Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia
Abstract
Cell polarity is essential for generating cell diversity and for the proper function of most differentiated cell types. In many organisms, cell polarity is regulated by the atypical protein kinase C (aPKC), Bazooka (Baz/Par3), and Par6 proteins. Here, we show that Drosophila aPKC zygotic null mutants survive to mid-larval stages, where they exhibit defects in neuroblast and epithelial cell polarity. Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large and normal spindle orientation. Mutant imaginal disc epithelia have defects in apical/basal cell polarity and tissue morphology. In addition, we show that aPKC mutants demonstrate reduced cell proliferation in both neuroblasts and epithelia, the opposite of the lethal giant larvae (lgl) tumor suppressor phenotype, and that reduced aPKC levels strongly suppress most lgl cell polarity and overproliferation phenotypes.
- Authors:
-
- Univ. of Oregon, Eugene, OR (United States). Howard Hughes Medical Institute, Institutes of Neuroscience and Molecular Biology
- Publication Date:
- Research Org.:
- Univ. of Oregon, Eugene, OR (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH); Howard Hughes Medical Institute
- OSTI Identifier:
- 1625129
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Cell Biology
- Additional Journal Information:
- Journal Volume: 163; Journal Issue: 5; Journal ID: ISSN 0021-9525
- Publisher:
- Rockefeller University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; cell biology; Lgl; asymmetric cell division; Miranda; apical/basal polarity; Par complex
Citation Formats
Rolls, Melissa M., Albertson, Roger, Shih, Hsin-Pei, Lee, Cheng-Yu, and Doe, Chris Q. Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia. United States: N. p., 2003.
Web. doi:10.1083/jcb.200306079.
Rolls, Melissa M., Albertson, Roger, Shih, Hsin-Pei, Lee, Cheng-Yu, & Doe, Chris Q. Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia. United States. https://doi.org/10.1083/jcb.200306079
Rolls, Melissa M., Albertson, Roger, Shih, Hsin-Pei, Lee, Cheng-Yu, and Doe, Chris Q. Mon .
"Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia". United States. https://doi.org/10.1083/jcb.200306079. https://www.osti.gov/servlets/purl/1625129.
@article{osti_1625129,
title = {Drosophila aPKC regulates cell polarity and cell proliferation in neuroblasts and epithelia},
author = {Rolls, Melissa M. and Albertson, Roger and Shih, Hsin-Pei and Lee, Cheng-Yu and Doe, Chris Q.},
abstractNote = {Cell polarity is essential for generating cell diversity and for the proper function of most differentiated cell types. In many organisms, cell polarity is regulated by the atypical protein kinase C (aPKC), Bazooka (Baz/Par3), and Par6 proteins. Here, we show that Drosophila aPKC zygotic null mutants survive to mid-larval stages, where they exhibit defects in neuroblast and epithelial cell polarity. Mutant neuroblasts lack apical localization of Par6 and Lgl, and fail to exclude Miranda from the apical cortex; yet, they show normal apical crescents of Baz/Par3, Pins, Inscuteable, and Discs large and normal spindle orientation. Mutant imaginal disc epithelia have defects in apical/basal cell polarity and tissue morphology. In addition, we show that aPKC mutants demonstrate reduced cell proliferation in both neuroblasts and epithelia, the opposite of the lethal giant larvae (lgl) tumor suppressor phenotype, and that reduced aPKC levels strongly suppress most lgl cell polarity and overproliferation phenotypes.},
doi = {10.1083/jcb.200306079},
journal = {Journal of Cell Biology},
number = 5,
volume = 163,
place = {United States},
year = {Mon Dec 01 00:00:00 EST 2003},
month = {Mon Dec 01 00:00:00 EST 2003}
}
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