Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization
Abstract
Broadly HIV-1 neutralizing VRC01 class antibodies target the CD4-binding site of Env. They are derived from VH1-2*02 antibody heavy chains paired with rare light chains expressing 5-amino acid-long CDRL3s. They have been isolated from infected subjects but have not yet been elicited by immunization. Env-derived immunogens capable of binding the germline forms of VRC01 B cell receptors on naive B cells have been designed and evaluated in knockin mice. However, the elicited antibodies cannot bypass glycans present on the conserved position N276 of Env, which restricts access to the CD4-binding site. Efforts to guide the appropriate maturation of these antibodies by sequential immunization have not yet been successful. Here, we report on a two-step immunization scheme that leads to the maturation of VRC01-like antibodies capable of accommodating the N276 glycan and displaying autologous tier 2 neutralizing activities. Our results are relevant to clinical trials aiming to elicit VRC01 antibodies.
- Authors:
-
- Vaccines and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Global Health, University of Washington, Seattle, WA, USA
- Vaccines and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA
- Department of Biochemistry, University of Washington, Seattle, WA, USA
- IAVI Neutralizing Antibody Center, Department of Immunology and Microbiology, The Scripps Research Institute, San Diego, CA, USA
- Department of Global Health, University of Washington, Seattle, WA, USA; Infectious Disease Research Institute, Seattle, WA, USA
- Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
- Laboratory for AIDS Vaccine Research and Development, Duke University, Durham, NC, USA
- Vaccines and Infectious Diseases Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Vaccine Research Center, National Institutes of Allergy and Infectious Diseases, NIH, Bethesda, MD, USA
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Institutes of Health (NIH); Pew Charitable Trust; Burroughs Wellcome Fund; Arnold and Mabel Beckman Cryo-EM Center; J.B. Pendleton Charitable Trust
- OSTI Identifier:
- 1623651
- Grant/Contract Number:
- AC02-05CH11231; R01 AI104384; P01 AI138212; U19 AI109632; HHSN272201800004C; R01GM120553
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Cell Reports
- Additional Journal Information:
- Journal Volume: 29; Journal Issue: 10; Journal ID: ISSN 2211-1247
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Cell Biology
Citation Formats
Parks, K. Rachael, MacCamy, Anna J., Trichka, Josephine, Gray, Matthew, Weidle, Connor, Borst, Andrew J., Khechaduri, Arineh, Takushi, Brittany, Agrawal, Parul, Guenaga, Javier, Wyatt, Richard T., Coler, Rhea, Seaman, Michael, LaBranche, Celia, Montefiori, David C., Veesler, David, Pancera, Marie, McGuire, Andrew, and Stamatatos, Leonidas. Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization. United States: N. p., 2019.
Web. doi:10.1016/j.celrep.2019.10.071.
Parks, K. Rachael, MacCamy, Anna J., Trichka, Josephine, Gray, Matthew, Weidle, Connor, Borst, Andrew J., Khechaduri, Arineh, Takushi, Brittany, Agrawal, Parul, Guenaga, Javier, Wyatt, Richard T., Coler, Rhea, Seaman, Michael, LaBranche, Celia, Montefiori, David C., Veesler, David, Pancera, Marie, McGuire, Andrew, & Stamatatos, Leonidas. Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization. United States. https://doi.org/10.1016/j.celrep.2019.10.071
Parks, K. Rachael, MacCamy, Anna J., Trichka, Josephine, Gray, Matthew, Weidle, Connor, Borst, Andrew J., Khechaduri, Arineh, Takushi, Brittany, Agrawal, Parul, Guenaga, Javier, Wyatt, Richard T., Coler, Rhea, Seaman, Michael, LaBranche, Celia, Montefiori, David C., Veesler, David, Pancera, Marie, McGuire, Andrew, and Stamatatos, Leonidas. Sun .
"Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization". United States. https://doi.org/10.1016/j.celrep.2019.10.071. https://www.osti.gov/servlets/purl/1623651.
