Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase

Sherrer, R. Lynn; Ho, Joanne M.  L.; Söll, Dieter

doi:10.1093/nar/gkn036

Title: Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNA^Sec kinase

Abstract

Selenocysteine (Sec) biosynthesis in archaea and eukaryotes requires three steps: serylation of tRNA^Sec by seryl-tRNA synthetase (SerRS), phosphorylation of Ser-tRNA^Sec by O-phosphoseryl-tRNA^Sec kinase (PSTK), and conversion of O-phosphoseryl-tRNA^Sec (Sep-tRNA^Sec) by SeptRNA:Sec-tRNA synthase (SepSecS) to SectRNA^Sec. Although SerRS recognizes both tRNA^Sec and tRNA^Ser species, PSTK must discriminate SertRNA^Sec from Ser-tRNA^Ser. Based on a comparison of the sequences and secondary structures of archaeal tRNA^Sec and tRNA^Ser, we introduced mutations into Methanococcus maripaludis tRNA^Sec to investigate how Methanocaldococcus jannaschii PSTK distinguishes tRNA^Sec from tRNA^Ser. Unlike eukaryotic PSTK, the archaeal enzyme was found to recognize the acceptor stem rather than the length and secondary structure of the D-stem. While the D-arm and T-loop provide minor identity elements, the acceptor stem base pairs G2-C71 and C3-G70 in tRNA^Sec were crucial for discrimination from tRNA^Ser. Furthermore, the A5-U68 base pair in tRNA^Ser has some antideterminant properties for PSTK. Transplantation of these identity elements into the tRNA^Ser_UGA scaffold resulted in phosphorylation of the chimeric Ser-tRNA. The chimera was able to stimulate the ATPase activity of PSTK albeit at a lower level than tRNA^Sec, whereas tRNA^Ser did not. Additionally, the seryl moiety of Ser-tRNA^Sec is not required for enzyme recognition, as PSTK efficiently phosphorylated Thr-tRNA^Sec.

Authors:

Sherrer, R. Lynn ^[1]; Ho, Joanne M. L. ^[1]; Söll, Dieter ^[2]

Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics and Biochemistry
Yale Univ., New Haven, CT (United States). Dept. of Molecular Biophysics and Biochemistry; Yale Univ., New Haven, CT (United States). Dept. of Chemistry

Publication Date:: Mon Feb 11 00:00:00 EST 2008

Research Org.:: Yale Univ., New Haven, CT (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH)

OSTI Identifier:: 1625437

Grant/Contract Number:: GM22854; GM075602

Resource Type:: Accepted Manuscript

Journal Name:: Nucleic Acids Research

Additional Journal Information:: Journal Volume: 36; Journal Issue: 6; Journal ID: ISSN 0305-1048

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Biochemistry & Molecular Biology

Citation Formats


                    Sherrer, R. Lynn, Ho, Joanne M.  L., and Söll, Dieter. Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase.  United States: N. p., 2008. 
Web.  doi:10.1093/nar/gkn036.

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                    Sherrer, R. Lynn, Ho, Joanne M.  L., & Söll, Dieter. Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase.  United States.  https://doi.org/10.1093/nar/gkn036

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                    Sherrer, R. Lynn, Ho, Joanne M.  L., and Söll, Dieter. Mon .  
"Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase".  United States.  https://doi.org/10.1093/nar/gkn036.  https://www.osti.gov/servlets/purl/1625437.

