Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Hettle, Andrew G.; Hobbs, Joanne K.; Pluvinage, Benjamin; Vickers, Chelsea; Abe, Kento T.; Salama-Alber, Orly; McGuire, Bailey E.; Hehemann, Jan-Hendrik; Hui, Joseph P.  M.; Berrue, Fabrice; Banskota, Arjun; Zhang, Junzeng; Bottos, Eric M.; Van Hamme, Jonathan; Boraston, Alisdair B.

doi:10.1038/s42003-019-0721-y

Title: Insights into the κ/ι-carrageenan metabolism pathway of some marine Pseudoalteromonas species

Journal Article · 19 December 2019 · Communications Biology

DOI: https://doi.org/10.1038/s42003-019-0721-y · OSTI ID:1624536

Hettle, Andrew G. ^[1]; Hobbs, Joanne K. ^[2]; Pluvinage, Benjamin ^[2]; Vickers, Chelsea ^[2]; Abe, Kento T. ^[2]; Salama-Alber, Orly ^[2]; McGuire, Bailey E. ^[2];

^[2]; Hui, Joseph P. M. ^[3]; Berrue, Fabrice ^[3];

^[3]; Zhang, Junzeng ^[3]; Bottos, Eric M. ^[4];

^[4]; Boraston, Alisdair B. ^[2]

Univ. of Victoria, BC (Canada); DOE/OSTI
Univ. of Victoria, BC (Canada)
National Research Council of Canada, Halifax, NS (Canada)
Thompson Rivers University, Kamloops, BC (Canada)

Pseudoalteromonas is a globally distributed marine-associated genus that can be found in a broad range of aquatic environments, including in association with macroalgal surfaces where they may take advantage of these rich sources of polysaccharides. The metabolic systems that confer the ability to metabolize this abundant form of photosynthetically fixed carbon, however, are not yet fully understood. Through genomics, transcriptomics, microbiology, and specific structure-function studies of pathway components we address the capacity of newly isolated marine pseudoalteromonads to metabolize the red algal galactan carrageenan. The results reveal that the κ/ι-$$\underline{car}$$rageenan specific $$\underline{p}$$olysaccharide utilization $$\underline{l}$$ocus (CarPUL) enables isolates possessing this locus the ability to grow on this substrate. Biochemical and structural analysis of the enzymatic components of the CarPUL promoted the development of a detailed model of the κ/ι-carrageenan metabolic pathway deployed by pseudoalteromonads, thus furthering our understanding of how these microbes have adapted to a unique environmental niche.

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Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL)

Sponsoring Organization:: Canadian Institutes of Health Research (CIHR); National Institute of General Medical Sciences (NIGMS); National Institutes of Health (NIH); National Research Council Canada (NRC); Natural Sciences and Engineering Research Council of Canada (NSERC); Province of Saskatchewan; USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); University of Saskatchewan; Western Economic Diversification Canada

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1624536

Journal Information:: Communications Biology, Journal Name: Communications Biology Journal Issue: 1 Vol. 2; ISSN 2399-3642

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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