@article{osti_1623651,
title = {Overcoming Steric Restrictions of VRC01 HIV-1 Neutralizing Antibodies through Immunization},
author = {Parks, K. Rachael and MacCamy, Anna J. and Trichka, Josephine and Gray, Matthew and Weidle, Connor and Borst, Andrew J. and Khechaduri, Arineh and Takushi, Brittany and Agrawal, Parul and Guenaga, Javier and Wyatt, Richard T. and Coler, Rhea and Seaman, Michael and LaBranche, Celia and Montefiori, David C. and Veesler, David and Pancera, Marie and McGuire, Andrew and Stamatatos, Leonidas},
abstractNote = {Broadly HIV-1 neutralizing VRC01 class antibodies target the CD4-binding site of Env. They are derived from VH1-2*02 antibody heavy chains paired with rare light chains expressing 5-amino acid-long CDRL3s. They have been isolated from infected subjects but have not yet been elicited by immunization. Env-derived immunogens capable of binding the germline forms of VRC01 B cell receptors on naive B cells have been designed and evaluated in knockin mice. However, the elicited antibodies cannot bypass glycans present on the conserved position N276 of Env, which restricts access to the CD4-binding site. Efforts to guide the appropriate maturation of these antibodies by sequential immunization have not yet been successful. Here, we report on a two-step immunization scheme that leads to the maturation of VRC01-like antibodies capable of accommodating the N276 glycan and displaying autologous tier 2 neutralizing activities. Our results are relevant to clinical trials aiming to elicit VRC01 antibodies.},
doi = {10.1016/j.celrep.2019.10.071},
journal = {Cell Reports},
number = 10,
volume = 29,
place = {United States},
year = {2019},
month = {12}
}
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Works referenced in this record:
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design
journal, May 2016
- Burton, Dennis R.; Hangartner, Lars
- Annual Review of Immunology, Vol. 34, Issue 1
Automated molecular microscopy: The new Leginon system
journal, July 2005
- Suloway, Christian; Pulokas, James; Fellmann, Denis
- Journal of Structural Biology, Vol. 151, Issue 1
Accurate determination of local defocus and specimen tilt in electron microscopy
journal, June 2003
- Mindell, Joseph A.; Grigorieff, Nikolaus
- Journal of Structural Biology, Vol. 142, Issue 3
Cloning and expression of murine Ig genes from single B cells
journal, October 2009
- Tiller, Thomas; Busse, Christian E.; Wardemann, Hedda
- Journal of Immunological Methods, Vol. 350, Issue 1-2
To EBV or not to EBV: Rational vaccine design for a common infection
journal, May 2018
- Henrickson, Sarah E.
- Science Immunology, Vol. 3, Issue 23
Unambiguous Phosphosite Localization using Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)
journal, February 2013
- Frese, Christian K.; Zhou, Houjiang; Taus, Thomas
- Journal of Proteome Research, Vol. 12, Issue 3
DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy
journal, May 2009
- Voss, N. R.; Yoshioka, C. K.; Radermacher, M.
- Journal of Structural Biology, Vol. 166, Issue 2
Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies
journal, May 2013
- Kanekiyo, Masaru; Wei, Chih-Jen; Yassine, Hadi M.
- Nature, Vol. 499, Issue 7456
A Bayesian view on cryo-EM structure determination
conference, May 2012
- Scheres, Sjors H. W.
- 2012 IEEE 9th International Symposium on Biomedical Imaging (ISBI 2012), 2012 9th IEEE International Symposium on Biomedical Imaging (ISBI)
Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation
journal, September 2010
- Mouquet, Hugo; Scheid, Johannes F.; Zoller, Markus J.
- Nature, Vol. 467, Issue 7315
Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice
journal, June 2015
- Dosenovic, Pia; von Boehmer, Lotta; Escolano, Amelia
- Cell, Vol. 161, Issue 7
Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody
journal, April 2016
- Bonsignori, Mattia; Zhou, Tongqing; Sheng, Zizhang
- Cell, Vol. 165, Issue 2
Structural basis for germ-line gene usage of a potent class of antibodies targeting the CD4-binding site of HIV-1 gp120
journal, June 2012
- West, Anthony P.; Diskin, Ron; Nussenzweig, Michel C.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 30
Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen
journal, June 2015
- Jardine, J. G.; Ota, T.; Sok, D.
