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Title: Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Abstract

Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

Authors:
 [1];  [1];  [1];  [2];  [3];  [1];  [1]; ORCiD logo [4];  [5];  [5]; ORCiD logo [5];  [5];  [6]; ORCiD logo [6];  [1]
  1. Department of Biochemistry and Microbiology, University of Victoria, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada
  2. School of Biological Sciences, Victoria University, PO Box 600, Wellington, 6012, New Zealand; Department of Biochemistry and Microbiology, University of Victoria, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada
  3. Lunenfeld-Tanenbaum Research Institute, Sinai Health System, and Department of Molecular Genetics, University of Toronto, 600 University Ave, Rm 992, Toronto, ON, M5G1X5, Canada; Department of Biochemistry and Microbiology, University of Victoria, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada
  4. Marum and Max Planck Institute for Marine Microbiology, Celsiusstraße 1, 28359, Bremen, Germany; Department of Biochemistry and Microbiology, University of Victoria, PO Box 1700 STN CSC, Victoria, British Columbia, V8W 2Y2, Canada
  5. Aquatic and Crop Resource Development Research Centre, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, B3H 3Z1, Canada
  6. Department of Biological Sciences, Thompson Rivers University, 805 TRU Way, Kamloops, British Columbia, V2C 0C8, Canada
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); Natural Sciences and Engineering Research Council of Canada (NSERC); National Research Council Canada, the Canadian Institutes of Health Research; Province of Saskatchewan; Western Economic Diversification Canada; University of Saskatchewan; National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS)
OSTI Identifier:
1624536
Grant/Contract Number:  
AC02-76SF00515; FRN 04355; P41GM103393
Resource Type:
Accepted Manuscript
Journal Name:
Communications Biology
Additional Journal Information:
Journal Volume: 2; Journal Issue: 1; Journal ID: ISSN 2399-3642
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
Life Sciences & Biomedicine - Other Topics; Science & Technology - Other Topics

Citation Formats

Hettle, Andrew G., Hobbs, Joanne K., Pluvinage, Benjamin, Vickers, Chelsea, Abe, Kento T., Salama-Alber, Orly, McGuire, Bailey E., Hehemann, Jan-Hendrik, Hui, Joseph P. M., Berrue, Fabrice, Banskota, Arjun, Zhang, Junzeng, Bottos, Eric M., Van Hamme, Jonathan, and Boraston, Alisdair B.. Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species. United States: N. p., 2019. Web. https://doi.org/10.1038/s42003-019-0721-y.
Hettle, Andrew G., Hobbs, Joanne K., Pluvinage, Benjamin, Vickers, Chelsea, Abe, Kento T., Salama-Alber, Orly, McGuire, Bailey E., Hehemann, Jan-Hendrik, Hui, Joseph P. M., Berrue, Fabrice, Banskota, Arjun, Zhang, Junzeng, Bottos, Eric M., Van Hamme, Jonathan, & Boraston, Alisdair B.. Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species. United States. https://doi.org/10.1038/s42003-019-0721-y
Hettle, Andrew G., Hobbs, Joanne K., Pluvinage, Benjamin, Vickers, Chelsea, Abe, Kento T., Salama-Alber, Orly, McGuire, Bailey E., Hehemann, Jan-Hendrik, Hui, Joseph P. M., Berrue, Fabrice, Banskota, Arjun, Zhang, Junzeng, Bottos, Eric M., Van Hamme, Jonathan, and Boraston, Alisdair B.. Sun . "Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species". United States. https://doi.org/10.1038/s42003-019-0721-y. https://www.osti.gov/servlets/purl/1624536.
@article{osti_1624536,
title = {Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species},
author = {Hettle, Andrew G. and Hobbs, Joanne K. and Pluvinage, Benjamin and Vickers, Chelsea and Abe, Kento T. and Salama-Alber, Orly and McGuire, Bailey E. and Hehemann, Jan-Hendrik and Hui, Joseph P. M. and Berrue, Fabrice and Banskota, Arjun and Zhang, Junzeng and Bottos, Eric M. and Van Hamme, Jonathan and Boraston, Alisdair B.},
abstractNote = {Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-carrageenan specific polysaccharide utilization locus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.},
doi = {10.1038/s42003-019-0721-y},
journal = {Communications Biology},
number = 1,
volume = 2,
place = {United States},
year = {2019},
month = {12}
}

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