Brachypodium distachyon grain: identification and subcellular localization of storage proteins

Larré, C.; Penninck, S.; Bouchet, B.; Lollier, V.; Tranquet, O.; Denery-Papini, S.; Guillon, F.; Rogniaux, H.

doi:10.1093/jxb/erq050

Title: Brachypodium distachyon grain: identification and subcellular localization of storage proteins

Journal Article · 31 March 2010 · Journal of Experimental Botany

DOI: https://doi.org/10.1093/jxb/erq050 · OSTI ID:1625362

Larré, C. ^[1]; Penninck, S. ^[2]; Bouchet, B. ^[2]; Lollier, V. ^[2]; Tranquet, O. ^[2]; Denery-Papini, S. ^[2]; Guillon, F. ^[2]; Rogniaux, H. ^[2]

UR1268 Biopolymeres Interactions Assemblages, INRA, Nantes (France); DOE/OSTI
UR1268 Biopolymeres Interactions Assemblages, INRA, Nantes (France)

Seed storage proteins are of great importance in nutrition and in industrial transformation because of their functional properties. Brachypodium distachyon has been proposed as a new model plant to study temperate cereals. The protein composition of Brachypodium grain was investigated by separating the proteins on the basis of their solubility combined with a proteomic approach. Salt-soluble proteins as well as salt-insoluble proteins separated by two-dimensional gel electrophoresis revealed 284 and 120 spots, respectively. Proteins from the major spots were sequenced by mass spectrometry and identified by searching against a Brachypodium putative protein database. Our analysis detected globulins and prolamins but no albumins. Globulins were represented mainly by the 11S type and their solubility properties corresponded to the glutelin found in rice. An in silico search for storage proteins returned more translated genes than expressed products identified by mass spectrometry, particularly in the case of prolamin type proteins, reflecting a strong expression of globulins at the expense of prolamins. Microscopic examination of endosperm cells revealed scarce small-size starch granules surrounded by protein bodies containing 11S globulins. The presence of protein bodies containing glutelins makes B. distachyon closer to rice or oat than to wheat endosperm.

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Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth and Environmental Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625362

Journal Information:: Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 6 Vol. 61; ISSN 0022-0957

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Aleurone Cell Walls of Wheat Grain: High Spatial Resolution Investigation Using Synchrotron Infrared Microspectroscopy Jamme, F.; Robert, P.; Bouchet, B. Applied Spectroscopy, Vol. 62, Issue 8 https://doi.org/10.1366/000370208785284448	journal	August 2008
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Proteomic Analysis of Arabidopsis Seed Germination and Priming Gallardo, Karine; Job, Claudette; Groot, Steven P. C. Plant Physiology, Vol. 126, Issue 2 https://doi.org/10.1104/pp.126.2.835	journal	June 2001
Synthesis of Globulins in Maize Embryos Kriz, Alan L.; Schwartz, Drew Plant Physiology, Vol. 82, Issue 4 https://doi.org/10.1104/pp.82.4.1069	journal	December 1986
Molecular Characterization of Oat Seed Globulins Shotwell, Mark A.; Afonso, Claudio; Davies, Eric Plant Physiology, Vol. 87, Issue 3 https://doi.org/10.1104/pp.87.3.698	journal	July 1988
Nucleotide Sequence of a cDNA Clone Corresponding to the Maize Globulin-2 Gene Wallace, Nancy H.; Kriz, Alan L. Plant Physiology, Vol. 95, Issue 3 https://doi.org/10.1104/pp.95.3.973	journal	March 1991
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Seed storage proteins: structures and biosynthesis. Shewry, P. R.; Napier, J. A.; Tatham, A. S. The Plant Cell, Vol. 7, Issue 7 https://doi.org/10.1105/tpc.7.7.945	journal	July 1995
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Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis Vain, Philippe; Worland, Barbara; Thole, Vera Plant Biotechnology Journal, Vol. 6, Issue 3 https://doi.org/10.1111/j.1467-7652.2007.00308.x	journal	April 2008
Towards molecular breeding of reproductive traits in cereal crops Dwivedi, Sangam; Perotti, Enrico; Ortiz, Rodomiro Plant Biotechnology Journal, Vol. 6, Issue 6 https://doi.org/10.1111/j.1467-7652.2008.00343.x	journal	August 2008
Identification of three wheat globulin genes by screening a Triticum aestivum BAC genomic library with cDNA from a diabetes-associated globulin Loit, Evelin; Melnyk, Charles W.; MacFarlane, Amanda J. BMC Plant Biology, Vol. 9, Issue 1 https://doi.org/10.1186/1471-2229-9-93	journal	January 2009
Aleurone Cell Walls of Wheat Grain: High Spatial Resolution Investigation Using Synchrotron Infrared Microspectroscopy Jamme, F.; Robert, P.; Bouchet, B. Applied Spectroscopy, Vol. 62, Issue 8 https://doi.org/10.1366/000370208785284448	journal	August 2008

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Related Subjects

2D electrophoresis
54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
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glutelin
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prolamin
protein bodies
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Title: Brachypodium distachyon grain: identification and subcellular localization of storage proteins

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