- Science, Vol. 349, Issue 6244
Expanding the breadth of an HIV-1 vaccine
journal, December 2014
- Ofek, Gilad; Diskin, Ron
- Science, Vol. 346, Issue 6215
Appion: An integrated, database-driven pipeline to facilitate EM image processing
journal, April 2009
- Lander, Gabriel C.; Stagg, Scott M.; Voss, Neil R.
- Journal of Structural Biology, Vol. 166, Issue 1
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012
- Scheres, Sjors H. W.
- Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding
journal, July 2011
- Scheid, J. F.; Mouquet, H.; Ueberheide, B.
- Science, Vol. 333, Issue 6049
Sequential Immunization Elicits Broadly Neutralizing Anti-HIV-1 Antibodies in Ig Knockin Mice
journal, September 2016
- Escolano, Amelia; Steichen, Jon M.; Dosenovic, Pia
- Cell, Vol. 166, Issue 6
Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques
journal, August 2014
- Shingai, Masashi; Donau, Olivia K.; Plishka, Ronald J.
- The Journal of Experimental Medicine, Vol. 211, Issue 10
IMGT/V-QUEST: IMGT Standardized Analysis of the Immunoglobulin (IG) and T Cell Receptor (TR) Nucleotide Sequences
journal, June 2011
- Giudicelli, V.; Brochet, X.; Lefranc, M. -P.
- Cold Spring Harbor Protocols, Vol. 2011, Issue 6
Soluble Prefusion Closed DS-SOSIP.664-Env Trimers of Diverse HIV-1 Strains
journal, December 2017
- Joyce, M. Gordon; Georgiev, Ivelin S.; Yang, Yongping
- Cell Reports, Vol. 21, Issue 10
Single-particle EM reveals plasticity of interactions between the adenovirus penton base and integrin V 3
journal, June 2014
- Veesler, D.; Cupelli, K.; Burger, M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen
journal, March 2016
- Jardine, J. G.; Kulp, D. W.; Havenar-Daughton, C.
- Science, Vol. 351, Issue 6280
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques
journal, May 2019
- Escolano, Amelia; Gristick, Harry B.; Abernathy, Morgan E.
- Nature, Vol. 570, Issue 7762
The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen
journal, July 2018
- Havenar-Daughton, Colin; Sarkar, Anita; Kulp, Daniel W.
- Science Translational Medicine, Vol. 10, Issue 448
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies
journal, March 2013
- McGuire, Andrew T.; Hoot, Sam; Dreyer, Anita M.
- The Journal of Experimental Medicine, Vol. 210, Issue 4
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01
journal, July 2010
- Zhou, T.; Georgiev, I.; Wu, X.
- Science, Vol. 329, Issue 5993
Arranged Sevenfold: Structural Insights into the C-Terminal Oligomerization Domain of Human C4b-Binding Protein
journal, April 2013
- Hofmeyer, Thomas; Schmelz, Stefan; Degiacomi, Matteo T.
- Journal of Molecular Biology, Vol. 425, Issue 8
In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire
journal, December 2014
- DeKosky, Brandon J.; Kojima, Takaaki; Rodin, Alexa
- Nature Medicine, Vol. 21, Issue 1
A single injection of anti-HIV-1 antibodies protects against repeated SHIV challenges
journal, April 2016
- Gautam, Rajeev; Nishimura, Yoshiaki; Pegu, Amarendra
- Nature, Vol. 533, Issue 7601
Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies
journal, March 2014
- Doria-Rose, Nicole A.; Schramm, Chaim A.; Gorman, Jason
- Nature, Vol. 509, Issue 7498
The Oligomerization Domain of C4-Binding Protein (C4bp) Acts as an Adjuvant, and the Fusion Protein Comprised of the 19-Kilodalton Merozoite Surface Protein 1 Fused with the Murine C4bp Domain Protects Mice against Malaria
journal, May 2008
- Ogun, S. A.; Dumon-Seignovert, L.; Marchand, J. -B.