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@article{osti_1625437,

  title        = {Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase},

  author       = {Sherrer, R. Lynn and Ho, Joanne M.  L. and Söll, Dieter},

  abstractNote = {Selenocysteine (Sec) biosynthesis in archaea and eukaryotes requires three steps: serylation of tRNASec by seryl-tRNA synthetase (SerRS), phosphorylation of Ser-tRNASec by O-phosphoseryl-tRNASec kinase (PSTK), and conversion of O-phosphoseryl-tRNASec (Sep-tRNASec) by SeptRNA:Sec-tRNA synthase (SepSecS) to SectRNASec. Although SerRS recognizes both tRNASec and tRNASer species, PSTK must discriminate SertRNASec from Ser-tRNASer. Based on a comparison of the sequences and secondary structures of archaeal tRNASec and tRNASer, we introduced mutations into Methanococcus maripaludis tRNASec to investigate how Methanocaldococcus jannaschii PSTK distinguishes tRNASec from tRNASer. Unlike eukaryotic PSTK, the archaeal enzyme was found to recognize the acceptor stem rather than the length and secondary structure of the D-stem. While the D-arm and T-loop provide minor identity elements, the acceptor stem base pairs G2-C71 and C3-G70 in tRNASec were crucial for discrimination from tRNASer. Furthermore, the A5-U68 base pair in tRNASer has some antideterminant properties for PSTK. Transplantation of these identity elements into the tRNASerUGA scaffold resulted in phosphorylation of the chimeric Ser-tRNA. The chimera was able to stimulate the ATPase activity of PSTK albeit at a lower level than tRNASec, whereas tRNASer did not. Additionally, the seryl moiety of Ser-tRNASec is not required for enzyme recognition, as PSTK efficiently phosphorylated Thr-tRNASec.},

  doi          = {10.1093/nar/gkn036},

  journal      = {Nucleic Acids Research},

  number       = 6,

  volume       = 36,

  place        = {United States},

  year         = {Mon Feb 11 00:00:00 EST 2008},

  month        = {Mon Feb 11 00:00:00 EST 2008}

}

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Kaiser, Jens T.; Gromadski, Kirill; Rother, Michael
Biochemistry, Vol. 44, Issue 40
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Carlsen, E. N.; Trelle, G. J.; Schjeide, O. A.
Nature, Vol. 202, Issue 4936
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Crystal structure of yeast tRNAAsp
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Moras, D.; Comarmond, M. B.; Fischer, J.
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Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine
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Nature, Vol. 331, Issue 6158
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Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein
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Forchhammer, Karl; Leinfelder, Walfred; Böck, August
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Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition
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Yuan, J.; Palioura, S.; Salazar, J. C.
Proceedings of the National Academy of Sciences, Vol. 103, Issue 50, p. 18923-18927
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A Specific Hepatic Transfer RNA for Phosphoserine
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Proceedings of the National Academy of Sciences, Vol. 72, Issue 11
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Hatfield, D.; Diamond, A.; Dudock, B.
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Fagegaltier, D.
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Compilation of tRNA sequences and sequences of tRNA genes
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Sprinzl, M.; Dank, N.; Nock, S.
Nucleic Acids Research, Vol. 19, Issue suppl
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Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles
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Quigley, G. J.; Seeman, N. C.; Wang, A. H. -J.
Nucleic Acids Research, Vol. 2, Issue 12
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Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA ^Sec
journal, January 1993

Sturchler, Christine; Westhof, Eric; Carbon, Philippe
Nucleic Acids Research, Vol. 21, Issue 5
DOI: 10.1093/nar/21.5.1073

Universal rules and idiosyncratic features in tRNA identity
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Giege, R.; Sissler, M.; Florentz, C.
Nucleic Acids Research, Vol. 26, Issue 22
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Sharp, Stephen J.; Stewart, Thomas S.
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DOI: 10.1093/nar/4.7.2123

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Laslett, Dean; Canback, Bjorn
Nucleic Acids Research, Vol. 32, Issue 1, p. 11-16
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The UCSC Archaeal Genome Browser
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Schneider, K. L.
Nucleic Acids Research, Vol. 34, Issue 90001
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A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine
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Bailly, Marc; Giannouli, Stamatina; Blaise, Mickael
Nucleic Acids Research, Vol. 34, Issue 21
DOI: 10.1093/nar/gkl622

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
journal, January 2008

Sherrer, R. Lynn; O’Donoghue, Patrick; Söll, Dieter
Nucleic Acids Research, Vol. 36, Issue 4, p. 1247-1259
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Biosynthesis of selenocysteine on its tRNA in eukaryotes
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Xu, Xue‐Ming; Carlson, Bradley A.; Mix, Heiko
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Hatfield, D. L.; Lee, B. J.; Price, N. M.
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Oshikane, H.
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Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation

