; Wilson, Ian A. ^[1]

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1. The Scripps Research Inst., La Jolla, CA (United States)
2. The Scripps Research Inst., La Jolla, CA (United States); Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States)
3. The Scripps Research Inst., La Jolla, CA (United States); Univ. of California, San Diego, La Jolla, CA (United States); Sanford–Burnham Medical Research Inst., La Jolla, CA (United States)
4. The Scripps Research Inst., La Jolla, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)
5. The Scripps Research Inst., La Jolla, CA (United States); Sanford–Burnham Medical Research Inst., La Jolla, CA (United States)
6. Genomics Inst. of the Novartis Research Foundation, San Diego, CA (United States)
7. The Scripps Research Inst., La Jolla, CA (United States); Univ. of California, San Diego, La Jolla, CA (United States)
8. The Scripps Research Inst., La Jolla, CA (United States); SLAC National Accelerator Lab., Menlo Park, CA (United States)

A novel aminoacyl-tRNA synthetase that contains an iron–sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron–sulfur [4Fe–4S] cluster bound to the tRNA anticodon-binding (TAB) domain and an l-tryptophan ligand in the active site. None of the other T. maritima aminoacyltRNA synthetases (AARSs) contain this [4Fe–4S] cluster-binding motif (C-x22- C-x6-C-x2-C). It is speculated that the iron–sulfur cluster contributes to the stability of TmTrpRS and could play a role in the recognition of the anticodon.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); USDOE Office of Science (SC), Basic Energy Sciences (BES); National Center for Research Resources (NCRR)
• Grant/Contract Number: AC02-05CH11231; U54 GM074898
• OSTI ID: 1625806
• Journal Information: Acta Crystallographica. Section F, Vol. 66, Issue 10; ISSN 1744-3091
• Publisher: International Union of CrystallographyCopyright Statement
• Country of Publication: United States
• Language: English