- Infection and Immunity, Vol. 76, Issue 8
A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo
journal, October 2015
- Freund, Natalia T.; Horwitz, Joshua A.; Nogueira, Lilian
- PLOS Pathogens, Vol. 11, Issue 10
Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens
journal, January 2018
- Abbott, Robert K.; Lee, Jeong Hyun; Menis, Sergey
- Immunity, Vol. 48, Issue 1
Teaching a Clone to Walk, One Step at a Time
journal, September 2016
- Crowe, James E.
- Cell, Vol. 166, Issue 6
IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis
journal, May 2008
- Brochet, X.; Lefranc, M. -P.; Giudicelli, V.
- Nucleic Acids Research, Vol. 36, Issue Web Server
Rapid and Focused Maturation of a VRC01-Class HIV Broadly Neutralizing Antibody Lineage Involves Both Binding and Accommodation of the N276-Glycan
journal, July 2019
- Umotoy, Jeffrey; Bagaya, Bernard S.; Joyce, Collin
- Immunity, Vol. 51, Issue 1
Specifically modified Env immunogens activate B-cell precursors of broadly neutralizing HIV-1 antibodies in transgenic mice
journal, February 2016
- McGuire, Andrew T.; Gray, Matthew D.; Dosenovic, Pia
- Nature Communications, Vol. 7, Issue 1
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors
journal, June 2015
- Zhou, Tongqing; Lynch, Rebecca M.; Chen, Lei
- Cell, Vol. 161, Issue 6
Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors
journal, March 2013
- Jardine, J.; Julien, J. -P.; Menis, S.
- Science, Vol. 340, Issue 6133
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus
journal, April 2013
- Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing
- Nature, Vol. 496, Issue 7446
HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure
journal, May 2018
- Kwong, Peter D.; Mascola, John R.
- Immunity, Vol. 48, Issue 5
Repertoire comparison of the B-cell receptor-encoding loci in humans and rhesus macaques by next-generation sequencing
journal, July 2016
- Vigdorovich, Vladimir; Oliver, Brian G.; Carbonetti, Sara
- Clinical & Translational Immunology, Vol. 5, Issue 7
Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs
journal, January 2013
- Hoot, Sam; McGuire, Andrew T.; Cohen, Kristen W.
- PLoS Pathogens, Vol. 9, Issue 1
Anti–HIV-1 B cell responses are dependent on B cell precursor frequency and antigen-binding affinity
journal, April 2018
- Dosenovic, Pia; Kara, Ervin E.; Pettersson, Anna-Klara
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 18
Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens
journal, January 2018
- Abbott, Robert K.; Lee, Jeong Hyun; Menis, Sergey
- Immunity, Vol. 48, Issue 1
Recent developments in the PHENIX software for automated crystallographic structure determination
journal, November 2003
- Adams, Paul D.; Gopal, Kreshna; Grosse-Kunstleve, Ralf W.
- Journal of Synchrotron Radiation, Vol. 11, Issue 1
High-Resolution Description of Antibody Heavy-Chain Repertoires in Humans
journal, August 2011
- Arnaout, Ramy; Lee, William; Cahill, Patrick
- PLoS ONE, Vol. 6, Issue 8
Vectored immunoprophylaxis protects humanized mice from mucosal HIV transmission
journal, February 2014
- Balazs, Alejandro B.; Ouyang, Yong; Hong, Christin M.
- Nature Medicine, Vol. 20, Issue 3
Maturation Pathway from Germline to Broad HIV-1 Neutralizer of a CD4-Mimic Antibody
journal, April 2016
- Bonsignori, Mattia; Zhou, Tongqing; Sheng, Zizhang
- Cell, Vol. 165, Issue 2
Inference of the HIV-1 VRC01 Antibody Lineage Unmutated Common Ancestor Reveals Alternative Pathways to Overcome a Key Glycan Barrier
journal, December 2018
- Bonsignori, Mattia; Scott, Eric; Wiehe, Kevin
- Immunity, Vol. 49, Issue 6
Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies
journal, September 2016
- Briney, Bryan; Sok, Devin; Jardine, Joseph G.
- Cell, Vol. 166, Issue 6
IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis
journal, May 2008
- Brochet, X.; Lefranc, M. -P.; Giudicelli, V.