Journal Article Sherrer, R. Lynn ; O’Donoghue, Patrick ; Söll, Dieter - Nucleic Acids Research

Selenocysteine (Sec)-decoding archaea and eukaryotes employ a unique route of Sec-tRNA^Sec synthesis in which O-phosphoseryl-tRNA^Sec kinase (PSTK) phosphorylates Ser-tRNASec to produce the O-phosphoseryl-tRNA^Sec (Sep-tRNA^Sec) substrate that Sep-tRNA:Sec-tRNA synthase (SepSecS) converts to Sec-tRNA^Sec. This study presents a biochemical characterization of Methanocaldococcus jannaschii PSTK, including kinetics of Sep-tRNA^Sec formation (K_m for Ser-tRNA^Sec of 40 nM and ATP of 2.6 mM). PSTK binds both Ser-tRNA^Sec and tRNA^Sec with high affinity (K_d values of 53 nM and 39 nM, respectively). The ATPase activity of PSTK may be activated via an induced fit mechanism in which binding of tRNA^Sec specifically stimulates hydrolysis. Albeit with lowermore »« less
https://doi.org/10.1093/nar/gkm1134

Full Text Available
Structural basis for the tRNA-dependent activation of the terminal complex of selenocysteine synthesis in humans

Journal Article Puppala, Anupama K. ; Castillo Suchkou, Jennifer ; French, Rachel L. ; ... - Nucleic Acids Research

Abstract O-Phosphoseryl-tRNASec selenium transferase (SepSecS) catalyzes the terminal step of selenocysteine (Sec) synthesis in archaea and eukaryotes. How the Sec synthetic machinery recognizes and discriminates tRNASec from the tRNA pool is essential to the integrity of the selenoproteome. Previously, we suggested that SepSecS adopts a competent conformation that is pre-ordered for catalysis. Herein, using high-resolution X-ray crystallography, we visualized tRNA-dependent conformational changes in human SepSecS that may be a prerequisite for achieving catalytic competency. We show that tRNASec binding organizes the active sites of the catalytic protomer, while stabilizing the N- and C-termini of the non-catalytic protomer. Binding of largemore »« less
https://doi.org/10.1093/nar/gkad182
Formation of m ^{$$\texttt{2}$$} G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14

Journal Article Menezes, Sheena ; Gaston, Kirk W. ; Krivos, Kady L. ; ... - Nucleic Acids Research

The modified nucleosides N² -methylguanosine and Nmore »« less
https://doi.org/10.1093/nar/gkr475

Full Text Available
The Human SepSecS-tRNA[superscript Sec] Complex Reveals the Mechanism of Selenocysteine Formation

Journal Article Palioura, Sotiria ; Sherrer, R Lynn ; Steitz, Thomas A ; ... - Science

Selenocysteine is the only genetically encoded amino acid in humans whose biosynthesis occurs on its cognate transfer RNA (tRNA). O-Phosphoseryl-tRNA:selenocysteinyl-tRNA synthase (SepSecS) catalyzes the final step of selenocysteine formation by a poorly understood tRNA-dependent mechanism. The crystal structure of human tRNA{sup Sec} in complex with SepSecS, phosphoserine, and thiophosphate, together with in vivo and in vitro enzyme assays, supports a pyridoxal phosphate-dependent mechanism of Sec-tRNA{sup Sec} formation. Two tRNA{sup Sec} molecules, with a fold distinct from other canonical tRNAs, bind to each SepSecS tetramer through their 13-base pair acceptor-T{Upsilon}C arm (where {Upsilon} indicates pseudouridine). The tRNA binding is likely tomore »« less
https://doi.org/10.1126/science.1173755
Identification of a selenocysteyl-tRNA(Ser) in mammalian cells that recognizes the nonsense codon, UGA

Journal Article Lee, B J ; Worland, P J ; Davis, J N ; ... - J. Biol. Chem.; (United States)

