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Title: Chromosomes are predominantly located randomly with respect to each other in interphase human cells

Journal Article · · Journal of Cell Biology
 [1];  [2];  [3];  [4];  [5];  [5];  [6];  [6];  [7];  [7];  [8]
  1. Univ. of Texas Medical Branch, Galveston, TX (United States). Dept. of Radiation Oncology; DOE/OSTI
  2. German Cancer Research Center, Heidelberg (Germany). Dept. of Skin Carcinogenesis; Technical Univ. of Munich (Germany). Dept. of Radiation Oncology
  3. Department of Radiation Oncology, University of Texas Medical Branch, Galveston, TX 77555
  4. Univ. of California, Berkeley, CA (United States). Dept. of Mathematics; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
  5. Univ. of California, Berkeley, CA (United States). Dept. of Mathematics
  6. Technical Univ. of Munich (Germany). Dept. of Radiation Oncology
  7. Harvard Medical School, Boston, MA (United States). Dana-Farber Cancer Inst.
  8. Columbia Univ., New York, NY (United States). Center for Radiological Research
+ Show Author Affiliations

To test quantitatively whether there are systematic chromosome–chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome–chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random.

Research Organization:
Univ. of Texas Medical Branch, Galveston, TX (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
FG03-98ER62684
OSTI ID:
1625127
Journal Information:
Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 2 Vol. 159; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Interchange and intra-nuclear architecture journal January 1993
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Repositioning of Human Interphase Chromosomes by Nucleolar Dynamics in the Reverse Transformation of HT1080 Fibrosarcoma Cells journal May 1998
Size-Dependent Positioning of Human Chromosomes in Interphase Nuclei journal July 2000
Rapid detection of radiation-induced chromosomal aberrations in lymphocytes and hematopoietic progenitor cells by mFISH journal July 2000
Chromatin structure and chromosome aberrations: modeling of damage induced by isotropic and localized irradiation journal August 1998
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Chromatin Motion Is Constrained by Association with Nuclear Compartments in Human Cells journal March 2002
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Review: Proximity effects in the production of chromosome aberrations by ionizing radiation journal January 1997
Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations, analysed by painting journal January 1998
Differences in the Localization and Morphology of Chromosomes in the Human Nucleus journal June 1999
The concept of self-organization in cellular architecture journal October 2001
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Spatial associations of centromeres in the nuclei of hematopoietic cells: evidence for cell-type-specific organizational patterns journal March 2000
Chromosomes exhibit preferential positioning in nuclei of quiescent human cells journal February 1999
Analyzing Radiation-Induced Complex Chromosome Rearrangements by Combinatorial Painting 1 journal May 2001
Complex Chromosome Exchanges Induced by Gamma Rays in Human Lymphocytes: An mFISH Study journal May 2001
Chromosomes Participating in Translocations Typical of Malignant Hemoblastoses Are Also Involved in Exchange Aberrations Induced by Fast Neutrons journal April 1999

Cited By (17)

Parental genomes mix in mule and human cell nuclei journal February 2009
Beyond the Sequence: Cellular Organization of Genome Function journal February 2007
Chromosome Position: Now, Where Was I? journal April 2003
The Rabl configuration limits topological entanglement of chromosomes in budding yeast journal May 2019
Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages journal February 2003
Three-Dimensional Maps of All Chromosomes in Human Male Fibroblast Nuclei and Prometaphase Rosettes journal April 2005
Modeling and Experimental Methods to Probe the Link between Global Transcription and Spatial Organization of Chromosomes journal October 2012
Clinical, Molecular- and Cytogenetic Analysis of a Case of Severe Radio- Sensitivity journal August 2012
Three-Color Chromosome Painting as Seen through the Eyes of mFISH: Another Look at Radiation-Induced Exchanges and Their Conversion to Whole-Genome Equivalency journal March 2016
Uncovering Proximity of Chromosome Territories using Classical Algebraic Statistics preprint January 2014
Prediction of radiosensitivity in human bladder cell lines using nuclear chromatin phenotype journal January 2006
Chromosome territory arrangement and homologous pairing in nuclei of Arabidopsis thaliana are predominantly random except for NOR-bearing chromosomes journal October 2004
Chromosome territory positioning of conserved homologous chromosomes in different primate species journal April 2006
Chromatin domains and the interchromatin compartment form structurally defined and functionally interacting nuclear networks journal November 2006
Live cell microscopy analysis of radiation-induced DNA double-strand break motion journal February 2009
Chromosome order in HeLa cells changes during mitosis and early G1, but is stably maintained during subsequent interphase stages journal February 2003
Three-Dimensional Maps of All Chromosomes in Human Male Fibroblast Nuclei and Prometaphase Rosettes journal April 2005

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59 BASIC BIOLOGICAL SCIENCES
Cell Biology
chromosome aberrations
human chromosome clustering
multiplex FISH
nuclear organization
proximity