- Nucleic Acids Research, Vol. 36, Issue Web Server
Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design
journal, May 2016
- Burton, Dennis R.; Hangartner, Lars
- Annual Review of Immunology, Vol. 34, Issue 1
In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire
journal, December 2014
- DeKosky, Brandon J.; Kojima, Takaaki; Rodin, Alexa
- Nature Medicine, Vol. 21, Issue 1
Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies
journal, March 2014
- Doria-Rose, Nicole A.; Schramm, Chaim A.; Gorman, Jason
- Nature, Vol. 509, Issue 7498
Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice
journal, June 2015
- Dosenovic, Pia; von Boehmer, Lotta; Escolano, Amelia
- Cell, Vol. 161, Issue 7
Anti–HIV-1 B cell responses are dependent on B cell precursor frequency and antigen-binding affinity
journal, April 2018
- Dosenovic, Pia; Kara, Ervin E.; Pettersson, Anna-Klara
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 18
Coot model-building tools for molecular graphics
journal, November 2004
- Emsley, Paul; Cowtan, Kevin
- Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132
Sequential Immunization Elicits Broadly Neutralizing Anti-HIV-1 Antibodies in Ig Knockin Mice
journal, September 2016
- Escolano, Amelia; Steichen, Jon M.; Dosenovic, Pia
- Cell, Vol. 166, Issue 6
Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques
journal, May 2019
- Escolano, Amelia; Gristick, Harry B.; Abernathy, Morgan E.
- Nature, Vol. 570, Issue 7762
Unambiguous Phosphosite Localization using Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)
journal, February 2013
- Frese, Christian K.; Zhou, Houjiang; Taus, Thomas
- Journal of Proteome Research, Vol. 12, Issue 3
A New Glycan-Dependent CD4-Binding Site Neutralizing Antibody Exerts Pressure on HIV-1 In Vivo
journal, October 2015
- Freund, Natalia T.; Horwitz, Joshua A.; Nogueira, Lilian
- PLOS Pathogens, Vol. 11, Issue 10
A single injection of anti-HIV-1 antibodies protects against repeated SHIV challenges
journal, April 2016
- Gautam, Rajeev; Nishimura, Yoshiaki; Pegu, Amarendra
- Nature, Vol. 533, Issue 7601
Structure-Guided Redesign Increases the Propensity of HIV Env To Generate Highly Stable Soluble Trimers
journal, December 2015
- Guenaga, Javier; Dubrovskaya, Viktoriya; de Val, Natalia
- Journal of Virology, Vol. 90, Issue 6
The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen
journal, July 2018
- Havenar-Daughton, Colin; Sarkar, Anita; Kulp, Daniel W.
- Science Translational Medicine, Vol. 10, Issue 448
Arranged Sevenfold: Structural Insights into the C-Terminal Oligomerization Domain of Human C4b-Binding Protein
journal, April 2013
- Hofmeyer, Thomas; Schmelz, Stefan; Degiacomi, Matteo T.
- Journal of Molecular Biology, Vol. 425, Issue 8
Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs
journal, January 2013
- Hoot, Sam; McGuire, Andrew T.; Cohen, Kristen W.
- PLoS Pathogens, Vol. 9, Issue 1
Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors
journal, March 2013
- Jardine, J.; Julien, J. -P.; Menis, S.
- Science, Vol. 340, Issue 6133
Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen
journal, June 2015
- Jardine, J. G.; Ota, T.; Sok, D.
- Science, Vol. 349, Issue 6244
HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen
journal, March 2016
- Jardine, J. G.; Kulp, D. W.; Havenar-Daughton, C.
- Science, Vol. 351, Issue 6280
Soluble Prefusion Closed DS-SOSIP.664-Env Trimers of Diverse HIV-1 Strains
journal, December 2017
- Joyce, M. Gordon; Georgiev, Ivelin S.; Yang, Yongping
- Cell Reports, Vol. 21, Issue 10
Self-assembling influenza nanoparticle vaccines elicit broadly neutralizing H1N1 antibodies
journal, May 2013
- Kanekiyo, Masaru; Wei, Chih-Jen; Yassine, Hadi M.