The presence of a unique opal suppressor seryl-tRNA in higher vertebrates which is converted to phosphoseryl-tRNA has been known for several years, but its function has been uncertain. In the present study, we demonstrate that this tRNA species also occurs in vivo as selenocysteyl-tRNA(Ser) suggesting that it functions both as a carrier molecule upon which selenocysteine is synthesized and as a direct selenocysteine donor to a growing polypeptide chain in response to specific UGA codons. (75Se)Seleno(3H)cysteyl-tRNA(Ser) formed by administering 75Se and (3H)serine to rat mammary tumor cells (TMT-081-MS) in culture was isolated from the cell extract. The amino acid attachedmore »« less

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Title: Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNASec kinase

Abstract

Citation Formats

Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA Asn journal, January 2007

Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA Sec journal, January 1993

Universal rules and idiosyncratic features in tRNA identity journal, November 1998

Structure of yeast phenylalanine transfer RNA at 2.5 A resolution. journal, November 1975

Modulation of tRNAAla identity by inorganic pyrophosphatase journal, April 2002

Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code journal, June 2006

The long extra arms of human tRNA(Ser)Sec and tRNASer function as major identity elements for serylation in an orientation-dependent, but not sequence-specific manner journal, January 1993

Structural and Functional Investigation of a Putative Archaeal Selenocysteine Synthase † , ‡ journal, October 2005

Solution Structure of Selenocysteine-inserting tRNASec from Escherichia coli journal, May 1993

The UCSC Archaeal Genome Browser journal, January 2006

ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences journal, January 2004

Transfer Ribonucleic Acids journal, June 1964

The length and the secondary structure of the D-stem of human selenocysteine tRNA are the major identity determinants for serine phosphorylation. journal, January 1994

The characterization of phosphoseryl tRNA from lactating bovine mammary gland journal, January 1977

The unusual methanogenic seryl‐tRNA synthetase recognizes tRNASer species from all three kingdoms of life journal, January 2004

Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition journal, May 2006

Compilation of tRNA sequences and sequences of tRNA genes journal, April 1991

Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein journal, November 1989

RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea journal, December 2006

Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation journal, September 2000

Structural compensation in an archaeal selenocysteine transfer RNA journal, July 1999

A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine journal, October 2006

Purification and properties of suppressor seryl-tRNA:ATP phosphotransferase from bovine liver journal, April 1984

Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase journal, August 2004

Amino Acid Discrimination by a Class I Aminoacyl-tRNA Synthetase Specified by Negative Determinants journal, April 2003

Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA. journal, October 1982

Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes journal, December 2006

Crystal structure of yeast tRNAAsp journal, December 1980

Selenocysteyl-tRNA occurs in the diatom Thalassiosira and in the ciliate Tetrahymena journal, May 1991

Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox journal, January 2004

A Specific Hepatic Transfer RNA for Phosphoserine journal, October 1970

Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine journal, February 1988

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation journal, January 2008

Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles journal, January 1975

The Transamidosome: A Dynamic Ribonucleoprotein Particle Dedicated to Prokaryotic tRNA-Dependent Asparagine Biosynthesis journal, October 2007

Identification and characterisation of the selenocysteine-specific translation factor SelB from the archaeon Methanococcus jannaschii 1 1Edited by J. Karn journal, June 2000

Structural compensation in an archaeal selenocysteine transfer RNA journal, July 1999

Purification and properties of suppressor seryl-tRNA:ATP phosphotransferase from bovine liver journal, April 1984

The Transamidosome: A Dynamic Ribonucleoprotein Particle Dedicated to Prokaryotic tRNA-Dependent Asparagine Biosynthesis journal, October 2007

The 9/4 secondary structure of eukaryotic selenocysteine tRNA: More pieces of evidence journal, September 1998

Structural and Functional Investigation of a Putative Archaeal Selenocysteine Synthase † , ‡ journal, October 2005

Transfer Ribonucleic Acids journal, June 1964

Crystal structure of yeast tRNAAsp journal, December 1980

Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine journal, February 1988

Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein journal, November 1989

Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition journal, May 2006

On the road to selenocysteine journal, September 2004

RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea journal, December 2006

A Specific Hepatic Transfer RNA for Phosphoserine journal, October 1970

Structure of yeast phenylalanine transfer RNA at 2.5 A resolution. journal, November 1975

Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA. journal, October 1982

Differential Modes of Transfer RNASer Recognition in Methanosarcina barkeri journal, November 2004

Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation journal, September 2000

Compilation of tRNA sequences and sequences of tRNA genes journal, April 1991

Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles journal, January 1975

Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA Sec journal, January 1993

Universal rules and idiosyncratic features in tRNA identity journal, November 1998

The characterization of phosphoseryl tRNA from lactating bovine mammary gland journal, January 1977

ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences journal, January 2004

The UCSC Archaeal Genome Browser journal, January 2006

A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine journal, October 2006

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation journal, January 2008

Biosynthesis of selenocysteine on its tRNA in eukaryotes journal, April 2007

Selenocysteyl-tRNA occurs in the diatom Thalassiosira and in the ciliate Tetrahymena journal, May 1991

Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code journal, June 2006

Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis journal, May 2011

Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator journal, March 2019

Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s journal, October 2018

tRNA acceptor-stem and anticodon bases embed separate features of amino acid chemistry journal, November 2015

Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology journal, June 2016

tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes journal, August 2021

A tRNA-dependent cysteine biosynthesis enzyme recognizes the selenocysteine-specific tRNA in Escherichia coli journal, May 2010

A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro journal, July 2015

From one amino acid to another: tRNA-dependent amino acid biosynthesis journal, February 2008

Crystal structure of human selenocysteine tRNA journal, August 2009

C-terminal domain of archaeal O -phosphoseryl-tRNA kinase displays large-scale motion to bind the 7-bp D-stem of archaeal tRNA Sec journal, September 2010

Tertiary structure of bacterial selenocysteine tRNA journal, May 2013

Title: Divergence of selenocysteine tRNA recognition by archaeal and eukaryotic O -phosphoseryl-tRNA^Sec kinase

Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA ^Asn
journal, January 2007

Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA ^Sec
journal, January 1993

Universal rules and idiosyncratic features in tRNA identity
journal, November 1998

Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
journal, November 1975

Modulation of tRNA^Ala identity by inorganic pyrophosphatase
journal, April 2002

Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code
journal, June 2006

The long extra arms of human tRNA^(Ser)Sec and tRNA^Ser function as major identity elements for serylation in an orientation-dependent, but not sequence-specific manner
journal, January 1993

Structural and Functional Investigation of a Putative Archaeal Selenocysteine Synthase ^† ^, ^‡
journal, October 2005

Solution Structure of Selenocysteine-inserting tRNASec from Escherichia coli
journal, May 1993

The UCSC Archaeal Genome Browser
journal, January 2006

ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences
journal, January 2004

Transfer Ribonucleic Acids
journal, June 1964

The length and the secondary structure of the D-stem of human selenocysteine tRNA are the major identity determinants for serine phosphorylation.
journal, January 1994

The characterization of phosphoseryl tRNA from lactating bovine mammary gland
journal, January 1977

The unusual methanogenic seryl‐tRNA synthetase recognizes tRNA^Ser species from all three kingdoms of life
journal, January 2004

Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition
journal, May 2006

Compilation of tRNA sequences and sequences of tRNA genes
journal, April 1991

Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein
journal, November 1989

RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
journal, December 2006

Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation
journal, September 2000

Structural compensation in an archaeal selenocysteine transfer RNA
journal, July 1999

A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine
journal, October 2006

Purification and properties of suppressor seryl-tRNA:ATP phosphotransferase from bovine liver
journal, April 1984

Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
journal, August 2004

Amino Acid Discrimination by a Class I Aminoacyl-tRNA Synthetase Specified by Negative Determinants
journal, April 2003

Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.
journal, October 1982

Biosynthesis of Selenocysteine on Its tRNA in Eukaryotes
journal, December 2006

Crystal structure of yeast tRNAAsp
journal, December 1980

Selenocysteyl-tRNA occurs in the diatom Thalassiosira and in the ciliate Tetrahymena
journal, May 1991

Archaeal phylogeny based on proteins of the transcription and translation machineries: tackling the Methanopyrus kandleri paradox
journal, January 2004

