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Title: Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow’s milk allergy

Abstract

Cow’s milk allergy (CMA) is one of the earliest and most common food allergy and can be elicited by both IgE- or non-IgE-mediated mechanism. We previously described dysbiosis in children with IgEmediated CMA and the effect of dietary treatment with extensively hydrolyzed casein formula (EHCF) alone or in combination with the probiotic Lactobacillus rhamnosus GG (LGG). On the contrary, the gut microbiota in non-IgE-mediated CMA remains uncharacterized. In this study we evaluated gut microbiota composition and fecal butyrate levels in children affected by non-IgE-mediated CMA. We found a gut microbiota dysbiosis in non-IgE-mediated CMA, driven by an enrichment of Bacteroides and Alistipes. Comparing these results with those previously obtained in children with IgE-mediated CMA, we demonstrated overlapping signatures in the gut microbiota dysbiosis of non-IgE-mediated and IgE-mediated CMA children, characterized by a progressive increase in Bacteroides from healthy to IgEmediated CMA patients. EHCF containing LGG was more strongly associated with an effect on dysbiosis and on butyrate production if compared to what observed in children treated with EHCF alone. If longitudinal cohort studies in children with CMA will confirm these results, gut microbiota dysbiosis could be a relevant target for innovative therapeutic strategies in children with non-IgE-mediated CMA.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3];  [3];  [3];  [3];  [4];  [5];  [5];  [6];  [7];  [2]
+ Show Author Affiliations
  1. Univ. of Naples Federico II (Italy). Dept. of Translational Medical Science; Univ. of Naples Federico II (Italy). European Lab. for the Investigation of Food-Induced Diseases; Univ. of Naples Federico II (Italy). CEINGE Advanced Biotechnologies; Univ. of Naples Federico II (Italy). Task Force on Microbiome Studies
  2. Univ. of Naples Federico II (Italy). Task Force on Microbiome Studies; Univ. of Naples Federico II (Italy). Dept. of Agricultural Sciences
  3. Univ. of Naples Federico II (Italy). Dept. of Translational Medical Science
  4. Univ. of Naples Federico II (Italy). Dept. of Translational Medical Science; Univ. of Naples Federico II (Italy). Dept. of Agricultural Sciences
  5. Univ. of Naples Federico II (Italy). Dept. of Pharmacy
  6. Univ. of Naples Federico II (Italy). Dept. of Agricultural Sciences
  7. Argonne National Lab. (ANL), Argonne, IL (United States). Division of Bioscience
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1624415
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Science & Technology - Other Topics

Citation Formats

Berni Canani, Roberto, De Filippis, Francesca, Nocerino, Rita, Paparo, Lorella, Di Scala, Carmen, Cosenza, Linda, Della Gatta, Giusy, Calignano, Antonio, De Caro, Carmen, Laiola, Manolo, Gilbert, Jack A., and Ercolini, Danilo. Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow’s milk allergy. United States: N. p., 2018. Web. doi:10.1038/s41598-018-30428-3.
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Berni Canani, Roberto, De Filippis, Francesca, Nocerino, Rita, Paparo, Lorella, Di Scala, Carmen, Cosenza, Linda, Della Gatta, Giusy, Calignano, Antonio, De Caro, Carmen, Laiola, Manolo, Gilbert, Jack A., & Ercolini, Danilo. Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow’s milk allergy. United States. https://doi.org/10.1038/s41598-018-30428-3
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Berni Canani, Roberto, De Filippis, Francesca, Nocerino, Rita, Paparo, Lorella, Di Scala, Carmen, Cosenza, Linda, Della Gatta, Giusy, Calignano, Antonio, De Caro, Carmen, Laiola, Manolo, Gilbert, Jack A., and Ercolini, Danilo. Tue . "Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow’s milk allergy". United States. https://doi.org/10.1038/s41598-018-30428-3. https://www.osti.gov/servlets/purl/1624415.
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@article{osti_1624415,
title = {Gut microbiota composition and butyrate production in children affected by non-IgE-mediated cow’s milk allergy},
author = {Berni Canani, Roberto and De Filippis, Francesca and Nocerino, Rita and Paparo, Lorella and Di Scala, Carmen and Cosenza, Linda and Della Gatta, Giusy and Calignano, Antonio and De Caro, Carmen and Laiola, Manolo and Gilbert, Jack A. and Ercolini, Danilo},
abstractNote = {Cow’s milk allergy (CMA) is one of the earliest and most common food allergy and can be elicited by both IgE- or non-IgE-mediated mechanism. We previously described dysbiosis in children with IgEmediated CMA and the effect of dietary treatment with extensively hydrolyzed casein formula (EHCF) alone or in combination with the probiotic Lactobacillus rhamnosus GG (LGG). On the contrary, the gut microbiota in non-IgE-mediated CMA remains uncharacterized. In this study we evaluated gut microbiota composition and fecal butyrate levels in children affected by non-IgE-mediated CMA. We found a gut microbiota dysbiosis in non-IgE-mediated CMA, driven by an enrichment of Bacteroides and Alistipes. Comparing these results with those previously obtained in children with IgE-mediated CMA, we demonstrated overlapping signatures in the gut microbiota dysbiosis of non-IgE-mediated and IgE-mediated CMA children, characterized by a progressive increase in Bacteroides from healthy to IgEmediated CMA patients. EHCF containing LGG was more strongly associated with an effect on dysbiosis and on butyrate production if compared to what observed in children treated with EHCF alone. If longitudinal cohort studies in children with CMA will confirm these results, gut microbiota dysbiosis could be a relevant target for innovative therapeutic strategies in children with non-IgE-mediated CMA.},
doi = {10.1038/s41598-018-30428-3},
journal = {Scientific Reports},
number = 1,
volume = 8,
place = {United States},
year = {Tue Aug 21 00:00:00 EDT 2018},
month = {Tue Aug 21 00:00:00 EDT 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1038/s41598-018-30428-3
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Figures / Tables:

Table 1 Table 1: Main demographic and clinical features of the study population. p-values of paired t-test were reported for all variables.
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    Works referencing / citing this record:

    Gut Microbiome as Target for Innovative Strategies Against Food Allergy
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    The Two Faces of Cow’s Milk and Allergy: Induction of Cow’s Milk Allergy vs. Prevention of Asthma
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    • van Neerven, R. J. Joost; Savelkoul, Huub F. J.
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    • DOI: 10.3390/nu11081945

    Prophylactic effects of isomaltodextrin in a Balb/c mouse model of egg allergy
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    Low Concentration of Fecal Valeric Acid at 1 Year of Age Is Linked with Eczema and Food Allergy at 13 Years of Age: Findings from a Swedish Birth Cohort
    journal, November 2021

    • Gio-Batta, Monica; Spetz, Karin; Barman, Malin
    • International Archives of Allergy and Immunology, Vol. 183, Issue 4
    • DOI: 10.1159/000520149

    Gut Microbiome as Target for Innovative Strategies Against Food Allergy
    journal, February 2019

    The Two Faces of Cow’s Milk and Allergy: Induction of Cow’s Milk Allergy vs. Prevention of Asthma
    journal, August 2019

    • van Neerven, R. J. Joost; Savelkoul, Huub F. J.
    • Nutrients, Vol. 11, Issue 8
    • DOI: 10.3390/nu11081945
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