- Nature, Vol. 499, Issue 7456
Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2
journal, November 2016
- Kimanius, Dari; Forsberg, Björn O.; Scheres, Sjors HW
- eLife, Vol. 5
HIV-1 Vaccines Based on Antibody Identification, B Cell Ontogeny, and Epitope Structure
journal, May 2018
- Kwong, Peter D.; Mascola, John R.
- Immunity, Vol. 48, Issue 5
HIV-1 envelope glycan modifications that permit neutralization by germline-reverted VRC01-class broadly neutralizing antibodies
journal, November 2018
- LaBranche, Celia C.; McGuire, Andrew T.; Gray, Matthew D.
- PLOS Pathogens, Vol. 14, Issue 11
Appion: An integrated, database-driven pipeline to facilitate EM image processing
journal, April 2009
- Lander, Gabriel C.; Stagg, Scott M.; Voss, Neil R.
- Journal of Structural Biology, Vol. 166, Issue 1
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus
journal, April 2013
- Liao, Hua-Xin; Lynch, Rebecca; Zhou, Tongqing
- Nature, Vol. 496, Issue 7446
EMAN: Semiautomated Software for High-Resolution Single-Particle Reconstructions
journal, December 1999
- Ludtke, Steven J.; Baldwin, Philip R.; Chiu, Wah
- Journal of Structural Biology, Vol. 128, Issue 1
HIV-1 Fitness Cost Associated with Escape from the VRC01 Class of CD4 Binding Site Neutralizing Antibodies
journal, January 2015
- Lynch, Rebecca M.; Wong, Patrick; Tran, Lillian
- Journal of Virology, Vol. 89, Issue 8
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments
journal, February 2010
- MacLean, Brendan; Tomazela, Daniela M.; Shulman, Nicholas
- Bioinformatics, Vol. 26, Issue 7
Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies
journal, March 2013
- McGuire, Andrew T.; Hoot, Sam; Dreyer, Anita M.
- The Journal of Experimental Medicine, Vol. 210, Issue 4
Antigen modification regulates competition of broad and narrow neutralizing HIV antibodies
journal, December 2014
- McGuire, A. T.; Dreyer, A. M.; Carbonetti, S.
- Science, Vol. 346, Issue 6215
Specifically modified Env immunogens activate B-cell precursors of broadly neutralizing HIV-1 antibodies in transgenic mice
journal, February 2016
- McGuire, Andrew T.; Gray, Matthew D.; Dosenovic, Pia
- Nature Communications, Vol. 7, Issue 1
Design and crystal structure of a native-like HIV-1 envelope trimer that engages multiple broadly neutralizing antibody precursors in vivo
journal, August 2017
- Medina-Ramírez, Max; Garces, Fernando; Escolano, Amelia
- The Journal of Experimental Medicine, Vol. 214, Issue 9
Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation
journal, September 2010
- Mouquet, Hugo; Scheid, Johannes F.; Zoller, Markus J.
- Nature, Vol. 467, Issue 7315
[20] Processing of X-ray diffraction data collected in oscillation mode
book, January 1997
- Otwinowski, Zbyszek; Minor, Wladek
- Macromolecular Crystallography Part A, 307-326
Neutralizing antibodies to HIV-1 envelope protect more effectively in vivo than those to the CD4 receptor
journal, July 2014
- Pegu, A.; Yang, Z. -y.; Boyington, J. C.
- Science Translational Medicine, Vol. 6, Issue 243
Sequence and Structural Convergence of Broad and Potent HIV Antibodies That Mimic CD4 Binding
journal, July 2011
- Scheid, J. F.; Mouquet, H.; Ueberheide, B.
- Science, Vol. 333, Issue 6049
A Bayesian View on Cryo-EM Structure Determination
journal, January 2012
- Scheres, Sjors H. W.
- Journal of Molecular Biology, Vol. 415, Issue 2
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
journal, December 2012
- Scheres, Sjors H. W.
