The 27 kDa Trypanosoma brucei Pentatricopeptide Repeat Protein is a G-tract Specific RNA Binding Protein

Kamba, Pakoyo F.; Dickson, David A.; White, Neil A.; Ekstrom, Jennifer L.; Koslowsky, Donna J.; Hoogstraten, Charles G.

doi:10.1038/s41598-018-34377-9

Title: The 27 kDa Trypanosoma brucei Pentatricopeptide Repeat Protein is a G-tract Specific RNA Binding Protein

Journal Article · 18 November 2018 · Scientific Reports

DOI: https://doi.org/10.1038/s41598-018-34377-9 · OSTI ID:1624435

Kamba, Pakoyo F. ^[1]; Dickson, David A. ^[2]; White, Neil A. ^[2]; Ekstrom, Jennifer L. ^[2]; Koslowsky, Donna J. ^[3];

^[2]

Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology; Michigan State Univ., East Lansing, MI (United States). Graduate Program in Cell and Molecular Biology; Makerere Univ., IKampala (Uganda); DOE/OSTI
Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology
Michigan State Univ., East Lansing, MI (United States). Graduate Program in Cell and Molecular Biology; Michigan State Univ., East Lansing, MI (United States). Dept. of Microbiology and Molecular Genetics

Pentatricopeptide repeat (PPR) proteins, a helical repeat family of organellar RNA binding proteins, play essential roles in post-transcriptional RNA processing. In Trypanosoma brucei, an expanded family of PPR proteins localize to the parasite’s single mitochondrion, where they are believed to perform important roles in both RNA processing and translation. We studied the RNA binding specificity of the simplest T. brucei PPR protein (KRIPP11) using electrophoretic mobility shift assays, fluorescence anisotropy, circular dichroism spectroscopy, and in vitro selection. We found KRIPP11 to be an RNA binding protein with specificity for sequences of four or more consecutive guanosine residues (G-tracts). Such G-tracts are dramatically enriched in T. brucei mitochondrial transcripts that are destined for extensive uridine insertion/deletion editing but are not present in mRNAs following editing. We further found that the quadruplex oligoguanosine RNA conformation is preferentially recognized by KRIPP11 over other conformational forms, and is bound without disruption of the quadruplex structure. In combination with prior data demonstrating association of KRIPP11 with the small ribosomal subunit, these results suggest possible roles for KRIPP11 in bridging mRNA maturation and translation or in facilitating translation of unusual dual-coded open reading frames.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-04ER63917

OSTI ID:: 1624435

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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