A Specific Hepatic Transfer RNA for Phosphoserine
journal, October 1970

Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine
journal, February 1988

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
journal, January 2008

Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles
journal, January 1975

The Transamidosome: A Dynamic Ribonucleoprotein Particle Dedicated to Prokaryotic tRNA-Dependent Asparagine Biosynthesis
journal, October 2007

Identification and characterisation of the selenocysteine-specific translation factor SelB from the archaeon Methanococcus jannaschii 1 1Edited by J. Karn
journal, June 2000

Structural compensation in an archaeal selenocysteine transfer RNA
journal, July 1999

Purification and properties of suppressor seryl-tRNA:ATP phosphotransferase from bovine liver
journal, April 1984

The Transamidosome: A Dynamic Ribonucleoprotein Particle Dedicated to Prokaryotic tRNA-Dependent Asparagine Biosynthesis
journal, October 2007

The 9/4 secondary structure of eukaryotic selenocysteine tRNA: More pieces of evidence
journal, September 1998

Structural and Functional Investigation of a Putative Archaeal Selenocysteine Synthase ^† ^, ^‡
journal, October 2005

Transfer Ribonucleic Acids
journal, June 1964

Crystal structure of yeast tRNAAsp
journal, December 1980

Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine
journal, February 1988

Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein
journal, November 1989

Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition
journal, May 2006

On the road to selenocysteine
journal, September 2004

RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea
journal, December 2006

A Specific Hepatic Transfer RNA for Phosphoserine
journal, October 1970

Structure of yeast phenylalanine transfer RNA at 2.5 A resolution.
journal, November 1975

Opal suppressor serine tRNAs from bovine liver form phosphoseryl-tRNA.
journal, October 1982

Differential Modes of Transfer RNASer Recognition in Methanosarcina barkeri
journal, November 2004

Characterization of mSelB, a novel mammalian elongation factor for selenoprotein translation
journal, September 2000

Compilation of tRNA sequences and sequences of tRNA genes
journal, April 1991

Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles
journal, January 1975

Unique secondary and tertiary structural features of the eucaryotic selenocysteine tRNA ^Sec
journal, January 1993

Universal rules and idiosyncratic features in tRNA identity
journal, November 1998

The characterization of phosphoseryl tRNA from lactating bovine mammary gland
journal, January 1977

ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences
journal, January 2004

The UCSC Archaeal Genome Browser
journal, January 2006

A single tRNA base pair mediates bacterial tRNA-dependent biosynthesis of asparagine
journal, October 2006

Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation
journal, January 2008

Biosynthesis of selenocysteine on its tRNA in eukaryotes
journal, April 2007

Selenocysteyl-tRNA occurs in the diatom Thalassiosira and in the ciliate Tetrahymena
journal, May 1991

Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code
journal, June 2006

Genetic analysis of selenocysteine biosynthesis in the archaeon Methanococcus maripaludis
journal, May 2011

Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator
journal, March 2019

Designing seryl‐ tRNA synthetase for improved serylation of selenocysteine tRNA s
journal, October 2018

tRNA acceptor-stem and anticodon bases embed separate features of amino acid chemistry
journal, November 2015

Non-canonical roles of tRNAs and tRNA mimics in bacterial cell biology
journal, June 2016

tRNAscan-SE 2.0: improved detection and functional classification of transfer RNA genes
journal, August 2021

A tRNA-dependent cysteine biosynthesis enzyme recognizes the selenocysteine-specific tRNA in Escherichia coli
journal, May 2010

A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro
journal, July 2015

From one amino acid to another: tRNA-dependent amino acid biosynthesis
journal, February 2008

Crystal structure of human selenocysteine tRNA
journal, August 2009

C-terminal domain of archaeal O -phosphoseryl-tRNA kinase displays large-scale motion to bind the 7-bp D-stem of archaeal tRNA Sec
journal, September 2010

Tertiary structure of bacterial selenocysteine tRNA
journal, May 2013

Kti12, a PSTK-like tRNA dependent ATPase essential for tRNA modification by Elongator
journal, March 2019

Computational identification of the selenocysteine tRNA (tRNA^Sec) in genomes
journal, February 2017