- Journal of Structural Biology, Vol. 180, Issue 3, p. 519-530
Passive transfer of modest titers of potent and broadly neutralizing anti-HIV monoclonal antibodies block SHIV infection in macaques
journal, August 2014
- Shingai, Masashi; Donau, Olivia K.; Plishka, Ronald J.
- The Journal of Experimental Medicine, Vol. 211, Issue 10
An Antibody Targeting the Fusion Machinery Neutralizes Dual-Tropic Infection and Defines a Site of Vulnerability on Epstein-Barr Virus
journal, April 2018
- Snijder, Joost; Ortego, Michael S.; Weidle, Connor
- Immunity, Vol. 48, Issue 4
Automated molecular microscopy: The new Leginon system
journal, July 2005
- Suloway, Christian; Pulokas, James; Fellmann, Denis
- Journal of Structural Biology, Vol. 151, Issue 1
Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires
journal, September 2016
- Tian, Ming; Cheng, Cheng; Chen, Xuejun
- Cell, Vol. 166, Issue 6
Cloning and expression of murine Ig genes from single B cells
journal, October 2009
- Tiller, Thomas; Busse, Christian E.; Wardemann, Hedda
- Journal of Immunological Methods, Vol. 350, Issue 1-2
Rapid and Focused Maturation of a VRC01-Class HIV Broadly Neutralizing Antibody Lineage Involves Both Binding and Accommodation of the N276-Glycan
journal, July 2019
- Umotoy, Jeffrey; Bagaya, Bernard S.; Joyce, Collin
- Immunity, Vol. 51, Issue 1
Single-particle EM reveals plasticity of interactions between the adenovirus penton base and integrin V 3
journal, June 2014
- Veesler, D.; Cupelli, K.; Burger, M.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 24
Repertoire comparison of the B-cell receptor-encoding loci in humans and rhesus macaques by next-generation sequencing
journal, July 2016
- Vigdorovich, Vladimir; Oliver, Brian G.; Carbonetti, Sara
- Clinical & Translational Immunology, Vol. 5, Issue 7
DoG Picker and TiltPicker: Software tools to facilitate particle selection in single particle electron microscopy
journal, May 2009
- Voss, N. R.; Yoshioka, C. K.; Radermacher, M.
- Journal of Structural Biology, Vol. 166, Issue 2
Structural basis for germ-line gene usage of a potent class of antibodies targeting the CD4-binding site of HIV-1 gp120
journal, June 2012
- West, Anthony P.; Diskin, Ron; Nussenzweig, Michel C.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 30
Rational Design of Envelope Identifies Broadly Neutralizing Human Monoclonal Antibodies to HIV-1
journal, July 2010
- Wu, X.; Yang, Z. -Y.; Li, Y.
- Science, Vol. 329, Issue 5993
Focused Evolution of HIV-1 Neutralizing Antibodies Revealed by Structures and Deep Sequencing
journal, August 2011
- Wu, X.; Zhou, T.; Zhu, J.
- Science, Vol. 333, Issue 6049
Differences in Allelic Frequency and CDRH3 Region Limit the Engagement of HIV Env Immunogens by Putative VRC01 Neutralizing Antibody Precursors
journal, November 2016
- Yacoob, Christina; Pancera, Marie; Vigdorovich, Vladimir
- Cell Reports, Vol. 17, Issue 6
Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01
journal, July 2010
- Zhou, T.; Georgiev, I.; Wu, X.
- Science, Vol. 329, Issue 5993
Multidonor Analysis Reveals Structural Elements, Genetic Determinants, and Maturation Pathway for HIV-1 Neutralization by VRC01-Class Antibodies
journal, August 2013
- Zhou, Tongqing; Zhu, Jiang; Wu, Xueling
- Immunity, Vol. 39, Issue 2
Structural Repertoire of HIV-1-Neutralizing Antibodies Targeting the CD4 Supersite in 14 Donors
journal, June 2015
- Zhou, Tongqing; Lynch, Rebecca M.; Chen, Lei
- Cell, Vol. 161, Issue 6
New tools for automated high-resolution cryo-EM structure determination in RELION-3
journal, November 2018
- Zivanov, Jasenko; Nakane, Takanori; Forsberg, Björn O.
- eLife, Vol. 7