Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120

López-Igual, Rocío; Wilson, Adjélé; Leverenz, Ryan L.; Melnicki, Matthew R.; Bourcier de Carbon, Céline; Sutter, Markus; Turmo, Aiko; Perreau, François; Kerfeld, Cheryl A.; Kirilovsky, Diana

doi:10.1104/pp.16.00502

Title: Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120

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Abstract

The photoactive Orange Carotenoid Protein (OCP) is involved in cyanobacterial photoprotection. Its N-terminal domain (NTD) is responsible for interaction with the antenna and induction of excitation energy quenching, while the C-terminal domain is the regulatory domain that senses light and induces photoactivation. In most nitrogen-fixing cyanobacterial strains, there are one to four paralogous genes coding for homologs to the NTD of the OCP. The functions of these proteins are unknown. Here, we study the expression, localization, and function of these genes in Anabaena sp. PCC 7120. We show that the four genes present in the genome are expressed in both vegetative cells and heterocysts but do not seem to have an essential role in heterocyst formation. This study establishes that all four Anabaena NTD-like proteins can bind a carotenoid and the different paralogs have distinct functions. Surprisingly, only one paralog (All4941) was able to interact with the antenna and to induce permanent thermal energy dissipation. Two of the other Anabaena paralogs (All3221 and Alr4783) were shown to be very good singlet oxygen quenchers. The fourth paralog (All1123) does not seem to be involved in photoprotection. Structural homology modeling allowed us to propose specific features responsible for the different functions ofmore »« less

Authors:

López-Igual, Rocío ^[1]; Wilson, Adjélé;

;

; Bourcier de Carbon, Céline; Sutter, Markus; Turmo, Aiko;

;

Institute for Integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique, CNRS, Université Paris-Sud, Université Paris-Saclay, 91198 Gif sur Yvette, France (R.L.-I., A.W., C.B.d.C., D.K.),

Publication Date:: Fri May 13 00:00:00 EDT 2016

Research Org.:: Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory; University of California, Berkeley, CA (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1619898

Alternate Identifier(s):: OSTI ID: 1603357

Grant/Contract Number:: FG02-91ER20021; AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Plant Physiology (Bethesda)

Additional Journal Information:: Journal Name: Plant Physiology (Bethesda) Journal Volume: 171 Journal Issue: 3; Journal ID: ISSN 0032-0889

Publisher:: Oxford University Press

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    López-Igual, Rocío, Wilson, Adjélé, Leverenz, Ryan L., Melnicki, Matthew R., Bourcier de Carbon, Céline, Sutter, Markus, Turmo, Aiko, Perreau, François, Kerfeld, Cheryl A., and Kirilovsky, Diana. Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120.  United States: N. p., 2016. 
Web.  doi:10.1104/pp.16.00502.

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                    López-Igual, Rocío, Wilson, Adjélé, Leverenz, Ryan L., Melnicki, Matthew R., Bourcier de Carbon, Céline, Sutter, Markus, Turmo, Aiko, Perreau, François, Kerfeld, Cheryl A., & Kirilovsky, Diana. Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120.  United States.  https://doi.org/10.1104/pp.16.00502

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                    López-Igual, Rocío, Wilson, Adjélé, Leverenz, Ryan L., Melnicki, Matthew R., Bourcier de Carbon, Céline, Sutter, Markus, Turmo, Aiko, Perreau, François, Kerfeld, Cheryl A., and Kirilovsky, Diana. Fri .  
"Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120".  United States.  https://doi.org/10.1104/pp.16.00502.

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@article{osti_1619898,

  title        = {Different Functions of the Paralogs to the N-Terminal Domain of the Orange Carotenoid Protein in the Cyanobacterium Anabaena sp. PCC 7120},

  author       = {López-Igual, Rocío and Wilson, Adjélé and Leverenz, Ryan L. and Melnicki, Matthew R. and Bourcier de Carbon, Céline and Sutter, Markus and Turmo, Aiko and Perreau, François and Kerfeld, Cheryl A. and Kirilovsky, Diana},

  abstractNote = {The photoactive Orange Carotenoid Protein (OCP) is involved in cyanobacterial photoprotection. Its N-terminal domain (NTD) is responsible for interaction with the antenna and induction of excitation energy quenching, while the C-terminal domain is the regulatory domain that senses light and induces photoactivation. In most nitrogen-fixing cyanobacterial strains, there are one to four paralogous genes coding for homologs to the NTD of the OCP. The functions of these proteins are unknown. Here, we study the expression, localization, and function of these genes in Anabaena sp. PCC 7120. We show that the four genes present in the genome are expressed in both vegetative cells and heterocysts but do not seem to have an essential role in heterocyst formation. This study establishes that all four Anabaena NTD-like proteins can bind a carotenoid and the different paralogs have distinct functions. Surprisingly, only one paralog (All4941) was able to interact with the antenna and to induce permanent thermal energy dissipation. Two of the other Anabaena paralogs (All3221 and Alr4783) were shown to be very good singlet oxygen quenchers. The fourth paralog (All1123) does not seem to be involved in photoprotection. Structural homology modeling allowed us to propose specific features responsible for the different functions of these soluble carotenoid-binding proteins.},

  doi          = {10.1104/pp.16.00502},

  journal      = {Plant Physiology (Bethesda)},

  number       = 3,

  volume       = 171,

  place        = {United States},

  year         = {Fri May 13 00:00:00 EDT 2016},

  month        = {Fri May 13 00:00:00 EDT 2016}

}

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