Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Vorselen, Daan; Wang, Yifan; de Jesus, Miguel M.; Shah, Pavak K.; Footer, Matthew J.; Huse, Morgan; Cai, Wei; Theriot, Julie A.

doi:10.1038/s41467-019-13804-z

Title: Microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell–target interactions

Journal Article · 06 January 2020 · Nature Communications

DOI: https://doi.org/10.1038/s41467-019-13804-z · OSTI ID:1624229

Vorselen, Daan ^[1]; Wang, Yifan ^[2];

^[3]; Shah, Pavak K. ^[4]; Footer, Matthew J. ^[5]; Huse, Morgan ^[3];

^[6];

^[5]

Stanford Univ., CA (United States). Dept. of Biochemistry; Univ. of Washington, Seattle, WA (United States). Dept. of Biology and Howard Hughes Medical Institute; DOE/OSTI
Stanford Univ., CA (United States). Dept. of Mechanical Engineering
Memorial Sloan Kettering Cancer Center, New York, NY (United States). Immunology Program
Sloan Kettering Institute, New York, NY (United States). Developmental Biology Program
Stanford Univ., CA (United States). Dept. of Biochemistry; Univ. of Washington, Seattle, WA (United States). Dept. of Biology and Howard Hughes Medical Institute
Stanford Univ., CA (United States). Dept. of Mechanical Engineering

Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical planar geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (<50 nm) accuracy using conventional confocal microscopy. We introduce a reference-free computational method allowing inference of traction forces with high sensitivity directly from the particle shape. We illustrate the potential of this approach by revealing subcellular force patterns throughout phagocytic engulfment and force dynamics in the cytotoxic T-cell immunological synapse. This strategy can readily be adapted for studying cellular forces in a wide range of applications.

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Research Organization:: Stanford Univ., CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0010412

OSTI ID:: 1624229

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 11; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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